Equine arteritis virus (strain Bucyrus) (EAV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Arnidovirineae; Arteriviridae; Equarterivirinae; Alphaarterivirus; Alphaarterivirus equid; Equine arteritis virus

Average proteome isoelectric point is 7.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q91DM1|E_EAVBU Envelope small membrane protein OS=Equine arteritis virus (strain Bucyrus) OX=299386 GN=GP2a PE=1 SV=1
MM1 pKa = 7.93LSMIVLLFLLWGAPSHH17 pKa = 6.92AYY19 pKa = 9.84FSYY22 pKa = 9.49YY23 pKa = 8.13TAQRR27 pKa = 11.84FTDD30 pKa = 4.11FTLCMLTDD38 pKa = 3.67RR39 pKa = 11.84GVIANLLRR47 pKa = 11.84YY48 pKa = 9.57DD49 pKa = 3.76EE50 pKa = 4.59HH51 pKa = 6.15TALYY55 pKa = 9.8NCSASKK61 pKa = 8.89TCWYY65 pKa = 8.61CTFLDD70 pKa = 3.81EE71 pKa = 5.32QIITFGTDD79 pKa = 2.76CDD81 pKa = 3.58DD82 pKa = 3.88TYY84 pKa = 11.63AVPVAEE90 pKa = 4.74VLEE93 pKa = 4.49QAHH96 pKa = 6.3GPYY99 pKa = 9.99SALFDD104 pKa = 4.38DD105 pKa = 5.07MPPFIYY111 pKa = 10.25YY112 pKa = 10.11GRR114 pKa = 11.84EE115 pKa = 3.52FGIVVLDD122 pKa = 3.16VFMFYY127 pKa = 10.43PVLVLFFLSVLPYY140 pKa = 9.36ATLILEE146 pKa = 4.33MCVSILFIIYY156 pKa = 9.84GIYY159 pKa = 10.04SGAYY163 pKa = 7.89LAMGIFAATLAIHH176 pKa = 6.54SIVVLRR182 pKa = 11.84QLLWLCLAWRR192 pKa = 11.84YY193 pKa = 10.1RR194 pKa = 11.84CTLHH198 pKa = 7.36ASFISAEE205 pKa = 4.12GKK207 pKa = 10.25VYY209 pKa = 10.23PVDD212 pKa = 4.17PGLPVAAVGNRR223 pKa = 11.84LLVPGRR229 pKa = 11.84PTIDD233 pKa = 3.26YY234 pKa = 10.46AVAYY238 pKa = 8.79GSKK241 pKa = 10.62VNLVRR246 pKa = 11.84LGAAEE251 pKa = 3.88VWEE254 pKa = 4.33PP255 pKa = 3.68

Molecular weight: 28.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P19811-2|RPOA-2_EAVBU Isoform of P19811 Isoform Replicase polyprotein 1a of Replicase polyprotein 1ab OS=Equine arteritis virus (strain Bucyrus) OX=299386 GN=rep PE=1 SV=3
MM1 pKa = 7.22GLVWSLISNSIQTIIADD18 pKa = 3.94FAISVIDD25 pKa = 3.5AALFFLMLLALAVVTVFLFWLIVAIGRR52 pKa = 11.84SLVARR57 pKa = 11.84CSRR60 pKa = 11.84GARR63 pKa = 11.84YY64 pKa = 9.51RR65 pKa = 11.84PVV67 pKa = 2.74

Molecular weight: 7.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

6097

59

3175

609.7

66.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.578 ± 0.268

3.379 ± 0.306

4.658 ± 0.497

3.182 ± 0.533

4.478 ± 0.699

7.43 ± 0.724

2.263 ± 0.408

3.789 ± 0.781

3.477 ± 0.739

11.055 ± 1.01

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.919 ± 0.291

2.788 ± 0.185

5.872 ± 0.47

2.952 ± 0.245

5.363 ± 0.403

7.118 ± 0.484

6.282 ± 0.452

8.988 ± 0.465

1.673 ± 0.159

3.756 ± 0.543
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski