Bacillus sp. UMB0899

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5279 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N6RIZ8|A0A2N6RIZ8_9BACI Uncharacterized protein OS=Bacillus sp. UMB0899 OX=2029108 GN=CJ195_10980 PE=4 SV=1
MM1 pKa = 7.64AKK3 pKa = 8.89YY4 pKa = 9.46TMVDD8 pKa = 2.97KK9 pKa = 7.78EE10 pKa = 4.42TCIACGACGATAPDD24 pKa = 3.77LFDD27 pKa = 4.87YY28 pKa = 10.76DD29 pKa = 4.34DD30 pKa = 4.22EE31 pKa = 6.89GIAEE35 pKa = 5.4AILDD39 pKa = 3.88NNQGVTAVSEE49 pKa = 4.2DD50 pKa = 4.1FEE52 pKa = 7.47DD53 pKa = 4.32DD54 pKa = 5.08LIDD57 pKa = 4.73AEE59 pKa = 4.72EE60 pKa = 4.75GCPTEE65 pKa = 4.39SIKK68 pKa = 10.86VSNQPFHH75 pKa = 5.65THH77 pKa = 6.8AEE79 pKa = 4.21VV80 pKa = 3.02

Molecular weight: 8.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N6RC36|A0A2N6RC36_9BACI Fructosamine deglycase OS=Bacillus sp. UMB0899 OX=2029108 GN=CJ195_18020 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.48VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.94VLSAA44 pKa = 4.11

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5279

0

5279

1537910

26

2046

291.3

32.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.241 ± 0.034

0.738 ± 0.01

5.109 ± 0.025

7.619 ± 0.039

4.645 ± 0.03

6.539 ± 0.031

2.132 ± 0.016

8.246 ± 0.038

7.194 ± 0.031

9.782 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.666 ± 0.015

4.801 ± 0.023

3.454 ± 0.021

3.785 ± 0.024

3.709 ± 0.021

6.306 ± 0.025

5.464 ± 0.024

6.859 ± 0.029

1.039 ± 0.013

3.671 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski