Beihai weivirus-like virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KL49|A0A1L3KL49_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 3 OX=1922751 PE=4 SV=1
MM1 pKa = 7.31LASVPSGITWAYY13 pKa = 8.99DD14 pKa = 3.37APPEE18 pKa = 3.96LLEE21 pKa = 4.24IAGNRR26 pKa = 11.84AGSWKK31 pKa = 8.02ITTWEE36 pKa = 4.23FVHH39 pKa = 7.04PDD41 pKa = 3.13KK42 pKa = 11.27KK43 pKa = 10.58KK44 pKa = 10.63LKK46 pKa = 7.95STSVILTEE54 pKa = 5.18HH55 pKa = 6.69EE56 pKa = 4.61FNSLNATARR65 pKa = 11.84QCIASASKK73 pKa = 9.95EE74 pKa = 3.87QVLRR78 pKa = 11.84GSLEE82 pKa = 3.72QTAARR87 pKa = 11.84MWKK90 pKa = 8.5TGGLEE95 pKa = 4.01DD96 pKa = 3.81VALRR100 pKa = 11.84TLGPEE105 pKa = 4.06LVLRR109 pKa = 11.84WLRR112 pKa = 11.84AGQVQTGLTFHH123 pKa = 6.79FRR125 pKa = 11.84MGVPLSILSVLGACCCGSWRR145 pKa = 11.84TIVAILAKK153 pKa = 10.08KK154 pKa = 8.55VVKK157 pKa = 10.21FRR159 pKa = 11.84SIDD162 pKa = 3.43LRR164 pKa = 11.84LGLLVAAALLWDD176 pKa = 4.04AWRR179 pKa = 11.84SAGTYY184 pKa = 8.34RR185 pKa = 11.84TILRR189 pKa = 11.84WVGPSSSKK197 pKa = 9.39TSKK200 pKa = 9.83EE201 pKa = 3.86KK202 pKa = 10.89KK203 pKa = 7.62PAKK206 pKa = 9.82AAEE209 pKa = 4.41IKK211 pKa = 10.5DD212 pKa = 4.21DD213 pKa = 4.3PPKK216 pKa = 10.94ADD218 pKa = 4.55AGPSGDD224 pKa = 3.73KK225 pKa = 10.94VADD228 pKa = 3.8PSVSEE233 pKa = 4.0EE234 pKa = 3.88AEE236 pKa = 3.94ARR238 pKa = 11.84AKK240 pKa = 9.94PQEE243 pKa = 4.16NADD246 pKa = 3.67LAVRR250 pKa = 11.84TEE252 pKa = 4.01EE253 pKa = 3.82QEE255 pKa = 4.07ARR257 pKa = 11.84VTMEE261 pKa = 3.1GHH263 pKa = 4.54GRR265 pKa = 11.84YY266 pKa = 9.11RR267 pKa = 11.84IQMIEE272 pKa = 4.04DD273 pKa = 3.36KK274 pKa = 10.46KK275 pKa = 9.52VCVVLGQDD283 pKa = 3.49YY284 pKa = 10.87DD285 pKa = 4.08KK286 pKa = 11.33SVPKK290 pKa = 10.57DD291 pKa = 3.34RR292 pKa = 11.84FPDD295 pKa = 3.26VGVIVAPCSDD305 pKa = 4.29LPNVYY310 pKa = 9.5TNSAGNVHH318 pKa = 6.55HH319 pKa = 6.67GVEE322 pKa = 3.8EE323 pKa = 3.98RR324 pKa = 11.84LIKK327 pKa = 10.23KK328 pKa = 8.97SKK330 pKa = 8.77PCTWTKK336 pKa = 11.11DD337 pKa = 3.4DD338 pKa = 4.05KK339 pKa = 11.67LKK341 pKa = 10.78VGKK344 pKa = 10.08FIYY347 pKa = 10.28AAMGPKK353 pKa = 10.35GIFSTDD359 pKa = 3.17RR360 pKa = 11.84VRR362 pKa = 11.84SWYY365 pKa = 9.32EE366 pKa = 3.32SHH368 pKa = 7.5FALEE372 pKa = 4.73DD373 pKa = 3.42MRR375 pKa = 11.84SSKK378 pKa = 9.9WSEE381 pKa = 3.68SRR383 pKa = 11.84FVSTFEE389 pKa = 4.14GLLAQVNPTFKK400 pKa = 10.78FKK402 pKa = 9.98TAVKK406 pKa = 9.72AEE408 pKa = 4.04HH409 pKa = 6.12MPEE412 pKa = 3.85GKK414 pKa = 10.28APRR417 pKa = 11.84FLIADD422 pKa = 3.64GDD424 pKa = 4.08EE425 pKa = 4.49GQVLALAAVKK435 pKa = 10.51CMEE438 pKa = 4.09EE439 pKa = 3.91VLFEE443 pKa = 4.23VMEE446 pKa = 4.26SHH448 pKa = 7.44SIKK451 pKa = 10.47HH452 pKa = 4.58VCKK455 pKa = 10.17QEE457 pKa = 3.52AMKK460 pKa = 10.65RR461 pKa = 11.84LLGHH465 pKa = 6.1MVPPNAAKK473 pKa = 10.52KK474 pKa = 9.94AGCTFVEE481 pKa = 4.72GDD483 pKa = 3.28GSAWDD488 pKa = 4.34TTCSTAVRR496 pKa = 11.84GMVEE500 pKa = 4.11NPILQHH506 pKa = 6.25ISRR509 pKa = 11.84ILAQTYY515 pKa = 8.84IQPQSWAEE523 pKa = 3.84AHH525 pKa = 6.9DD526 pKa = 4.12KK527 pKa = 11.37ANAAQKK533 pKa = 10.64LKK535 pKa = 10.91LYY537 pKa = 9.49FKK539 pKa = 10.35KK540 pKa = 10.53YY541 pKa = 9.38HH542 pKa = 5.32EE543 pKa = 4.5TMHH546 pKa = 7.54VEE548 pKa = 3.72ITAIRR553 pKa = 11.84RR554 pKa = 11.84SGHH557 pKa = 6.32RR558 pKa = 11.84GTSVLNWWINFTMWVCSLFDD578 pKa = 3.49KK579 pKa = 10.76PQIFLCPDD587 pKa = 3.18ARR589 pKa = 11.84WADD592 pKa = 3.61DD593 pKa = 3.48VAGVRR598 pKa = 11.84RR599 pKa = 11.84WFYY602 pKa = 11.3GVYY605 pKa = 10.3EE606 pKa = 4.78GDD608 pKa = 4.01DD609 pKa = 4.03SGASTSPKK617 pKa = 9.3LCQVSEE623 pKa = 4.41ADD625 pKa = 3.45VTALKK630 pKa = 10.19EE631 pKa = 4.21KK632 pKa = 10.5KK633 pKa = 8.54ITLAEE638 pKa = 4.37LGNKK642 pKa = 8.8YY643 pKa = 9.66RR644 pKa = 11.84VPEE647 pKa = 4.1ASVTASISALAFWDD661 pKa = 3.58RR662 pKa = 11.84AGFNMKK668 pKa = 8.79WVFARR673 pKa = 11.84KK674 pKa = 8.87RR675 pKa = 11.84GTMVGCHH682 pKa = 6.57LGLTEE687 pKa = 4.52TDD689 pKa = 3.84SEE691 pKa = 4.89SKK693 pKa = 9.45TGCVVPNGVFCPEE706 pKa = 4.49LPRR709 pKa = 11.84ALKK712 pKa = 10.7GAVSCSPAIIEE723 pKa = 4.2AVRR726 pKa = 11.84KK727 pKa = 9.94GDD729 pKa = 3.46YY730 pKa = 9.08KK731 pKa = 10.33TIKK734 pKa = 9.41TIAAAAALGRR744 pKa = 11.84AADD747 pKa = 3.92FAGKK751 pKa = 10.4VPTLSRR757 pKa = 11.84KK758 pKa = 8.85YY759 pKa = 9.85LAYY762 pKa = 10.45ANEE765 pKa = 4.45LDD767 pKa = 4.17SSDD770 pKa = 3.94FEE772 pKa = 4.45DD773 pKa = 5.26RR774 pKa = 11.84EE775 pKa = 3.96MSMRR779 pKa = 11.84TVGEE783 pKa = 4.11EE784 pKa = 4.27GMSAAAVRR792 pKa = 11.84AQIEE796 pKa = 4.28EE797 pKa = 4.2ANGAVSAAEE806 pKa = 3.71EE807 pKa = 4.32KK808 pKa = 10.39TFLEE812 pKa = 4.27ALDD815 pKa = 3.77YY816 pKa = 8.46TASVEE821 pKa = 4.08EE822 pKa = 4.34LEE824 pKa = 4.51EE825 pKa = 4.01FCRR828 pKa = 11.84YY829 pKa = 8.39PWQFDD834 pKa = 3.34AVDD837 pKa = 3.4QHH839 pKa = 8.45DD840 pKa = 4.37EE841 pKa = 4.36FACSVPAAWRR851 pKa = 11.84DD852 pKa = 3.43GASII856 pKa = 4.04

Molecular weight: 94.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KL49|A0A1L3KL49_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 3 OX=1922751 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 9.52TMRR6 pKa = 11.84AKK8 pKa = 10.5KK9 pKa = 9.8LARR12 pKa = 11.84DD13 pKa = 3.47LRR15 pKa = 11.84RR16 pKa = 11.84VKK18 pKa = 10.44RR19 pKa = 11.84DD20 pKa = 3.59LKK22 pKa = 10.88SKK24 pKa = 9.96PATRR28 pKa = 11.84RR29 pKa = 11.84NATKK33 pKa = 10.67ALAQAAMAAPRR44 pKa = 11.84RR45 pKa = 11.84NFGTKK50 pKa = 9.63SAGGTLRR57 pKa = 11.84ACIKK61 pKa = 10.72SLDD64 pKa = 3.57ARR66 pKa = 11.84VPRR69 pKa = 11.84TIGLPRR75 pKa = 11.84AVGPYY80 pKa = 9.95SVIRR84 pKa = 11.84TTRR87 pKa = 11.84LWSSSANFIMFVPFTSDD104 pKa = 3.79DD105 pKa = 3.54SWHH108 pKa = 5.74NWCGIEE114 pKa = 5.44DD115 pKa = 4.5VISSDD120 pKa = 3.97PVNGGNNSRR129 pKa = 11.84PITIPLADD137 pKa = 4.37LSHH140 pKa = 6.58AAEE143 pKa = 4.22VAPAALTVQVMNPSSVQTAQGMFAMGRR170 pKa = 11.84ANQQFDD176 pKa = 3.87YY177 pKa = 11.23GGSTEE182 pKa = 3.98TWDD185 pKa = 3.47TLRR188 pKa = 11.84DD189 pKa = 3.43RR190 pKa = 11.84FISYY194 pKa = 8.59FSPRR198 pKa = 11.84LLTGGKK204 pKa = 9.35LALRR208 pKa = 11.84GVKK211 pKa = 10.22CSTYY215 pKa = 9.6PLDD218 pKa = 3.46MSEE221 pKa = 4.78YY222 pKa = 8.54SHH224 pKa = 6.79FMPVADD230 pKa = 4.45YY231 pKa = 7.43PTPFTWLTVKK241 pKa = 9.1PTALAPIIFVQNNATPQTLEE261 pKa = 3.77FLITVEE267 pKa = 3.45WRR269 pKa = 11.84VRR271 pKa = 11.84FDD273 pKa = 3.48PGNPAVASHH282 pKa = 5.96THH284 pKa = 6.79HH285 pKa = 7.32DD286 pKa = 3.8TTPDD290 pKa = 3.69GVWNSVCRR298 pKa = 11.84EE299 pKa = 3.97MSSMGHH305 pKa = 5.21GVEE308 pKa = 4.48EE309 pKa = 4.02LAEE312 pKa = 4.14DD313 pKa = 3.98VMEE316 pKa = 4.79LGAMGGAARR325 pKa = 11.84LAATALLL332 pKa = 4.11

Molecular weight: 36.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1188

332

856

594.0

65.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.785 ± 0.02

2.02 ± 0.435

5.219 ± 0.052

6.229 ± 1.555

3.704 ± 0.113

6.566 ± 0.032

2.104 ± 0.002

3.956 ± 0.182

6.313 ± 1.439

7.492 ± 0.181

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.778 ± 0.607

2.609 ± 0.696

4.966 ± 0.885

2.525 ± 0.062

6.145 ± 0.739

7.323 ± 0.11

6.313 ± 1.131

7.407 ± 0.416

2.525 ± 0.222

2.02 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski