Microviridae Fen7918_21

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UMI2|A0A0G2UMI2_9VIRU Uncharacterized protein OS=Microviridae Fen7918_21 OX=1655661 PE=4 SV=1
MM1 pKa = 7.36SRR3 pKa = 11.84NIFNSVKK10 pKa = 9.81MMRR13 pKa = 11.84PKK15 pKa = 10.84KK16 pKa = 10.22NVFDD20 pKa = 3.83LTHH23 pKa = 7.13DD24 pKa = 3.88VKK26 pKa = 10.37QTTRR30 pKa = 11.84FGEE33 pKa = 4.12LAPCLWLEE41 pKa = 4.46CVPGDD46 pKa = 3.66KK47 pKa = 10.61FKK49 pKa = 10.89IGAEE53 pKa = 4.0TLIRR57 pKa = 11.84FAPLISPVMHH67 pKa = 6.86RR68 pKa = 11.84MNASIHH74 pKa = 5.52YY75 pKa = 8.07FFVPYY80 pKa = 10.33RR81 pKa = 11.84ILWPNWEE88 pKa = 4.52DD89 pKa = 4.09YY90 pKa = 9.2ITNTSSVHH98 pKa = 5.35APPYY102 pKa = 10.1VDD104 pKa = 3.55YY105 pKa = 11.22SQIATSHH112 pKa = 5.0QRR114 pKa = 11.84LGDD117 pKa = 3.6FLGIPPQVQSGNGHH131 pKa = 6.47PKK133 pKa = 9.68VSAFPFAAYY142 pKa = 10.26LKK144 pKa = 10.3IYY146 pKa = 10.07DD147 pKa = 4.76DD148 pKa = 4.24YY149 pKa = 12.01YY150 pKa = 11.32RR151 pKa = 11.84DD152 pKa = 3.5QNLIQPAWSPLTDD165 pKa = 3.65GLQVANLVDD174 pKa = 4.61LVTMRR179 pKa = 11.84NRR181 pKa = 11.84AWEE184 pKa = 3.98HH185 pKa = 6.98DD186 pKa = 4.46YY187 pKa = 9.61FTASLPWAQKK197 pKa = 9.17GAQVEE202 pKa = 4.42IPLGDD207 pKa = 3.24ITLGQPAGGPNQFLRR222 pKa = 11.84RR223 pKa = 11.84GIDD226 pKa = 3.23GSTEE230 pKa = 3.85GNIAALGTDD239 pKa = 3.45TPGFLVDD246 pKa = 4.23AAGQVPVNIDD256 pKa = 3.49PNGSLINEE264 pKa = 3.97ATTINDD270 pKa = 2.96LRR272 pKa = 11.84RR273 pKa = 11.84AFRR276 pKa = 11.84LQEE279 pKa = 3.89WLEE282 pKa = 3.97KK283 pKa = 10.14NARR286 pKa = 11.84AGTRR290 pKa = 11.84YY291 pKa = 9.67IEE293 pKa = 4.73NILAHH298 pKa = 6.4FGVNSSDD305 pKa = 3.63KK306 pKa = 10.04RR307 pKa = 11.84LQRR310 pKa = 11.84PEE312 pKa = 4.34YY313 pKa = 9.97ICGLKK318 pKa = 9.33TPVVISEE325 pKa = 4.11VLNTTGAPDD334 pKa = 3.88GLAQGNMAGHH344 pKa = 7.44GIAAHH349 pKa = 5.14SAKK352 pKa = 10.47DD353 pKa = 3.51GSYY356 pKa = 9.86SCEE359 pKa = 3.7EE360 pKa = 4.31HH361 pKa = 6.53GLIMGVMSIMPNTAYY376 pKa = 10.25QQGIHH381 pKa = 6.38KK382 pKa = 9.67SWSKK386 pKa = 10.76FDD388 pKa = 3.4QLDD391 pKa = 4.0YY392 pKa = 10.84FWPTFAHH399 pKa = 6.84LGEE402 pKa = 4.2QEE404 pKa = 4.19VLNQEE409 pKa = 4.6VYY411 pKa = 10.62AQSSDD416 pKa = 3.26PGGTFGYY423 pKa = 9.46VPRR426 pKa = 11.84YY427 pKa = 9.67SEE429 pKa = 4.3YY430 pKa = 10.57KK431 pKa = 10.27FMPNRR436 pKa = 11.84VATSFRR442 pKa = 11.84DD443 pKa = 3.8TLAFWHH449 pKa = 6.52MGRR452 pKa = 11.84IFATEE457 pKa = 3.55PALNQDD463 pKa = 3.82FVEE466 pKa = 4.57CTTDD470 pKa = 3.0NRR472 pKa = 11.84IFAVTDD478 pKa = 3.73PDD480 pKa = 3.55VDD482 pKa = 4.7NIYY485 pKa = 10.94CDD487 pKa = 2.99ILNRR491 pKa = 11.84ITAIRR496 pKa = 11.84PMPKK500 pKa = 9.88YY501 pKa = 7.48GTPMII506 pKa = 4.76

Molecular weight: 56.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UMI2|A0A0G2UMI2_9VIRU Uncharacterized protein OS=Microviridae Fen7918_21 OX=1655661 PE=4 SV=1
MM1 pKa = 6.9TQINTYY7 pKa = 9.47AQNATIWDD15 pKa = 4.26LVAILASDD23 pKa = 4.89LLTIAIIYY31 pKa = 7.6FIKK34 pKa = 10.14KK35 pKa = 8.68HH36 pKa = 5.94KK37 pKa = 10.41KK38 pKa = 9.55HH39 pKa = 6.04GLQKK43 pKa = 9.64NTWQAFLRR51 pKa = 11.84SRR53 pKa = 11.84PP54 pKa = 3.57

Molecular weight: 6.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1293

54

506

258.6

29.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.425 ± 0.961

0.928 ± 0.349

5.182 ± 0.612

4.099 ± 0.568

3.635 ± 0.644

6.497 ± 0.792

2.552 ± 0.721

6.11 ± 1.091

6.032 ± 1.605

8.507 ± 0.585

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.475 ± 0.279

6.961 ± 0.962

5.182 ± 0.892

6.342 ± 1.549

4.95 ± 0.704

6.11 ± 0.46

7.115 ± 0.515

4.408 ± 0.831

1.779 ± 0.211

3.712 ± 0.456
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski