Lelliottia phage phD2B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Molineuxvirinae; Tuodvirus; Lelliottia virus phD2B

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088FWN3|A0A088FWN3_9CAUD Putative scaffolding protein OS=Lelliottia phage phD2B OX=1542498 GN=phD2B_0031 PE=4 SV=1
MM1 pKa = 6.66STKK4 pKa = 10.67LEE6 pKa = 3.87TAARR10 pKa = 11.84SVQVAEE16 pKa = 5.22AIFEE20 pKa = 4.13GLSCGIEE27 pKa = 3.68PCYY30 pKa = 11.03NLLQEE35 pKa = 4.43AHH37 pKa = 7.26DD38 pKa = 4.82LGLSVEE44 pKa = 4.84AIRR47 pKa = 11.84EE48 pKa = 4.2KK49 pKa = 10.69VQEE52 pKa = 4.35LYY54 pKa = 11.26GEE56 pKa = 4.96DD57 pKa = 3.7EE58 pKa = 5.74DD59 pKa = 4.49EE60 pKa = 4.43TTDD63 pKa = 3.41

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088FT58|A0A088FT58_9CAUD Uncharacterized protein OS=Lelliottia phage phD2B OX=1542498 GN=phD2B_0019 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.27RR3 pKa = 11.84NQVKK7 pKa = 9.87QSHH10 pKa = 6.42LFRR13 pKa = 11.84TLRR16 pKa = 11.84EE17 pKa = 4.15CGVGEE22 pKa = 4.27RR23 pKa = 11.84KK24 pKa = 9.32ALRR27 pKa = 11.84MIKK30 pKa = 10.08HH31 pKa = 5.88AMFVNKK37 pKa = 10.06KK38 pKa = 10.47AGDD41 pKa = 3.75NNYY44 pKa = 9.95DD45 pKa = 3.51CSPAGFCIWVDD56 pKa = 3.06QGKK59 pKa = 8.84YY60 pKa = 9.32ARR62 pKa = 11.84TWQGVGHH69 pKa = 6.83ARR71 pKa = 3.65

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

13573

39

1260

277.0

30.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.578 ± 0.615

1.009 ± 0.173

5.828 ± 0.19

6.601 ± 0.299

3.853 ± 0.199

8.406 ± 0.316

2.254 ± 0.254

5.069 ± 0.263

6.233 ± 0.31

7.397 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.249 ± 0.219

4.421 ± 0.24

3.477 ± 0.209

3.986 ± 0.246

5.533 ± 0.28

5.931 ± 0.227

5.835 ± 0.399

7.073 ± 0.289

1.304 ± 0.125

2.962 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski