Ruminiclostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) (Clostridium cellulolyticum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminiclostridium; Ruminiclostridium cellulolyticum

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3287 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8I8E5|B8I8E5_RUMCH Uncharacterized protein OS=Ruminiclostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) OX=394503 GN=Ccel_2951 PE=4 SV=1
MM1 pKa = 7.77QEE3 pKa = 3.78EE4 pKa = 4.7NLTISIWDD12 pKa = 3.63RR13 pKa = 11.84EE14 pKa = 4.33SPINGVSAEE23 pKa = 4.67EE24 pKa = 3.82ILSYY28 pKa = 11.07CDD30 pKa = 5.53DD31 pKa = 3.59IAKK34 pKa = 10.39EE35 pKa = 3.92PVVLLFSDD43 pKa = 4.41PDD45 pKa = 3.78TGHH48 pKa = 6.42VNQIQFPNTLVNILNTHH65 pKa = 7.08DD66 pKa = 3.43SFKK69 pKa = 10.67PVNEE73 pKa = 4.05TDD75 pKa = 4.38PIVIGHH81 pKa = 7.72LYY83 pKa = 10.07LQYY86 pKa = 11.08LYY88 pKa = 10.42LINHH92 pKa = 5.95TEE94 pKa = 3.98PPKK97 pKa = 10.78SEE99 pKa = 3.97LQILQEE105 pKa = 4.35TVDD108 pKa = 3.82TLVADD113 pKa = 4.12NLNLQAQIDD122 pKa = 4.02TLITSNLQGG131 pKa = 3.42

Molecular weight: 14.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8I1L0|B8I1L0_RUMCH Peptidase C26 OS=Ruminiclostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) OX=394503 GN=Ccel_3356 PE=4 SV=1
MM1 pKa = 7.56LSAIQHH7 pKa = 5.8IDD9 pKa = 2.99IFILNLIQNNLHH21 pKa = 6.2SPFMDD26 pKa = 3.14KK27 pKa = 10.91LMPAITFLGNGGAIWVIIALLLVVSKK53 pKa = 9.88KK54 pKa = 10.17HH55 pKa = 5.34RR56 pKa = 11.84VTGIMVIGALIICLIIGNFTLKK78 pKa = 10.55PIIARR83 pKa = 11.84ARR85 pKa = 11.84PCWVNTNIQLLVSSPRR101 pKa = 11.84DD102 pKa = 3.21YY103 pKa = 11.27SFPSGHH109 pKa = 5.94TMSSFAVATVLFLRR123 pKa = 11.84NRR125 pKa = 11.84RR126 pKa = 11.84YY127 pKa = 10.36GLWALLLAALISFSRR142 pKa = 11.84LYY144 pKa = 10.86LYY146 pKa = 9.91VHH148 pKa = 6.32YY149 pKa = 9.68PSDD152 pKa = 3.58VAAGLVLGVAASCLSLKK169 pKa = 10.43AIPAIINSSVKK180 pKa = 9.38IQKK183 pKa = 7.36TRR185 pKa = 11.84RR186 pKa = 3.38

Molecular weight: 20.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3287

0

3287

1145150

31

5854

348.4

39.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.403 ± 0.04

1.236 ± 0.016

5.588 ± 0.03

6.644 ± 0.046

4.234 ± 0.032

6.872 ± 0.043

1.447 ± 0.018

8.62 ± 0.046

7.829 ± 0.041

8.779 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.593 ± 0.021

5.671 ± 0.033

3.267 ± 0.022

2.97 ± 0.024

3.785 ± 0.03

6.77 ± 0.032

5.561 ± 0.053

6.76 ± 0.042

0.902 ± 0.017

4.069 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski