Prevotella sp. CAG:755

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1936 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5GGU1|R5GGU1_9BACT DEAD/DEAH box helicase OS=Prevotella sp. CAG:755 OX=1262935 GN=BN773_00927 PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 10.21KK3 pKa = 10.35YY4 pKa = 10.09ICNVCEE10 pKa = 4.48WIYY13 pKa = 11.43DD14 pKa = 3.89PALGDD19 pKa = 3.87PDD21 pKa = 5.24SGIKK25 pKa = 10.09PGTAFEE31 pKa = 5.85DD32 pKa = 4.04IPDD35 pKa = 3.7DD36 pKa = 3.88WVCPVCGVGKK46 pKa = 10.43EE47 pKa = 4.16DD48 pKa = 4.38FSPVEE53 pKa = 4.12DD54 pKa = 3.58

Molecular weight: 5.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5GV56|R5GV56_9BACT Uncharacterized protein OS=Prevotella sp. CAG:755 OX=1262935 GN=BN773_00712 PE=4 SV=1
MM1 pKa = 7.11VRR3 pKa = 11.84LSGASGARR11 pKa = 11.84RR12 pKa = 11.84DD13 pKa = 3.83RR14 pKa = 11.84RR15 pKa = 11.84TATPRR20 pKa = 11.84RR21 pKa = 11.84GHH23 pKa = 7.32LLPPHH28 pKa = 6.76HH29 pKa = 7.08PRR31 pKa = 11.84HH32 pKa = 5.62RR33 pKa = 11.84HH34 pKa = 4.41PLRR37 pKa = 11.84PRR39 pKa = 11.84HH40 pKa = 5.65RR41 pKa = 11.84ARR43 pKa = 11.84LQGFAPRR50 pKa = 11.84DD51 pKa = 3.57RR52 pKa = 11.84RR53 pKa = 11.84GHH55 pKa = 5.79AGHH58 pKa = 6.88RR59 pKa = 11.84RR60 pKa = 11.84LPVEE64 pKa = 3.41QHH66 pKa = 4.38AHH68 pKa = 4.78HH69 pKa = 6.92RR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84HH74 pKa = 5.62DD75 pKa = 3.47SEE77 pKa = 5.22IPVLDD82 pKa = 3.21VRR84 pKa = 11.84GRR86 pKa = 11.84PRR88 pKa = 11.84RR89 pKa = 11.84IRR91 pKa = 11.84QVRR94 pKa = 11.84PRR96 pKa = 11.84QQGMARR102 pKa = 11.84RR103 pKa = 11.84LSQPHH108 pKa = 6.45RR109 pKa = 11.84DD110 pKa = 2.96KK111 pKa = 11.34HH112 pKa = 4.8RR113 pKa = 11.84TEE115 pKa = 3.78RR116 pKa = 11.84TLDD119 pKa = 3.62VRR121 pKa = 11.84LHH123 pKa = 6.4RR124 pKa = 11.84APLQFQPRR132 pKa = 11.84RR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84QLGQRR140 pKa = 11.84RR141 pKa = 11.84RR142 pKa = 11.84HH143 pKa = 4.62QLVRR147 pKa = 11.84HH148 pKa = 4.73QQRR151 pKa = 11.84PTRR154 pKa = 11.84RR155 pKa = 11.84PLLQLGRR162 pKa = 11.84KK163 pKa = 7.03RR164 pKa = 11.84TEE166 pKa = 3.84NGHH169 pKa = 5.28QHH171 pKa = 6.32LQQALRR177 pKa = 11.84HH178 pKa = 5.9LGRR181 pKa = 11.84TLQPRR186 pKa = 11.84AGGQQRR192 pKa = 11.84HH193 pKa = 5.62RR194 pKa = 11.84LRR196 pKa = 11.84HH197 pKa = 4.85HH198 pKa = 6.12PQRR201 pKa = 11.84NSHH204 pKa = 5.93LPHH207 pKa = 7.08RR208 pKa = 11.84EE209 pKa = 3.91HH210 pKa = 7.28KK211 pKa = 9.88PLPDD215 pKa = 2.94RR216 pKa = 11.84RR217 pKa = 11.84TPGRR221 pKa = 11.84QQ222 pKa = 2.85

Molecular weight: 26.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1936

0

1936

642100

29

1883

331.7

36.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.28 ± 0.07

1.41 ± 0.023

5.939 ± 0.041

5.955 ± 0.053

4.242 ± 0.033

7.477 ± 0.048

2.283 ± 0.028

5.242 ± 0.051

4.473 ± 0.055

9.446 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.563 ± 0.027

3.73 ± 0.048

4.301 ± 0.034

3.233 ± 0.031

6.629 ± 0.06

5.525 ± 0.04

5.954 ± 0.05

7.352 ± 0.046

1.201 ± 0.025

3.762 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski