Bdellovibrio phage phi1402

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6LQE5|F6LQE5_9CAUD Uncharacterized protein OS=Bdellovibrio phage phi1402 OX=1035662 PE=4 SV=1
MM1 pKa = 7.28QFFKK5 pKa = 10.58STDD8 pKa = 3.13ADD10 pKa = 3.59EE11 pKa = 4.29QKK13 pKa = 10.49FYY15 pKa = 11.21QIAASTMDD23 pKa = 3.13ILEE26 pKa = 4.32AQEE29 pKa = 3.89NAFFAEE35 pKa = 5.26AYY37 pKa = 10.17ACFILGQVLYY47 pKa = 8.67DD48 pKa = 3.89TGASTISTAIPRR60 pKa = 11.84YY61 pKa = 8.97IFRR64 pKa = 11.84TAFFAIFDD72 pKa = 4.08SFVEE76 pKa = 4.58GGSLEE81 pKa = 4.51SYY83 pKa = 6.98MTVFRR88 pKa = 11.84AIFGDD93 pKa = 3.97SVSVEE98 pKa = 4.18FEE100 pKa = 4.18VPQPGVLYY108 pKa = 10.52INIQGLEE115 pKa = 4.36YY116 pKa = 9.24ITEE119 pKa = 5.49PITGRR124 pKa = 11.84RR125 pKa = 11.84IVDD128 pKa = 3.19NKK130 pKa = 10.66YY131 pKa = 10.84VYY133 pKa = 11.04DD134 pKa = 6.42DD135 pKa = 3.98ILDD138 pKa = 4.02SNGEE142 pKa = 4.02QILAQTTMGVKK153 pKa = 7.75TQKK156 pKa = 11.11DD157 pKa = 3.39MDD159 pKa = 5.16IIMPEE164 pKa = 4.31LTPEE168 pKa = 4.75GIFVQTTLTFSS179 pKa = 3.32

Molecular weight: 20.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6LQC3|F6LQC3_9CAUD Putative RNA-binding protein OS=Bdellovibrio phage phi1402 OX=1035662 PE=4 SV=1
MM1 pKa = 8.03KK2 pKa = 10.1IGQLAALIAKK12 pKa = 9.99KK13 pKa = 9.85EE14 pKa = 4.08GHH16 pKa = 6.34KK17 pKa = 10.47SEE19 pKa = 4.81SRR21 pKa = 11.84IGDD24 pKa = 3.15IRR26 pKa = 11.84EE27 pKa = 3.9VLAILSDD34 pKa = 3.88LSYY37 pKa = 11.37GSPEE41 pKa = 3.92PLNAIVKK48 pKa = 10.34NGIARR53 pKa = 11.84AKK55 pKa = 9.95KK56 pKa = 9.79KK57 pKa = 10.67SKK59 pKa = 10.38NGQAKK64 pKa = 8.2GQKK67 pKa = 7.37GHH69 pKa = 6.87RR70 pKa = 11.84ARR72 pKa = 11.84ARR74 pKa = 11.84AA75 pKa = 3.31

Molecular weight: 8.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

7498

56

514

178.5

19.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.216 ± 0.552

1.04 ± 0.188

5.735 ± 0.237

6.282 ± 0.349

4.615 ± 0.257

7.882 ± 0.31

1.347 ± 0.167

5.762 ± 0.326

7.442 ± 0.498

8.082 ± 0.286

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.494 ± 0.152

4.348 ± 0.365

4.575 ± 0.365

4.014 ± 0.259

3.894 ± 0.39

6.202 ± 0.312

6.242 ± 0.487

6.468 ± 0.277

1.147 ± 0.218

3.214 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski