Muribaculum intestinale

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Muribaculaceae; Muribaculum

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2553 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1S9T1|A0A1B1S9T1_9BACT Hydrolase_4 domain-containing protein OS=Muribaculum intestinale OX=1796646 GN=A4V02_07280 PE=4 SV=2
MM1 pKa = 7.33SFWNFLGEE9 pKa = 3.84VAMFNMICNLFSGKK23 pKa = 9.85SKK25 pKa = 10.51PGEE28 pKa = 3.98TSSCQSRR35 pKa = 11.84HH36 pKa = 4.66GYY38 pKa = 9.83IYY40 pKa = 10.4DD41 pKa = 3.55AEE43 pKa = 4.32YY44 pKa = 9.68EE45 pKa = 4.11ARR47 pKa = 11.84VGEE50 pKa = 4.27LEE52 pKa = 4.04HH53 pKa = 7.19EE54 pKa = 4.02IRR56 pKa = 11.84EE57 pKa = 4.35SKK59 pKa = 10.61KK60 pKa = 10.72RR61 pKa = 11.84ITEE64 pKa = 3.79YY65 pKa = 10.65QGIIDD70 pKa = 4.47NSPSFDD76 pKa = 4.95IDD78 pKa = 3.54DD79 pKa = 4.38CDD81 pKa = 4.37IDD83 pKa = 4.04EE84 pKa = 4.64LQDD87 pKa = 4.61RR88 pKa = 11.84IDD90 pKa = 3.79EE91 pKa = 4.49LEE93 pKa = 4.22SRR95 pKa = 11.84LDD97 pKa = 3.79DD98 pKa = 4.46CDD100 pKa = 4.53VMSDD104 pKa = 3.86CYY106 pKa = 11.36DD107 pKa = 4.02YY108 pKa = 11.24IQDD111 pKa = 4.78EE112 pKa = 4.31MDD114 pKa = 3.25ILQDD118 pKa = 3.75RR119 pKa = 11.84LDD121 pKa = 4.23AMQEE125 pKa = 3.85LDD127 pKa = 5.66DD128 pKa = 4.96MYY130 pKa = 11.74DD131 pKa = 5.38DD132 pKa = 4.0IPLYY136 pKa = 10.82NDD138 pKa = 3.23YY139 pKa = 11.62DD140 pKa = 5.48DD141 pKa = 5.77IDD143 pKa = 6.24RR144 pKa = 11.84DD145 pKa = 4.18DD146 pKa = 3.84DD147 pKa = 3.61WW148 pKa = 6.6

Molecular weight: 17.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1S866|A0A1B1S866_9BACT Class A beta-lactamase OS=Muribaculum intestinale OX=1796646 GN=A4V02_04180 PE=4 SV=1
MM1 pKa = 7.75FDD3 pKa = 4.33FIVAISVEE11 pKa = 4.71FYY13 pKa = 10.48KK14 pKa = 11.04VSSQKK19 pKa = 10.53IIIRR23 pKa = 11.84AVARR27 pKa = 11.84LITQLTPRR35 pKa = 11.84NIGRR39 pKa = 11.84RR40 pKa = 11.84NNHH43 pKa = 5.07NTNPSIAPIIPTNQRR58 pKa = 11.84ITLFMRR64 pKa = 11.84LRR66 pKa = 11.84LEE68 pKa = 3.78MDD70 pKa = 4.06ALPRR74 pKa = 11.84LFDD77 pKa = 3.18MLLFVFIVRR86 pKa = 11.84HH87 pKa = 5.47FFYY90 pKa = 10.86ASNRR94 pKa = 11.84LSKK97 pKa = 10.78FCLLVDD103 pKa = 4.18TVACGGKK110 pKa = 9.97EE111 pKa = 3.92GLFALL116 pKa = 4.95

Molecular weight: 13.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2553

0

2553

928564

38

2476

363.7

40.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.321 ± 0.057

1.258 ± 0.018

6.202 ± 0.033

5.814 ± 0.044

4.129 ± 0.029

7.211 ± 0.045

1.993 ± 0.021

6.831 ± 0.042

5.02 ± 0.042

8.529 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.928 ± 0.018

4.581 ± 0.037

4.223 ± 0.026

2.8 ± 0.024

5.624 ± 0.041

6.563 ± 0.035

5.825 ± 0.037

6.796 ± 0.038

1.308 ± 0.022

4.042 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski