Melanoplus sanguinipes entomopoxvirus (MsEPV)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Entomopoxvirinae; Deltaentomopoxvirus

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 261 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9YW44|Q9YW44_MSEPV ORF MSV048 putative lipase similar to Rhizopus niveus lipase GB:D12680 OS=Melanoplus sanguinipes entomopoxvirus OX=83191 GN=MSV048 PE=4 SV=1
MM1 pKa = 7.03KK2 pKa = 9.48TISGLGVIAVFIFCIEE18 pKa = 4.21VYY20 pKa = 9.74TVVLVATYY28 pKa = 10.76NAIDD32 pKa = 4.2KK33 pKa = 10.81LFDD36 pKa = 5.04DD37 pKa = 5.79IIDD40 pKa = 3.71ISLSLSFIIYY50 pKa = 8.31LTFSLIGDD58 pKa = 3.63IGGSFFLL65 pKa = 5.31

Molecular weight: 7.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9YW24|Q9YW24_MSEPV Uncharacterized protein OS=Melanoplus sanguinipes entomopoxvirus OX=83191 GN=MSV068 PE=4 SV=1
MM1 pKa = 8.08WITISPRR8 pKa = 11.84PIWFSTSFQWQFYY21 pKa = 8.99TSTAFWTSIKK31 pKa = 10.3KK32 pKa = 9.64QFSAWSYY39 pKa = 9.34TNTAFWTSIIWPWPWIRR56 pKa = 11.84IRR58 pKa = 11.84QNCLMCIFIYY68 pKa = 10.33NWIFITII75 pKa = 4.01

Molecular weight: 9.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

261

0

261

71400

60

1319

273.6

32.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.835 ± 0.098

2.164 ± 0.135

5.711 ± 0.109

5.249 ± 0.153

5.339 ± 0.144

2.144 ± 0.092

1.696 ± 0.099

13.93 ± 0.184

9.985 ± 0.213

9.521 ± 0.194

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.818 ± 0.067

12.102 ± 0.19

2.21 ± 0.076

2.203 ± 0.097

1.859 ± 0.065

6.877 ± 0.16

4.401 ± 0.126

3.681 ± 0.138

0.49 ± 0.049

6.786 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski