Aeromonas phage CF8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 244 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4Y5TLU2|A0A4Y5TLU2_9CAUD Uncharacterized protein OS=Aeromonas phage CF8 OX=2575324 GN=CF8_0120 PE=4 SV=1
MM1 pKa = 7.87GINTDD6 pKa = 2.84IVVYY10 pKa = 10.01KK11 pKa = 10.3FNSIDD16 pKa = 3.6LEE18 pKa = 4.64DD19 pKa = 4.63YY20 pKa = 11.01VEE22 pKa = 4.5GWNEE26 pKa = 3.85NQITLALEE34 pKa = 4.29GALNLIFHH42 pKa = 7.12PSVTSNTEE50 pKa = 3.39IYY52 pKa = 10.58AGEE55 pKa = 4.01PWSEE59 pKa = 3.91IEE61 pKa = 4.05NQIVTEE67 pKa = 4.19TVNLIQSHH75 pKa = 6.1SDD77 pKa = 2.82GSEE80 pKa = 3.5FGFRR84 pKa = 11.84LFDD87 pKa = 3.57TEE89 pKa = 4.23EE90 pKa = 4.1EE91 pKa = 4.17EE92 pKa = 4.83EE93 pKa = 4.21RR94 pKa = 11.84WDD96 pKa = 5.03LLFLEE101 pKa = 4.35QTNNIEE107 pKa = 4.11AQFGSLLEE115 pKa = 4.35KK116 pKa = 10.38LAEE119 pKa = 5.32DD120 pKa = 4.25ISSDD124 pKa = 3.72HH125 pKa = 6.54YY126 pKa = 11.7NPDD129 pKa = 2.91VNTLYY134 pKa = 11.46GEE136 pKa = 4.31MMLSAKK142 pKa = 10.43LILRR146 pKa = 11.84SYY148 pKa = 11.08AYY150 pKa = 9.34YY151 pKa = 10.36TDD153 pKa = 3.54TGVLRR158 pKa = 11.84LAIQCQQ164 pKa = 3.23

Molecular weight:
18.77 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4Y5TP62|A0A4Y5TP62_9CAUD Uncharacterized protein OS=Aeromonas phage CF8 OX=2575324 GN=CF8_0212 PE=4 SV=1
MM1 pKa = 7.68FKK3 pKa = 10.76NLWFWLRR10 pKa = 11.84VRR12 pKa = 11.84WLIVYY17 pKa = 8.76FNLWQFKK24 pKa = 9.96EE25 pKa = 4.2RR26 pKa = 11.84VLNACFCPKK35 pKa = 9.89RR36 pKa = 11.84NHH38 pKa = 6.16VLTQGHH44 pKa = 7.32IINLRR49 pKa = 11.84MGNDD53 pKa = 3.48VILIRR58 pKa = 11.84HH59 pKa = 4.93CTWWFGRR66 pKa = 11.84QGFKK70 pKa = 10.94LIGVKK75 pKa = 10.53YY76 pKa = 9.01PVNSGIRR83 pKa = 11.84LVGQDD88 pKa = 2.72VCLRR92 pKa = 11.84LKK94 pKa = 10.66PNEE97 pKa = 4.12TASQAVIRR105 pKa = 11.84YY106 pKa = 6.76NATVRR111 pKa = 11.84KK112 pKa = 9.2VHH114 pKa = 7.07DD115 pKa = 3.89AAEE118 pKa = 4.28QINQITKK125 pKa = 9.6EE126 pKa = 4.25LNSKK130 pKa = 10.59SPFIKK135 pKa = 10.53AA136 pKa = 3.28

Molecular weight:
16.08 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

244

0

244

73531

39

2354

301.4

34.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.86 ± 0.184

0.998 ± 0.06

6.214 ± 0.083

6.512 ± 0.149

4.769 ± 0.08

6.567 ± 0.17

1.972 ± 0.075

6.54 ± 0.112

6.56 ± 0.155

8.939 ± 0.143

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.064

5.636 ± 0.143

3.985 ± 0.097

3.88 ± 0.093

4.455 ± 0.092

6.299 ± 0.111

6.019 ± 0.127

6.925 ± 0.14

1.171 ± 0.055

4.053 ± 0.116

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski