Streptomyces phage Romero

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Beephvirinae; Immanueltrevirus; unclassified Immanueltrevirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5BMD2|A0A2H5BMD2_9CAUD Uncharacterized protein OS=Streptomyces phage Romero OX=2060088 GN=SEA_ROMERO_44 PE=4 SV=1
MM1 pKa = 7.39SEE3 pKa = 4.42AYY5 pKa = 10.12LVLNEE10 pKa = 4.18TTFVSTDD17 pKa = 3.1QVTTEE22 pKa = 4.19PSSLHH27 pKa = 5.38EE28 pKa = 4.24TLSGAVKK35 pKa = 9.93WLAEE39 pKa = 3.99LAEE42 pKa = 4.0QHH44 pKa = 6.17GVDD47 pKa = 4.14VEE49 pKa = 4.06EE50 pKa = 5.26DD51 pKa = 3.56ANSVYY56 pKa = 10.36IPAPPGSGIEE66 pKa = 3.82TDD68 pKa = 3.11EE69 pKa = 4.29YY70 pKa = 11.27YY71 pKa = 10.2IIEE74 pKa = 4.49MEE76 pKa = 4.86LKK78 pKa = 10.6DD79 pKa = 3.56

Molecular weight: 8.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5BMF3|A0A2H5BMF3_9CAUD Uncharacterized protein OS=Streptomyces phage Romero OX=2060088 GN=SEA_ROMERO_78 PE=4 SV=1
MM1 pKa = 7.25NRR3 pKa = 11.84RR4 pKa = 11.84NKK6 pKa = 9.9SSKK9 pKa = 9.81VRR11 pKa = 11.84TKK13 pKa = 10.65CSHH16 pKa = 6.59CKK18 pKa = 8.78VTMSSDD24 pKa = 3.07KK25 pKa = 10.51PHH27 pKa = 7.27RR28 pKa = 11.84PNCKK32 pKa = 9.4VKK34 pKa = 10.56NNEE37 pKa = 4.18HH38 pKa = 5.24GWPRR42 pKa = 11.84EE43 pKa = 4.04TEE45 pKa = 3.79KK46 pKa = 10.46TRR48 pKa = 11.84LYY50 pKa = 11.2GRR52 pKa = 4.35

Molecular weight: 6.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13353

40

1648

208.6

22.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.975 ± 0.414

0.756 ± 0.145

6.298 ± 0.32

5.931 ± 0.296

3.422 ± 0.174

8.979 ± 0.316

1.61 ± 0.129

4.254 ± 0.201

5.624 ± 0.41

6.89 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.838 ± 0.251

3.924 ± 0.215

4.261 ± 0.225

4.696 ± 0.261

4.943 ± 0.264

6.396 ± 0.296

6.748 ± 0.367

7.115 ± 0.359

1.722 ± 0.12

3.617 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski