Hubei sobemo-like virus 40

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEJ8|A0A1L3KEJ8_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 40 OX=1923228 PE=4 SV=1
MM1 pKa = 6.85TQAPTIGEE9 pKa = 4.02WLFKK13 pKa = 11.31GEE15 pKa = 4.83LEE17 pKa = 4.25PDD19 pKa = 3.53PTRR22 pKa = 11.84KK23 pKa = 9.41EE24 pKa = 4.22LLWLMVTDD32 pKa = 4.53VIEE35 pKa = 4.36GNYY38 pKa = 9.08EE39 pKa = 3.5HH40 pKa = 7.57LFRR43 pKa = 11.84VFVKK47 pKa = 10.57HH48 pKa = 6.04EE49 pKa = 3.7PHH51 pKa = 6.37TEE53 pKa = 3.62RR54 pKa = 11.84KK55 pKa = 9.68ALAKK59 pKa = 9.84RR60 pKa = 11.84WRR62 pKa = 11.84LIIASSLPVQIVWQMVFGNIEE83 pKa = 4.12DD84 pKa = 4.86ALLQQTGRR92 pKa = 11.84TPSAYY97 pKa = 10.1GLVYY101 pKa = 10.41GAGGWKK107 pKa = 10.04RR108 pKa = 11.84FRR110 pKa = 11.84DD111 pKa = 3.46MLVQRR116 pKa = 11.84KK117 pKa = 8.64VNLCVDD123 pKa = 3.56KK124 pKa = 11.38SGWDD128 pKa = 3.42MNSPGWVYY136 pKa = 9.38EE137 pKa = 3.92LCLEE141 pKa = 4.08LRR143 pKa = 11.84KK144 pKa = 9.84RR145 pKa = 11.84LCTDD149 pKa = 3.98LTPAAEE155 pKa = 4.07RR156 pKa = 11.84VMDD159 pKa = 3.75MLYY162 pKa = 10.47RR163 pKa = 11.84DD164 pKa = 4.77AYY166 pKa = 10.49QSSKK170 pKa = 11.13LIFGDD175 pKa = 4.02GTILEE180 pKa = 4.31QQFSGFMKK188 pKa = 10.24SGLFVTISDD197 pKa = 3.93NSFSQYY203 pKa = 10.13FLHH206 pKa = 7.01VLACLRR212 pKa = 11.84LRR214 pKa = 11.84IPLGTFYY221 pKa = 10.75ATGDD225 pKa = 3.67DD226 pKa = 4.7TIQSLPPNVEE236 pKa = 4.0EE237 pKa = 4.35YY238 pKa = 11.01LDD240 pKa = 3.67HH241 pKa = 7.71LEE243 pKa = 4.16YY244 pKa = 10.83AGCEE248 pKa = 3.86VKK250 pKa = 10.43EE251 pKa = 4.07YY252 pKa = 10.1MNGYY256 pKa = 9.37QFMGFEE262 pKa = 4.26LKK264 pKa = 10.44DD265 pKa = 3.77YY266 pKa = 10.17GPHH269 pKa = 6.96PIYY272 pKa = 10.39LGKK275 pKa = 10.13HH276 pKa = 5.02FWNLLHH282 pKa = 7.3QEE284 pKa = 4.37DD285 pKa = 4.79EE286 pKa = 4.37NLEE289 pKa = 4.06QTIDD293 pKa = 3.58SYY295 pKa = 12.0LLNYY299 pKa = 9.88CKK301 pKa = 10.31VDD303 pKa = 3.31EE304 pKa = 4.72AFDD307 pKa = 3.82YY308 pKa = 11.15LRR310 pKa = 11.84ALSIEE315 pKa = 4.29LGYY318 pKa = 11.0APRR321 pKa = 11.84SKK323 pKa = 9.9QWYY326 pKa = 8.5RR327 pKa = 11.84FLMDD331 pKa = 4.11NPLALEE337 pKa = 4.53GNWTRR342 pKa = 11.84PGFVDD347 pKa = 3.5PASRR351 pKa = 11.84LGG353 pKa = 3.41

Molecular weight: 40.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEJ8|A0A1L3KEJ8_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 40 OX=1923228 PE=4 SV=1
MM1 pKa = 6.62YY2 pKa = 9.26TNLYY6 pKa = 9.1PHH8 pKa = 6.85FSAAKK13 pKa = 9.5SLAFQSGIEE22 pKa = 4.0KK23 pKa = 10.43SALVGKK29 pKa = 10.26AVTEE33 pKa = 4.26TVKK36 pKa = 10.92ARR38 pKa = 11.84VNLTRR43 pKa = 11.84LAVEE47 pKa = 4.42TVKK50 pKa = 10.77EE51 pKa = 4.16DD52 pKa = 3.88PLRR55 pKa = 11.84AALTVTSIVSAGWVLKK71 pKa = 10.43KK72 pKa = 10.37AVPFAYY78 pKa = 9.43RR79 pKa = 11.84KK80 pKa = 8.14YY81 pKa = 9.89RR82 pKa = 11.84EE83 pKa = 3.92WRR85 pKa = 11.84EE86 pKa = 3.98RR87 pKa = 11.84PWPVIKK93 pKa = 10.0EE94 pKa = 4.14APSYY98 pKa = 10.11EE99 pKa = 4.24PEE101 pKa = 4.13TAVEE105 pKa = 4.94GSVEE109 pKa = 4.35TNMSFARR116 pKa = 11.84CQGALCVAEE125 pKa = 4.24GQTWKK130 pKa = 9.36TVGGCFRR137 pKa = 11.84LTVGSKK143 pKa = 10.87DD144 pKa = 3.64LLLTAGHH151 pKa = 6.37NLVEE155 pKa = 4.32GEE157 pKa = 4.28RR158 pKa = 11.84YY159 pKa = 9.43AVSRR163 pKa = 11.84MGKK166 pKa = 9.16IKK168 pKa = 10.43EE169 pKa = 4.38LGDD172 pKa = 3.06VWSAKK177 pKa = 10.44DD178 pKa = 3.19ADD180 pKa = 4.24QIYY183 pKa = 10.8SPVTDD188 pKa = 3.91IVAIPLPDD196 pKa = 3.39SFFSGLSMRR205 pKa = 11.84SATTIALPSSTNAQITGALGKK226 pKa = 10.76GSVGKK231 pKa = 8.94LHH233 pKa = 7.22PLGQFGRR240 pKa = 11.84VEE242 pKa = 4.2YY243 pKa = 10.79AGTTAGGYY251 pKa = 9.21SGCPYY256 pKa = 10.26IVGEE260 pKa = 4.24RR261 pKa = 11.84VAAIHH266 pKa = 5.97TNGGRR271 pKa = 11.84KK272 pKa = 9.27NEE274 pKa = 3.82GWEE277 pKa = 3.85IKK279 pKa = 10.5YY280 pKa = 9.76VEE282 pKa = 4.48CLLYY286 pKa = 11.07AHH288 pKa = 6.5FVVSNEE294 pKa = 4.08STVGSEE300 pKa = 3.83DD301 pKa = 3.18AARR304 pKa = 11.84RR305 pKa = 11.84AAQEE309 pKa = 3.87EE310 pKa = 4.59HH311 pKa = 7.48DD312 pKa = 3.99IAPYY316 pKa = 10.8DD317 pKa = 4.26DD318 pKa = 4.89DD319 pKa = 5.97SIIIRR324 pKa = 11.84SRR326 pKa = 11.84KK327 pKa = 6.34TGKK330 pKa = 10.24YY331 pKa = 7.82YY332 pKa = 9.6RR333 pKa = 11.84TRR335 pKa = 11.84EE336 pKa = 3.96TVYY339 pKa = 10.83EE340 pKa = 3.89EE341 pKa = 4.39LMRR344 pKa = 11.84AKK346 pKa = 10.07QSYY349 pKa = 10.38ANNPDD354 pKa = 2.7RR355 pKa = 11.84WADD358 pKa = 3.57QVEE361 pKa = 4.27VDD363 pKa = 5.41LLQQEE368 pKa = 4.68LDD370 pKa = 3.6SYY372 pKa = 10.83MPEE375 pKa = 4.32CASEE379 pKa = 4.28AGNAKK384 pKa = 9.0TPAQAGVGQLDD395 pKa = 3.39GDD397 pKa = 3.86MSVKK401 pKa = 10.3QFLSLLRR408 pKa = 11.84EE409 pKa = 3.87NRR411 pKa = 11.84ALSMSEE417 pKa = 4.07SSEE420 pKa = 3.75QSQLSRR426 pKa = 11.84RR427 pKa = 11.84QRR429 pKa = 11.84RR430 pKa = 11.84QAYY433 pKa = 8.31FGRR436 pKa = 11.84RR437 pKa = 11.84MGLEE441 pKa = 3.48RR442 pKa = 11.84QANIRR447 pKa = 11.84RR448 pKa = 11.84RR449 pKa = 11.84SPTPARR455 pKa = 11.84KK456 pKa = 9.25

Molecular weight: 50.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

809

353

456

404.5

45.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.158 ± 2.048

1.483 ± 0.145

4.821 ± 0.755

7.417 ± 0.224

3.585 ± 1.014

7.664 ± 0.2

1.731 ± 0.359

3.832 ± 0.09

4.944 ± 0.276

9.765 ± 1.997

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.472 ± 0.431

3.09 ± 0.207

4.45 ± 0.245

4.203 ± 0.031

6.922 ± 0.841

6.799 ± 1.328

5.315 ± 0.524

6.551 ± 0.783

2.101 ± 0.49

4.697 ± 0.459
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski