Candidatus Nucleicultrix amoebiphila FS5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales; Caedimonadaceae; Candidatus Nucleicultrix; Candidatus Nucleicultrix amoebiphila

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1448 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6N5S4|A0A1W6N5S4_9PROT Glycine--tRNA ligase beta subunit OS=Candidatus Nucleicultrix amoebiphila FS5 OX=1414854 GN=glyS PE=3 SV=1
MM1 pKa = 7.67AKK3 pKa = 8.41ITFIKK8 pKa = 10.56QNGEE12 pKa = 4.03QIQIDD17 pKa = 4.28APLGLSVLEE26 pKa = 4.52IAHH29 pKa = 5.87QNNIDD34 pKa = 4.41LEE36 pKa = 4.78GACEE40 pKa = 3.97GSLACSTCHH49 pKa = 6.5VIVGEE54 pKa = 4.02DD55 pKa = 3.29WYY57 pKa = 10.68DD58 pKa = 3.52VLSPATEE65 pKa = 4.48DD66 pKa = 3.68EE67 pKa = 4.24EE68 pKa = 6.48DD69 pKa = 3.71MLDD72 pKa = 3.47LAFGLTHH79 pKa = 6.37TSRR82 pKa = 11.84LGCQIIMTDD91 pKa = 3.55EE92 pKa = 4.38LDD94 pKa = 3.78GLVVKK99 pKa = 10.45LPAATRR105 pKa = 11.84NMMVEE110 pKa = 4.16SS111 pKa = 3.93

Molecular weight: 12.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6N5X6|A0A1W6N5X6_9PROT Uncharacterized protein OS=Candidatus Nucleicultrix amoebiphila FS5 OX=1414854 GN=GQ61_08035 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.71QPKK8 pKa = 9.16NLIRR12 pKa = 11.84KK13 pKa = 8.33RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 5.11GFLSRR21 pKa = 11.84MQTPGGRR28 pKa = 11.84RR29 pKa = 11.84VIANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1448

0

1448

446774

41

2806

308.5

34.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.295 ± 0.07

0.87 ± 0.019

4.975 ± 0.055

6.45 ± 0.068

4.795 ± 0.055

6.448 ± 0.07

2.35 ± 0.034

7.239 ± 0.061

7.053 ± 0.067

10.839 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.414 ± 0.028

4.051 ± 0.043

4.188 ± 0.038

3.885 ± 0.044

4.607 ± 0.045

6.818 ± 0.053

5.386 ± 0.043

6.249 ± 0.058

1.071 ± 0.026

3.017 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski