Nosema ceranae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Apansporoblastina; Nosematidae; Nosema

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3200 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F9ZD82|A0A0F9ZD82_9MICR Ankyrin repeat domain-containing protein 32 OS=Nosema ceranae OX=40302 GN=AAJ76_1800038833 PE=4 SV=1
MM1 pKa = 7.14TKK3 pKa = 10.23FYY5 pKa = 10.34PSYY8 pKa = 10.76KK9 pKa = 10.38DD10 pKa = 3.52YY11 pKa = 11.21FEE13 pKa = 4.83KK14 pKa = 11.02LQDD17 pKa = 3.48IQDD20 pKa = 3.56LDD22 pKa = 4.37FYY24 pKa = 11.65DD25 pKa = 3.98EE26 pKa = 4.33VDD28 pKa = 3.28IKK30 pKa = 11.45DD31 pKa = 3.52FTFDD35 pKa = 3.87KK36 pKa = 11.04DD37 pKa = 3.64NSRR40 pKa = 11.84LTYY43 pKa = 9.61PCPCGDD49 pKa = 3.5LFEE52 pKa = 5.61IFIEE56 pKa = 4.38DD57 pKa = 4.13LLNHH61 pKa = 6.7EE62 pKa = 4.99DD63 pKa = 3.55VARR66 pKa = 11.84CPSCSLIIKK75 pKa = 9.88IVYY78 pKa = 9.9EE79 pKa = 4.88DD80 pKa = 3.7IDD82 pKa = 5.03LEE84 pKa = 4.62DD85 pKa = 4.02FLL87 pKa = 6.81

Molecular weight: 10.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F9WT44|A0A0F9WT44_9MICR Transcription elongation factor s-ii OS=Nosema ceranae OX=40302 GN=AAJ76_800039929 PE=4 SV=1
MM1 pKa = 6.74YY2 pKa = 9.11TQGDD6 pKa = 3.92NPRR9 pKa = 11.84VDD11 pKa = 3.27EE12 pKa = 4.27SRR14 pKa = 11.84RR15 pKa = 11.84SGEE18 pKa = 3.66NSSRR22 pKa = 11.84RR23 pKa = 11.84TSRR26 pKa = 11.84AIVNKK31 pKa = 10.01LHH33 pKa = 6.24FNNMSSHH40 pKa = 5.91SKK42 pKa = 8.18SHH44 pKa = 6.1RR45 pKa = 11.84TSQTGPRR52 pKa = 11.84ILSAIEE58 pKa = 3.93IFLSTGRR65 pKa = 11.84IPNSYY70 pKa = 9.04STMNLCKK77 pKa = 10.45FCWSS81 pKa = 3.41

Molecular weight: 9.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3200

0

3200

832824

32

3393

260.3

30.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.015 ± 0.031

2.086 ± 0.022

5.699 ± 0.036

6.799 ± 0.049

6.14 ± 0.048

3.491 ± 0.043

1.668 ± 0.018

9.664 ± 0.048

10.559 ± 0.058

9.949 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.038 ± 0.017

7.967 ± 0.047

2.39 ± 0.03

2.71 ± 0.024

3.449 ± 0.032

6.999 ± 0.044

4.832 ± 0.035

5.108 ± 0.035

0.583 ± 0.011

4.853 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski