Wickerhamiella sorbophila

Taxonomy: cellular organisms; Eukaryota; Opisthokonta;

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4707 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0FL10|A0A2T0FL10_9ASCO Cytochrome b5 reductase 4 OS=Wickerhamiella sorbophila OX=45607 GN=B9G98_03289 PE=3 SV=1
MM1 pKa = 7.5LRR3 pKa = 11.84TIALFAGYY11 pKa = 10.54ALAQEE16 pKa = 4.26YY17 pKa = 10.24RR18 pKa = 11.84PVVPHH23 pKa = 6.34GRR25 pKa = 11.84EE26 pKa = 3.5EE27 pKa = 3.96QLRR30 pKa = 11.84QNAYY34 pKa = 9.14VDD36 pKa = 4.02VISTVVAPATQVFHH50 pKa = 6.17TTTDD54 pKa = 2.82VWVEE58 pKa = 3.84VYY60 pKa = 10.64PPVTTEE66 pKa = 3.85DD67 pKa = 3.36VTVFVPPPTHH77 pKa = 6.53TDD79 pKa = 3.51VYY81 pKa = 10.66WDD83 pKa = 4.19YY84 pKa = 12.03DD85 pKa = 3.92DD86 pKa = 6.75DD87 pKa = 7.12DD88 pKa = 6.98DD89 pKa = 6.02DD90 pKa = 5.28VYY92 pKa = 10.89EE93 pKa = 4.26TTVVVTALPGYY104 pKa = 8.55STTCEE109 pKa = 3.73TSTDD113 pKa = 4.01YY114 pKa = 11.3VDD116 pKa = 3.4KK117 pKa = 10.82TIVVPPAITSTSDD130 pKa = 2.95QSTTLPPVDD139 pKa = 3.51TTTGEE144 pKa = 4.22GSTTDD149 pKa = 3.62LTDD152 pKa = 3.18LTTFPITTATTEE164 pKa = 4.31STSEE168 pKa = 4.06SQTVHH173 pKa = 4.51TTVPSTVPTTDD184 pKa = 2.82STSGVTTEE192 pKa = 4.02STTDD196 pKa = 3.19VTTGTTTDD204 pKa = 3.47STSEE208 pKa = 4.17TTTNPATDD216 pKa = 3.83TTPSASTEE224 pKa = 4.23VEE226 pKa = 4.31TTTSNALPSTILPKK240 pKa = 10.66ASTPAPPAKK249 pKa = 9.51STPPPFTPTLPPQAEE264 pKa = 4.07NSDD267 pKa = 4.16IIPANGAYY275 pKa = 10.22NLQPLGSALYY285 pKa = 9.4FLLGIPLLFF294 pKa = 4.82

Molecular weight: 31.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0FL07|A0A2T0FL07_9ASCO 40S ribosomal protein S12 OS=Wickerhamiella sorbophila OX=45607 GN=B9G98_03280 PE=3 SV=1
MM1 pKa = 7.38SAGARR6 pKa = 11.84SAGATRR12 pKa = 11.84SDD14 pKa = 3.65RR15 pKa = 11.84PRR17 pKa = 11.84LSARR21 pKa = 11.84ARR23 pKa = 11.84SAGATRR29 pKa = 11.84SDD31 pKa = 3.65RR32 pKa = 11.84PRR34 pKa = 11.84LSARR38 pKa = 11.84ARR40 pKa = 11.84SAGATRR46 pKa = 11.84SGRR49 pKa = 11.84PRR51 pKa = 11.84LGAGARR57 pKa = 11.84SAGASRR63 pKa = 11.84SARR66 pKa = 11.84PRR68 pKa = 11.84ASARR72 pKa = 11.84SRR74 pKa = 11.84GGDD77 pKa = 3.1RR78 pKa = 11.84SRR80 pKa = 11.84LSTRR84 pKa = 11.84ARR86 pKa = 11.84NAGATRR92 pKa = 11.84NDD94 pKa = 3.41RR95 pKa = 11.84AGLNTGARR103 pKa = 11.84SAGATRR109 pKa = 11.84SDD111 pKa = 3.6RR112 pKa = 11.84PRR114 pKa = 11.84ASARR118 pKa = 11.84SRR120 pKa = 11.84GGNRR124 pKa = 11.84SRR126 pKa = 11.84LHH128 pKa = 5.45TGARR132 pKa = 11.84SAGATGNNRR141 pKa = 11.84PRR143 pKa = 11.84LDD145 pKa = 3.11ARR147 pKa = 11.84ARR149 pKa = 11.84SAGTTRR155 pKa = 11.84NDD157 pKa = 3.56RR158 pKa = 11.84AGLNAGARR166 pKa = 11.84SAGATRR172 pKa = 11.84RR173 pKa = 11.84NRR175 pKa = 11.84PRR177 pKa = 11.84PSARR181 pKa = 11.84SRR183 pKa = 11.84GGDD186 pKa = 3.1RR187 pKa = 11.84SRR189 pKa = 11.84LSTRR193 pKa = 11.84TRR195 pKa = 11.84NAGTTRR201 pKa = 11.84SNRR204 pKa = 11.84SRR206 pKa = 11.84LNAGARR212 pKa = 11.84SAGDD216 pKa = 3.47TRR218 pKa = 11.84SDD220 pKa = 3.49RR221 pKa = 11.84PP222 pKa = 3.43

Molecular weight: 23.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4707

0

4707

2105241

65

4577

447.3

49.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.524 ± 0.042

1.16 ± 0.013

5.666 ± 0.027

6.514 ± 0.037

3.913 ± 0.029

5.977 ± 0.034

2.181 ± 0.014

5.098 ± 0.025

5.355 ± 0.032

9.583 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.21 ± 0.015

3.825 ± 0.017

5.411 ± 0.038

4.169 ± 0.028

5.587 ± 0.027

7.99 ± 0.047

5.624 ± 0.042

6.978 ± 0.026

1.224 ± 0.015

3.012 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski