Streptomyces phage Annadreamy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Annadreamyvirus; Streptomyces virus Annadreamy

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 227 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345GTJ2|A0A345GTJ2_9CAUD Uncharacterized protein OS=Streptomyces phage Annadreamy OX=2250335 GN=179 PE=4 SV=1
MM1 pKa = 7.57EE2 pKa = 5.96EE3 pKa = 3.61IVSYY7 pKa = 11.1SFAVDD12 pKa = 3.41IYY14 pKa = 8.63KK15 pKa = 9.53TEE17 pKa = 4.16SGTYY21 pKa = 9.49AYY23 pKa = 10.34GVFQEE28 pKa = 4.99LEE30 pKa = 4.27SEE32 pKa = 4.46TEE34 pKa = 4.55DD35 pKa = 4.86DD36 pKa = 4.38LQLLEE41 pKa = 4.36TGEE44 pKa = 4.62ADD46 pKa = 3.92TLAEE50 pKa = 4.22AADD53 pKa = 3.95MASKK57 pKa = 10.56SIRR60 pKa = 11.84TLFSVV65 pKa = 3.97

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345GTS7|A0A345GTS7_9CAUD Uncharacterized protein OS=Streptomyces phage Annadreamy OX=2250335 GN=222 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.77LMTFEE7 pKa = 5.25FSTEE11 pKa = 3.62YY12 pKa = 9.09KK13 pKa = 10.31TYY15 pKa = 10.45IKK17 pKa = 10.39SPKK20 pKa = 8.22WKK22 pKa = 9.72RR23 pKa = 11.84ICSRR27 pKa = 11.84YY28 pKa = 6.76WAVYY32 pKa = 9.12GRR34 pKa = 11.84KK35 pKa = 8.94CQACGKK41 pKa = 9.55RR42 pKa = 11.84RR43 pKa = 11.84EE44 pKa = 4.34LHH46 pKa = 5.32VHH48 pKa = 5.0HH49 pKa = 6.58HH50 pKa = 5.93TYY52 pKa = 11.23ARR54 pKa = 11.84FGRR57 pKa = 11.84EE58 pKa = 3.99LLTDD62 pKa = 4.07LTGLCHH68 pKa = 6.53DD69 pKa = 4.3CHH71 pKa = 7.13RR72 pKa = 11.84RR73 pKa = 11.84VHH75 pKa = 5.18QRR77 pKa = 11.84HH78 pKa = 4.99RR79 pKa = 11.84ANRR82 pKa = 11.84RR83 pKa = 11.84VSLEE87 pKa = 4.26LVTKK91 pKa = 10.29QYY93 pKa = 9.57VQAMKK98 pKa = 10.67NKK100 pKa = 9.96KK101 pKa = 9.45LL102 pKa = 3.6

Molecular weight: 12.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

227

0

227

35709

29

2076

157.3

17.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.808 ± 0.321

1.07 ± 0.117

6.581 ± 0.178

7.175 ± 0.351

4.013 ± 0.138

7.466 ± 0.232

1.952 ± 0.136

5.268 ± 0.136

6.192 ± 0.251

7.147 ± 0.168

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.89 ± 0.127

4.579 ± 0.149

3.753 ± 0.154

3.134 ± 0.161

5.64 ± 0.223

5.976 ± 0.302

6.116 ± 0.358

7.135 ± 0.219

1.977 ± 0.111

4.131 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski