Bacillus pumilus (strain SAFR-032)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus pumilus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3700 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A8FBX4|FABH_BACP2 3-oxoacyl-[acyl-carrier-protein] synthase 3 OS=Bacillus pumilus (strain SAFR-032) OX=315750 GN=fabH PE=3 SV=1
MM1 pKa = 7.37PAIVGPIYY9 pKa = 10.47INSISGGVVNVGDD22 pKa = 4.43SFYY25 pKa = 10.99LSPKK29 pKa = 9.76SSSKK33 pKa = 10.67SALGSGAGNTGDD45 pKa = 5.55FLILNNGISATNYY58 pKa = 10.02VDD60 pKa = 5.51PDD62 pKa = 3.91LNDD65 pKa = 3.16QNMVGNAA72 pKa = 3.32

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U2ITL7|A0A0U2ITL7_BACP2 Uncharacterized protein OS=Bacillus pumilus (strain SAFR-032) OX=315750 GN=BPUM_04110 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.27RR12 pKa = 11.84SKK14 pKa = 9.34VHH16 pKa = 5.82GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.54NGRR28 pKa = 11.84LVLKK32 pKa = 10.3RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.79GRR39 pKa = 11.84KK40 pKa = 8.74KK41 pKa = 10.66LSAA44 pKa = 3.95

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3699

1

3700

1071977

24

3568

289.7

32.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.382 ± 0.044

0.719 ± 0.011

5.183 ± 0.032

7.31 ± 0.06

4.52 ± 0.035

6.791 ± 0.062

2.499 ± 0.029

7.34 ± 0.041

7.032 ± 0.049

9.796 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.917 ± 0.023

3.662 ± 0.029

3.601 ± 0.022

4.336 ± 0.039

3.992 ± 0.03

6.07 ± 0.036

5.597 ± 0.047

6.928 ± 0.033

0.948 ± 0.014

3.378 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski