Mycolicibacterium moriokaense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group;

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5948 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X0HIP7|A0A1X0HIP7_9MYCO Hydrolase OS=Mycolicibacterium moriokaense OX=39691 GN=BST36_04885 PE=4 SV=1
MM1 pKa = 7.47GALTGCSSGSAADD14 pKa = 4.27LAVGDD19 pKa = 4.3CLKK22 pKa = 10.31TGGTAEE28 pKa = 4.11RR29 pKa = 11.84PEE31 pKa = 4.18VTKK34 pKa = 10.55VDD36 pKa = 3.94CGSPEE41 pKa = 4.53SNFKK45 pKa = 10.34VAATVEE51 pKa = 4.06NSDD54 pKa = 4.2LCPADD59 pKa = 3.2VDD61 pKa = 4.11SYY63 pKa = 11.14YY64 pKa = 11.65SMRR67 pKa = 11.84GAFSDD72 pKa = 3.8ASTTICMDD80 pKa = 3.55IDD82 pKa = 4.05WVVGDD87 pKa = 5.14CMSIDD92 pKa = 3.63PTNDD96 pKa = 3.06KK97 pKa = 11.39DD98 pKa = 4.1PVRR101 pKa = 11.84ANCDD105 pKa = 3.49DD106 pKa = 3.85ASVPNRR112 pKa = 11.84QRR114 pKa = 11.84VTEE117 pKa = 4.02ILDD120 pKa = 3.57DD121 pKa = 3.85VANVDD126 pKa = 3.48QCATGVGYY134 pKa = 9.05PYY136 pKa = 10.42DD137 pKa = 3.63EE138 pKa = 5.28RR139 pKa = 11.84NFTVCVEE146 pKa = 4.36DD147 pKa = 3.82VAA149 pKa = 5.65

Molecular weight: 15.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X0HDA7|A0A1X0HDA7_9MYCO DUF2236 domain-containing protein OS=Mycolicibacterium moriokaense OX=39691 GN=BST36_11800 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSSRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.75GRR42 pKa = 11.84RR43 pKa = 11.84KK44 pKa = 8.65LTAA47 pKa = 3.95

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5948

0

5948

1886812

33

3423

317.2

34.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.492 ± 0.043

0.817 ± 0.009

6.379 ± 0.024

5.592 ± 0.029

3.167 ± 0.019

8.621 ± 0.028

2.155 ± 0.014

4.547 ± 0.021

2.417 ± 0.022

9.707 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.014

2.352 ± 0.015

5.771 ± 0.025

2.95 ± 0.016

7.025 ± 0.03

5.373 ± 0.018

6.02 ± 0.021

8.719 ± 0.027

1.536 ± 0.015

2.224 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski