Drosophila guanche (Fruit fly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17569 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3B0KFM7|A0A3B0KFM7_DROGU Blast:Gamma-tubulin complex component 3 OS=Drosophila guanche OX=7266 GN=DGUA_6G014315 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.19LAFIFMSVQLMLLATSLNARR22 pKa = 11.84RR23 pKa = 11.84CIRR26 pKa = 11.84PTTTTTSTPKK36 pKa = 8.49TTDD39 pKa = 3.83DD40 pKa = 3.94EE41 pKa = 4.89PSDD44 pKa = 3.97EE45 pKa = 4.75SSDD48 pKa = 3.59SSGEE52 pKa = 3.89ITNAPSDD59 pKa = 3.93PTGEE63 pKa = 4.6TTGSTPDD70 pKa = 3.69PTSKK74 pKa = 9.38PTDD77 pKa = 3.69APSDD81 pKa = 3.73PTGEE85 pKa = 4.53TTGSPSDD92 pKa = 3.69PTIEE96 pKa = 4.22PTDD99 pKa = 3.98APSDD103 pKa = 3.91PTGKK107 pKa = 9.88PSDD110 pKa = 4.37APSDD114 pKa = 3.94PPGEE118 pKa = 4.48TTGPPSDD125 pKa = 3.87ATDD128 pKa = 3.61MPTDD132 pKa = 3.8APSNPTGKK140 pKa = 8.42PTDD143 pKa = 4.09APSDD147 pKa = 3.92PPGEE151 pKa = 4.38TTNAAPDD158 pKa = 3.91PSRR161 pKa = 11.84EE162 pKa = 3.81TSAAPSDD169 pKa = 3.67PTDD172 pKa = 3.56ASGVPADD179 pKa = 4.09TTQTTTPPPAIDD191 pKa = 4.57SDD193 pKa = 4.5AACQAHH199 pKa = 6.9PGVHH203 pKa = 6.04YY204 pKa = 10.63LPNPYY209 pKa = 9.86DD210 pKa = 3.37CHH212 pKa = 8.95KK213 pKa = 10.86YY214 pKa = 9.15IQCEE218 pKa = 4.19GNVGHH223 pKa = 7.3IHH225 pKa = 6.3TCPADD230 pKa = 4.1LYY232 pKa = 9.07WNSIALTCDD241 pKa = 3.52TVCQQ245 pKa = 3.72

Molecular weight: 25.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3B0JPY2|A0A3B0JPY2_DROGU Isoform of A0A3B0JPP2 Blast:Sorbitol dehydrogenase OS=Drosophila guanche OX=7266 GN=DGUA_6G016714 PE=3 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84ADD4 pKa = 5.5LIANSNLTRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84SGSSTRR21 pKa = 11.84RR22 pKa = 11.84SSSAVGGLRR31 pKa = 11.84QRR33 pKa = 11.84LGNRR37 pKa = 11.84RR38 pKa = 11.84PSVGGASEE46 pKa = 3.83RR47 pKa = 11.84VEE49 pKa = 3.72RR50 pKa = 11.84HH51 pKa = 3.59QRR53 pKa = 11.84QLIKK57 pKa = 10.34QQQQQYY63 pKa = 10.35HH64 pKa = 5.83LQQTRR69 pKa = 11.84GRR71 pKa = 11.84SRR73 pKa = 11.84SRR75 pKa = 11.84SRR77 pKa = 11.84SRR79 pKa = 11.84LPQVNARR86 pKa = 11.84IDD88 pKa = 3.43RR89 pKa = 11.84SRR91 pKa = 11.84SQSRR95 pKa = 11.84GRR97 pKa = 11.84SAAPQFNRR105 pKa = 11.84TRR107 pKa = 11.84SQSRR111 pKa = 11.84TRR113 pKa = 11.84GGRR116 pKa = 11.84DD117 pKa = 2.92RR118 pKa = 11.84RR119 pKa = 11.84PQSGTRR125 pKa = 11.84AGQQTPRR132 pKa = 11.84VSVKK136 pKa = 9.64QRR138 pKa = 11.84LGVRR142 pKa = 11.84PGQGRR147 pKa = 11.84GGAGARR153 pKa = 11.84TRR155 pKa = 11.84AGANQAKK162 pKa = 8.27NQRR165 pKa = 11.84RR166 pKa = 11.84APSKK170 pKa = 10.39LRR172 pKa = 11.84GVAGGRR178 pKa = 11.84VEE180 pKa = 4.29KK181 pKa = 10.56RR182 pKa = 11.84RR183 pKa = 11.84NANPTQKK190 pKa = 10.52GVSASLAGRR199 pKa = 11.84RR200 pKa = 11.84GRR202 pKa = 11.84PRR204 pKa = 11.84GRR206 pKa = 11.84SVARR210 pKa = 11.84AAAAAGGGAASGSNGASTRR229 pKa = 11.84RR230 pKa = 11.84SRR232 pKa = 11.84SRR234 pKa = 11.84NRR236 pKa = 11.84PAAAAVAAAAATPGRR251 pKa = 11.84ANNTRR256 pKa = 11.84NRR258 pKa = 11.84RR259 pKa = 11.84GRR261 pKa = 11.84SAGAVKK267 pKa = 10.68GSGAKK272 pKa = 9.59GAAANKK278 pKa = 10.0NGNKK282 pKa = 10.04SNASKK287 pKa = 11.2GKK289 pKa = 7.19TNKK292 pKa = 9.21GRR294 pKa = 11.84PGGGGKK300 pKa = 9.46PGPQQQQARR309 pKa = 11.84RR310 pKa = 11.84GRR312 pKa = 11.84SRR314 pKa = 11.84TRR316 pKa = 11.84AGRR319 pKa = 11.84QAPGKK324 pKa = 10.14SVTSHH329 pKa = 6.18EE330 pKa = 4.24AHH332 pKa = 6.51SS333 pKa = 3.73

Molecular weight: 35.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13413

4156

17569

10893509

14

17223

620.0

68.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.811 ± 0.026

1.913 ± 0.027

5.114 ± 0.017

6.386 ± 0.024

3.355 ± 0.014

6.18 ± 0.023

2.73 ± 0.011

4.696 ± 0.016

5.381 ± 0.026

8.987 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.01

4.602 ± 0.013

5.611 ± 0.029

5.466 ± 0.029

5.484 ± 0.015

8.428 ± 0.027

5.824 ± 0.02

5.813 ± 0.015

0.971 ± 0.007

2.895 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski