Escherichia phage CICC 80001

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Teseptimavirus; Escherichia virus CICC80001

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088F904|A0A088F904_9CAUD Protein kinase OS=Escherichia phage CICC 80001 OX=1527506 PE=4 SV=1
MM1 pKa = 7.48AMSNMTYY8 pKa = 11.18NNVFDD13 pKa = 4.72HH14 pKa = 6.93AYY16 pKa = 10.52EE17 pKa = 3.87MLKK20 pKa = 10.85EE21 pKa = 3.86NTRR24 pKa = 11.84YY25 pKa = 10.73DD26 pKa = 4.49DD27 pKa = 5.15IRR29 pKa = 11.84DD30 pKa = 3.58TDD32 pKa = 3.94DD33 pKa = 3.47LHH35 pKa = 7.49DD36 pKa = 4.63AVHH39 pKa = 6.27MAADD43 pKa = 3.73SAVPHH48 pKa = 6.4YY49 pKa = 10.26YY50 pKa = 10.6SDD52 pKa = 3.78VFSVMASEE60 pKa = 5.5GIDD63 pKa = 4.5LEE65 pKa = 4.76FEE67 pKa = 4.73DD68 pKa = 5.02SGLMPDD74 pKa = 3.4TKK76 pKa = 10.94DD77 pKa = 3.64VIRR80 pKa = 11.84ILQARR85 pKa = 11.84IYY87 pKa = 9.25EE88 pKa = 4.14QLTIDD93 pKa = 3.63LWEE96 pKa = 4.54DD97 pKa = 3.59TEE99 pKa = 5.28DD100 pKa = 4.25LLNEE104 pKa = 3.92YY105 pKa = 10.46LEE107 pKa = 4.27EE108 pKa = 4.14VEE110 pKa = 4.66EE111 pKa = 4.45YY112 pKa = 10.76EE113 pKa = 4.86EE114 pKa = 4.49EE115 pKa = 4.21EE116 pKa = 4.17

Molecular weight: 13.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088FBR7|A0A088FBR7_9CAUD DNA ligase OS=Escherichia phage CICC 80001 OX=1527506 PE=4 SV=1
MM1 pKa = 7.49LKK3 pKa = 10.15HH4 pKa = 5.88YY5 pKa = 10.29VMPIHH10 pKa = 6.52TKK12 pKa = 10.57NGATVCTPDD21 pKa = 3.38GFAMKK26 pKa = 10.26QRR28 pKa = 11.84IEE30 pKa = 3.89RR31 pKa = 11.84LKK33 pKa = 10.95RR34 pKa = 11.84EE35 pKa = 3.7LRR37 pKa = 11.84TNRR40 pKa = 11.84KK41 pKa = 9.14LNNII45 pKa = 4.0

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11568

37

1318

236.1

26.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.146 ± 0.462

1.115 ± 0.205

6.475 ± 0.272

7.46 ± 0.35

3.484 ± 0.209

7.296 ± 0.345

1.98 ± 0.203

5.29 ± 0.159

6.846 ± 0.37

7.746 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.121 ± 0.205

4.365 ± 0.252

3.432 ± 0.178

3.683 ± 0.342

5.628 ± 0.174

6.068 ± 0.274

5.403 ± 0.197

6.414 ± 0.246

1.642 ± 0.198

3.406 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski