Bacillus smithii

Taxonomy: cellular organisms; Bacteria;

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3481 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4PLR9|A0A0H4PLR9_9BACI 30S ribosomal protein S18 OS=Bacillus smithii OX=1479 GN=rpsR PE=3 SV=1
MM1 pKa = 7.36EE2 pKa = 6.34HH3 pKa = 7.15GDD5 pKa = 3.4QHH7 pKa = 5.54ITVVDD12 pKa = 3.67EE13 pKa = 4.41EE14 pKa = 4.76GNEE17 pKa = 3.96ILCEE21 pKa = 3.95VLFTFDD27 pKa = 4.97SEE29 pKa = 4.63QFDD32 pKa = 3.43KK33 pKa = 11.25SYY35 pKa = 11.33VLYY38 pKa = 10.79YY39 pKa = 9.97PAEE42 pKa = 4.17TTDD45 pKa = 3.7DD46 pKa = 3.79EE47 pKa = 4.76EE48 pKa = 5.45IEE50 pKa = 3.89IFAASFHH57 pKa = 6.36PSEE60 pKa = 4.82GNSGDD65 pKa = 3.84LEE67 pKa = 4.17PVEE70 pKa = 5.13TEE72 pKa = 4.31EE73 pKa = 4.0EE74 pKa = 3.76WDD76 pKa = 4.78LIEE79 pKa = 5.3EE80 pKa = 4.2MLNTFLDD87 pKa = 3.58QEE89 pKa = 4.5EE90 pKa = 4.39EE91 pKa = 4.23HH92 pKa = 6.31EE93 pKa = 4.54HH94 pKa = 5.02NHH96 pKa = 4.36EE97 pKa = 4.42HH98 pKa = 5.24EE99 pKa = 4.35HH100 pKa = 5.94EE101 pKa = 4.32KK102 pKa = 11.08

Molecular weight: 12.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4NUK7|A0A0H4NUK7_9BACI Ribosome-associated heat shock protein implicated in the recycling of the 50S subunit OS=Bacillus smithii OX=1479 GN=BSM4216_0067 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.3QPNKK9 pKa = 8.15RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84SKK14 pKa = 9.96VHH16 pKa = 5.92GFRR19 pKa = 11.84KK20 pKa = 10.02RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.37VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.59GRR39 pKa = 11.84KK40 pKa = 8.44VLSAA44 pKa = 4.05

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3481

0

3481

854256

29

1538

245.4

27.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.926 ± 0.039

0.793 ± 0.014

4.809 ± 0.037

7.443 ± 0.055

4.688 ± 0.04

6.76 ± 0.042

2.296 ± 0.024

7.724 ± 0.049

7.516 ± 0.04

9.78 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.787 ± 0.023

4.056 ± 0.029

3.839 ± 0.03

3.832 ± 0.03

4.478 ± 0.035

5.888 ± 0.032

5.012 ± 0.026

6.878 ± 0.037

1.057 ± 0.018

3.437 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski