Streptomyces phage AbbeyMikolon

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Abbeymikolonvirus; Streptomyces virus AbbeyMikolon

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5BLA4|A0A2H5BLA4_9CAUD Uncharacterized protein OS=Streptomyces phage AbbeyMikolon OX=2059880 GN=SEA_ABBEYMIKOLON_47 PE=4 SV=1
MM1 pKa = 8.13DD2 pKa = 6.71DD3 pKa = 4.84DD4 pKa = 5.22VEE6 pKa = 4.54PCALRR11 pKa = 11.84YY12 pKa = 9.51CDD14 pKa = 5.01PDD16 pKa = 3.26MCGWSVTQRR25 pKa = 11.84TPCQWEE31 pKa = 4.2EE32 pKa = 3.99NASGEE37 pKa = 4.29PKK39 pKa = 10.6

Molecular weight: 4.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5BLF3|A0A2H5BLF3_9CAUD Uncharacterized protein OS=Streptomyces phage AbbeyMikolon OX=2059880 GN=SEA_ABBEYMIKOLON_23 PE=4 SV=1
MM1 pKa = 7.78ANPSKK6 pKa = 10.99ARR8 pKa = 11.84GTAWEE13 pKa = 4.33SSTRR17 pKa = 11.84DD18 pKa = 3.7YY19 pKa = 11.67LNEE22 pKa = 3.91ALGLYY27 pKa = 8.66WPDD30 pKa = 2.68WQEE33 pKa = 3.44RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84NGLTQWRR43 pKa = 11.84DD44 pKa = 3.05PMDD47 pKa = 4.12PANIKK52 pKa = 10.21RR53 pKa = 11.84QAQEE57 pKa = 3.76GAKK60 pKa = 10.47DD61 pKa = 3.51VGDD64 pKa = 3.38IHH66 pKa = 7.07AWPFILEE73 pKa = 4.32AKK75 pKa = 9.62DD76 pKa = 3.66VKK78 pKa = 11.06NPAVPTWMRR87 pKa = 11.84QAKK90 pKa = 10.36AEE92 pKa = 4.03AFNAGFPYY100 pKa = 10.3YY101 pKa = 10.76VVVHH105 pKa = 6.95KK106 pKa = 10.61YY107 pKa = 10.12RR108 pKa = 11.84NANVRR113 pKa = 11.84FGRR116 pKa = 11.84VHH118 pKa = 5.73MDD120 pKa = 2.29VRR122 pKa = 11.84TFTAVRR128 pKa = 11.84RR129 pKa = 11.84HH130 pKa = 5.97LGLTSRR136 pKa = 11.84QMWEE140 pKa = 4.37LYY142 pKa = 10.72SFTLSKK148 pKa = 10.28PLRR151 pKa = 11.84GLDD154 pKa = 3.13TSRR157 pKa = 11.84WYY159 pKa = 8.93FTTNLWHH166 pKa = 6.61FAALLRR172 pKa = 11.84AVRR175 pKa = 11.84AASRR179 pKa = 3.46

Molecular weight: 20.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

12679

39

1045

214.9

23.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.586 ± 0.317

0.797 ± 0.129

6.223 ± 0.225

6.357 ± 0.306

2.784 ± 0.193

8.96 ± 0.399

1.648 ± 0.198

3.415 ± 0.169

4.409 ± 0.4

8.494 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.335 ± 0.124

2.634 ± 0.133

5.237 ± 0.246

2.887 ± 0.159

7.453 ± 0.462

5.229 ± 0.253

6.878 ± 0.358

7.974 ± 0.26

1.932 ± 0.157

2.768 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski