Alfalfa latent virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus; Pea streak virus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5H7M3|A0A0C5H7M3_9VIRU Helicase OS=Alfalfa latent virus OX=165250 PE=3 SV=1
MM1 pKa = 7.47QLFQVILSVFSCCAVLLCLYY21 pKa = 10.07TIDD24 pKa = 4.58SFLNNSVCQCTVVLTGEE41 pKa = 4.12SVKK44 pKa = 10.54IVGCEE49 pKa = 3.77FTSEE53 pKa = 4.24FIEE56 pKa = 4.26YY57 pKa = 10.66AKK59 pKa = 9.63TLKK62 pKa = 10.35VQAII66 pKa = 4.02

Molecular weight: 7.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q913Z7|Q913Z7_9VIRU Movement protein TGB2 OS=Alfalfa latent virus OX=165250 PE=3 SV=1
MM1 pKa = 7.61PLIAPPNNSNSYY13 pKa = 9.8LALAIGAGFAIIIFTLRR30 pKa = 11.84SNQLPHH36 pKa = 7.87VGDD39 pKa = 4.79NIHH42 pKa = 6.42SLPHH46 pKa = 5.56GGFYY50 pKa = 10.28RR51 pKa = 11.84DD52 pKa = 3.42GTKK55 pKa = 10.29VIQYY59 pKa = 8.2NSPVRR64 pKa = 11.84TPNNWFKK71 pKa = 11.32GPNNIQALALVLLVIGLIHH90 pKa = 6.83ASSVKK95 pKa = 9.98ISRR98 pKa = 11.84GCSCSKK104 pKa = 10.9

Molecular weight: 11.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2616

66

1908

523.2

59.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.766 ± 1.24

2.638 ± 0.389

5.505 ± 0.436

7.989 ± 0.968

5.81 ± 0.31

6.078 ± 0.266

2.332 ± 0.518

5.925 ± 0.637

6.46 ± 0.451

10.512 ± 1.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.911 ± 0.276

4.472 ± 0.503

3.517 ± 0.664

3.058 ± 0.733

5.505 ± 0.395

7.148 ± 0.489

4.205 ± 0.413

6.078 ± 0.781

0.994 ± 0.241

3.096 ± 0.647
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski