Pepper enamovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Enamovirus; unclassified Enamovirus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I7UGC7|A0A2I7UGC7_9LUTE ORF0 OS=Pepper enamovirus OX=2073163 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.26ISTAEE7 pKa = 3.74NDD9 pKa = 3.18RR10 pKa = 11.84DD11 pKa = 3.56IVAKK15 pKa = 9.93SFNYY19 pKa = 9.84ARR21 pKa = 11.84FYY23 pKa = 10.82KK24 pKa = 10.26WEE26 pKa = 4.42DD27 pKa = 3.84DD28 pKa = 3.01KK29 pKa = 11.8WEE31 pKa = 4.24TVNLSAGFASNDD43 pKa = 3.48SEE45 pKa = 4.43KK46 pKa = 10.38AQPYY50 pKa = 10.04IIVPADD56 pKa = 3.0KK57 pKa = 11.17GKK59 pKa = 9.4FQVYY63 pKa = 8.99LEE65 pKa = 4.56CNGFQAVKK73 pKa = 10.5SIGGKK78 pKa = 9.98ADD80 pKa = 3.65GCWSGLVAYY89 pKa = 7.75EE90 pKa = 3.79ATGPGWLVNQYY101 pKa = 9.76VGCTITNYY109 pKa = 9.22QSSMKK114 pKa = 9.43FVCGHH119 pKa = 7.22PDD121 pKa = 3.18VEE123 pKa = 5.14LNGCKK128 pKa = 10.17FKK130 pKa = 11.26ANRR133 pKa = 11.84GVEE136 pKa = 3.78ADD138 pKa = 5.08FYY140 pKa = 11.66ASFDD144 pKa = 4.58LEE146 pKa = 5.76ADD148 pKa = 3.94DD149 pKa = 6.67DD150 pKa = 4.59DD151 pKa = 5.48SKK153 pKa = 11.27WVLYY157 pKa = 10.86APDD160 pKa = 3.74LRR162 pKa = 11.84KK163 pKa = 10.54DD164 pKa = 2.85SDD166 pKa = 3.77YY167 pKa = 11.3NYY169 pKa = 8.38VVSYY173 pKa = 10.9GGYY176 pKa = 7.21TDD178 pKa = 5.24KK179 pKa = 10.84ICEE182 pKa = 4.16VGSISISIDD191 pKa = 3.12EE192 pKa = 4.41VNAEE196 pKa = 4.08GSGSNAHH203 pKa = 5.66SALWRR208 pKa = 11.84LSEE211 pKa = 5.13PIDD214 pKa = 3.67KK215 pKa = 10.47EE216 pKa = 4.21KK217 pKa = 11.08SLKK220 pKa = 10.74LPLKK224 pKa = 10.43RR225 pKa = 11.84RR226 pKa = 11.84SCWSSDD232 pKa = 3.31SEE234 pKa = 4.35

Molecular weight: 26.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I7UGD2|A0A2I7UGD2_9LUTE Coat protein OS=Pepper enamovirus OX=2073163 PE=4 SV=1
MM1 pKa = 7.61ANNGRR6 pKa = 11.84RR7 pKa = 11.84NRR9 pKa = 11.84PRR11 pKa = 11.84RR12 pKa = 11.84AKK14 pKa = 9.82KK15 pKa = 10.02APRR18 pKa = 11.84AAPVVVVRR26 pKa = 11.84NAPARR31 pKa = 11.84PRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SGGGRR40 pKa = 11.84RR41 pKa = 11.84SRR43 pKa = 11.84RR44 pKa = 11.84GGGAGEE50 pKa = 3.67WRR52 pKa = 11.84PYY54 pKa = 10.36HH55 pKa = 7.27LYY57 pKa = 10.59GLKK60 pKa = 10.96GNDD63 pKa = 2.92KK64 pKa = 10.99GYY66 pKa = 8.98LTFGPDD72 pKa = 3.12GQTPSLGGGTLKK84 pKa = 10.82ACAEE88 pKa = 4.21YY89 pKa = 10.45KK90 pKa = 9.27ITALKK95 pKa = 9.49VQWKK99 pKa = 8.28SQASATVNGSMAIEE113 pKa = 4.67LGLGSTITTVSSRR126 pKa = 11.84ATSFKK131 pKa = 10.83LSVSGSKK138 pKa = 10.38SFSGKK143 pKa = 9.53EE144 pKa = 3.53LGATGRR150 pKa = 11.84MLPTGDD156 pKa = 3.67SASGEE161 pKa = 3.94KK162 pKa = 10.92GEE164 pKa = 4.17NQFRR168 pKa = 11.84LAYY171 pKa = 9.9NGNGPADD178 pKa = 3.37VCGDD182 pKa = 4.06LLCFFKK188 pKa = 10.78LQCAMPKK195 pKa = 10.31

Molecular weight: 20.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1936

195

1183

484.0

53.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.471 ± 0.316

2.634 ± 0.236

4.752 ± 0.993

6.87 ± 0.836

3.977 ± 0.233

8.419 ± 1.224

1.653 ± 0.468

3.254 ± 0.405

4.855 ± 1.096

10.176 ± 1.382

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.446 ± 0.219

2.996 ± 0.691

6.147 ± 0.886

3.461 ± 0.676

6.457 ± 0.942

8.523 ± 0.53

4.7 ± 0.5

6.302 ± 0.584

2.479 ± 0.356

2.428 ± 0.992
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski