Hubei unio douglasiae virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGI3|A0A1L3KGI3_9VIRU RNA replicase OS=Hubei unio douglasiae virus 3 OX=1923323 PE=3 SV=1
MM1 pKa = 7.39AWWALAYY8 pKa = 9.6PLTQCMLGLWTSIALWQWVAFTRR31 pKa = 11.84APHH34 pKa = 6.65AYY36 pKa = 8.81AVAWGWLPEE45 pKa = 4.2CQSTGVWPWSRR56 pKa = 11.84DD57 pKa = 3.39TMLDD61 pKa = 3.2AAHH64 pKa = 5.75RR65 pKa = 11.84QVWRR69 pKa = 11.84FARR72 pKa = 11.84ARR74 pKa = 11.84GLCVEE79 pKa = 5.01PQHH82 pKa = 5.96SWWWLVIPPLCLILGYY98 pKa = 9.18TGARR102 pKa = 11.84CVRR105 pKa = 11.84YY106 pKa = 9.5GVLAVWFEE114 pKa = 4.52GVRR117 pKa = 11.84TLHH120 pKa = 6.6PKK122 pKa = 10.54ADD124 pKa = 3.93TKK126 pKa = 11.04RR127 pKa = 11.84KK128 pKa = 9.79LSGVKK133 pKa = 10.07CPPRR137 pKa = 11.84VATNPANPHH146 pKa = 5.67AAGAAIRR153 pKa = 11.84LQVHH157 pKa = 6.16KK158 pKa = 9.86FVHH161 pKa = 6.89AICAAWDD168 pKa = 3.44TGATVVRR175 pKa = 11.84YY176 pKa = 9.4DD177 pKa = 3.37LSYY180 pKa = 11.43AKK182 pKa = 9.74RR183 pKa = 11.84DD184 pKa = 3.66EE185 pKa = 4.25MFGTSVLGRR194 pKa = 11.84RR195 pKa = 11.84WLRR198 pKa = 11.84SAQDD202 pKa = 3.18LSQPVRR208 pKa = 11.84DD209 pKa = 4.24DD210 pKa = 3.46PRR212 pKa = 11.84PIGVPVSAIDD222 pKa = 3.14TAYY225 pKa = 11.13YY226 pKa = 9.73LRR228 pKa = 11.84PYY230 pKa = 10.62DD231 pKa = 3.38WDD233 pKa = 3.82EE234 pKa = 4.2FCGEE238 pKa = 4.59LIVFNAQLPTAIAWKK253 pKa = 9.95EE254 pKa = 4.04SWTTAYY260 pKa = 10.64FDD262 pKa = 3.88AQSVLHH268 pKa = 5.71EE269 pKa = 4.35QIAGGKK275 pKa = 7.72TYY277 pKa = 7.52THH279 pKa = 7.42PLPEE283 pKa = 5.31PGLHH287 pKa = 6.18EE288 pKa = 5.17LFLHH292 pKa = 5.48TRR294 pKa = 11.84GWFWNQRR301 pKa = 11.84VVCYY305 pKa = 8.67EE306 pKa = 3.74QTVVRR311 pKa = 11.84VPDD314 pKa = 3.26SRR316 pKa = 11.84QAIFIWTPQYY326 pKa = 10.94TITAPWWAFCAMYY339 pKa = 10.62RR340 pKa = 11.84FMTGSVPPRR349 pKa = 11.84PRR351 pKa = 11.84NWEE354 pKa = 3.46GAVRR358 pKa = 11.84VEE360 pKa = 4.74SSAGDD365 pKa = 3.26RR366 pKa = 11.84KK367 pKa = 10.5VSVVQMVTPDD377 pKa = 3.44GKK379 pKa = 10.36ISARR383 pKa = 11.84LEE385 pKa = 3.9STIDD389 pKa = 3.22GRR391 pKa = 11.84AFEE394 pKa = 4.52LDD396 pKa = 3.03RR397 pKa = 11.84TFVEE401 pKa = 5.26TVALRR406 pKa = 11.84GAVHH410 pKa = 6.78GSPVSIHH417 pKa = 6.66DD418 pKa = 3.68TSSMANRR425 pKa = 11.84MEE427 pKa = 4.47GVSTDD432 pKa = 3.56PAAVTMSAVVAGAGVLPVTTTPVGYY457 pKa = 10.54VPGAGVEE464 pKa = 4.26TAGARR469 pKa = 11.84VPTKK473 pKa = 10.58EE474 pKa = 3.84EE475 pKa = 4.59GIAGLVNPPASVAAHH490 pKa = 6.11TPAAVAAVLGRR501 pKa = 11.84VEE503 pKa = 4.09QLQVAVPRR511 pKa = 11.84TISPALKK518 pKa = 10.12SEE520 pKa = 3.89LMAAARR526 pKa = 11.84YY527 pKa = 9.43LGGLIGPMDD536 pKa = 4.28PKK538 pKa = 10.77SRR540 pKa = 11.84EE541 pKa = 3.85FVLEE545 pKa = 4.13SMTKK549 pKa = 8.42PAQRR553 pKa = 11.84ARR555 pKa = 11.84NAKK558 pKa = 10.04LPQQDD563 pKa = 3.73VAVMPDD569 pKa = 3.7AKK571 pKa = 10.28PSTMQKK577 pKa = 8.99TEE579 pKa = 4.09ATPKK583 pKa = 9.81PNPRR587 pKa = 11.84VINIVEE593 pKa = 3.83TDD595 pKa = 3.72FNVEE599 pKa = 3.68YY600 pKa = 11.28GRR602 pKa = 11.84FTKK605 pKa = 10.61AFYY608 pKa = 10.71AGLKK612 pKa = 8.77KK613 pKa = 10.76LPMCGSGVDD622 pKa = 3.81ATALAAKK629 pKa = 9.96IADD632 pKa = 3.78STVAGLLDD640 pKa = 4.61SIDD643 pKa = 3.72IADD646 pKa = 3.88MDD648 pKa = 4.34ASQGTTARR656 pKa = 11.84VDD658 pKa = 2.9IYY660 pKa = 10.5EE661 pKa = 4.07EE662 pKa = 3.98VMRR665 pKa = 11.84TVFAAHH671 pKa = 5.74LQEE674 pKa = 5.67LEE676 pKa = 4.15PLLLAARR683 pKa = 11.84TGKK686 pKa = 8.4LTYY689 pKa = 9.99RR690 pKa = 11.84SRR692 pKa = 11.84VDD694 pKa = 3.48DD695 pKa = 4.19AEE697 pKa = 4.42KK698 pKa = 10.06MVAVVSDD705 pKa = 4.51PNVSGCSDD713 pKa = 3.43TTNVNTIIMIVVALVAAQRR732 pKa = 11.84TDD734 pKa = 2.72GGYY737 pKa = 7.79DD738 pKa = 3.16THH740 pKa = 7.02PCGLFSGDD748 pKa = 4.35DD749 pKa = 3.46GLVPKK754 pKa = 10.37RR755 pKa = 11.84IRR757 pKa = 11.84SAFDD761 pKa = 2.92AVMAEE766 pKa = 4.04LGFTVKK772 pKa = 10.5VEE774 pKa = 4.32DD775 pKa = 3.96LASPEE780 pKa = 3.91AVKK783 pKa = 10.33FLNQIFVSPSTTRR796 pKa = 11.84TSTPSMHH803 pKa = 7.09RR804 pKa = 11.84LLSKK808 pKa = 10.74LCVITTANAGLLEE821 pKa = 5.1KK822 pKa = 10.76IMGLNISYY830 pKa = 9.29PRR832 pKa = 11.84NPIVQAVTRR841 pKa = 11.84AYY843 pKa = 9.12TNAYY847 pKa = 8.59GLAGADD853 pKa = 3.62MQRR856 pKa = 11.84EE857 pKa = 4.02RR858 pKa = 11.84ARR860 pKa = 11.84LKK862 pKa = 10.5KK863 pKa = 10.47RR864 pKa = 11.84PTYY867 pKa = 10.33EE868 pKa = 3.62LAAKK872 pKa = 10.07QEE874 pKa = 4.34AGAFLYY880 pKa = 10.47DD881 pKa = 3.98SKK883 pKa = 11.82DD884 pKa = 3.15EE885 pKa = 4.16AAVYY889 pKa = 10.49RR890 pKa = 11.84EE891 pKa = 4.01IAHH894 pKa = 6.43EE895 pKa = 4.31AGKK898 pKa = 8.94DD899 pKa = 3.8TAWVRR904 pKa = 11.84AWVKK908 pKa = 10.21EE909 pKa = 3.93LGKK912 pKa = 10.59VRR914 pKa = 11.84TQDD917 pKa = 3.74DD918 pKa = 3.94LLKK921 pKa = 11.11VGFLRR926 pKa = 11.84DD927 pKa = 3.9DD928 pKa = 3.86GSEE931 pKa = 4.0VDD933 pKa = 3.8GKK935 pKa = 11.26YY936 pKa = 10.35PITRR940 pKa = 11.84VAYY943 pKa = 8.45PAVPGKK949 pKa = 10.27GKK951 pKa = 10.63RR952 pKa = 11.84PGSSS956 pKa = 2.67

Molecular weight: 104.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGI3|A0A1L3KGI3_9VIRU RNA replicase OS=Hubei unio douglasiae virus 3 OX=1923323 PE=3 SV=1
MM1 pKa = 7.53PPKK4 pKa = 10.2AAEE7 pKa = 3.78QRR9 pKa = 11.84IVVAVAKK16 pKa = 10.31GVSQGAANAAKK27 pKa = 10.0QSKK30 pKa = 9.02ARR32 pKa = 11.84KK33 pKa = 8.4GGKK36 pKa = 8.28AQRR39 pKa = 11.84QRR41 pKa = 11.84MSIVPRR47 pKa = 11.84SVRR50 pKa = 11.84QLDD53 pKa = 3.95PPAMAWLKK61 pKa = 9.74MLNDD65 pKa = 3.64PCYY68 pKa = 10.78GRR70 pKa = 11.84LSHH73 pKa = 6.72PVYY76 pKa = 10.36PGADD80 pKa = 2.97GGYY83 pKa = 9.48LSRR86 pKa = 11.84FEE88 pKa = 4.76SEE90 pKa = 3.93ATFVAGVGTTAGIIGFVPGVLPFAVVGSGVVSDD123 pKa = 3.74ITASALADD131 pKa = 4.12LSAASAPGYY140 pKa = 9.18TYY142 pKa = 11.12LRR144 pKa = 11.84GVANSVRR151 pKa = 11.84CVSACMQVSWPGSEE165 pKa = 4.64LNRR168 pKa = 11.84QGFVTLGQSTGAVLAEE184 pKa = 3.8AAAAFGNTPVTPASLRR200 pKa = 11.84PLCHH204 pKa = 6.53LRR206 pKa = 11.84TRR208 pKa = 11.84MPEE211 pKa = 3.71TVAEE215 pKa = 4.56VKK217 pKa = 9.12WRR219 pKa = 11.84PTLADD224 pKa = 4.08AQWHH228 pKa = 6.97DD229 pKa = 4.05PLVAPSYY236 pKa = 10.57GRR238 pKa = 11.84VNEE241 pKa = 4.26AGALLATFGNLPLDD255 pKa = 3.75VNGNGIGVRR264 pKa = 11.84VRR266 pKa = 11.84FIVTYY271 pKa = 9.1EE272 pKa = 4.18WIPRR276 pKa = 11.84GAQGLTSGFDD286 pKa = 3.24DD287 pKa = 4.33RR288 pKa = 11.84ARR290 pKa = 11.84SSNTLDD296 pKa = 4.7DD297 pKa = 4.85VINTLDD303 pKa = 3.52RR304 pKa = 11.84NGPEE308 pKa = 3.36WAYY311 pKa = 9.83TIGRR315 pKa = 11.84ATSVLGMMGSAYY327 pKa = 10.43VAGRR331 pKa = 11.84RR332 pKa = 11.84NRR334 pKa = 11.84LMGG337 pKa = 4.01

Molecular weight: 35.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1293

337

956

646.5

70.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.684 ± 0.283

1.392 ± 0.087

4.872 ± 0.429

4.176 ± 0.512

2.784 ± 0.048

7.966 ± 1.275

1.624 ± 0.311

3.558 ± 0.25

3.712 ± 0.566

7.579 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.552 ± 0.05

2.475 ± 0.585

6.574 ± 0.019

3.171 ± 0.039

7.038 ± 0.287

5.878 ± 0.526

6.806 ± 0.494

9.667 ± 0.053

2.552 ± 0.327

2.939 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski