Lactobacillus phage PLE3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pleetrevirus; Lactobacillus virus PLE3

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0YC09|A0A1B0YC09_9CAUD Uncharacterized protein OS=Lactobacillus phage PLE3 OX=1815510 GN=PLE3_50 PE=4 SV=1
MM1 pKa = 7.21EE2 pKa = 5.49RR3 pKa = 11.84EE4 pKa = 3.98AMIDD8 pKa = 3.53FLTRR12 pKa = 11.84IYY14 pKa = 10.78PEE16 pKa = 4.02VPAFAFEE23 pKa = 4.19QMPDD27 pKa = 3.4EE28 pKa = 4.08QLKK31 pKa = 9.67GHH33 pKa = 5.38VDD35 pKa = 2.8EE36 pKa = 5.35WLAEE40 pKa = 4.33DD41 pKa = 4.79ADD43 pKa = 3.98QLAMGG48 pKa = 4.89

Molecular weight: 5.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0Y690|A0A1B0Y690_9CAUD Holin OS=Lactobacillus phage PLE3 OX=1815510 GN=PLE3_22 PE=4 SV=1
MM1 pKa = 7.39SKK3 pKa = 11.07ASISYY8 pKa = 9.76NMQIKK13 pKa = 10.5GMDD16 pKa = 3.58KK17 pKa = 10.68LVAGLLRR24 pKa = 11.84RR25 pKa = 11.84AKK27 pKa = 9.75MDD29 pKa = 3.27VVKK32 pKa = 10.79QIVKK36 pKa = 9.63QQTAQLQTRR45 pKa = 11.84AQQMTGTVYY54 pKa = 10.9AHH56 pKa = 6.29PTGATKK62 pKa = 10.43RR63 pKa = 11.84GIKK66 pKa = 10.24LSLEE70 pKa = 4.25AGGLTGIVGMSMEE83 pKa = 4.27YY84 pKa = 10.63NPYY87 pKa = 8.98TEE89 pKa = 3.93NGTRR93 pKa = 11.84FMRR96 pKa = 11.84ARR98 pKa = 11.84PVLKK102 pKa = 10.0PAFLYY107 pKa = 10.71QKK109 pKa = 10.32VQFINQLKK117 pKa = 9.36QAAKK121 pKa = 10.35

Molecular weight: 13.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12475

42

1111

207.9

23.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.689 ± 0.599

0.417 ± 0.102

6.661 ± 0.392

5.475 ± 0.582

3.752 ± 0.206

6.469 ± 0.489

1.731 ± 0.203

6.02 ± 0.308

7.158 ± 0.479

7.8 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.774 ± 0.245

5.138 ± 0.256

3.776 ± 0.228

4.569 ± 0.252

4.104 ± 0.425

7.423 ± 0.44

6.894 ± 0.507

5.892 ± 0.285

1.403 ± 0.161

3.856 ± 0.346
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski