Microbacterium sediminis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2529 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B9NB39|A0A1B9NB39_9MICO Histidinol-phosphate aminotransferase OS=Microbacterium sediminis OX=904291 GN=hisC PE=3 SV=1
MM1 pKa = 7.18NVRR4 pKa = 11.84RR5 pKa = 11.84SWLAAAAVTLSAGLALTGCAGAGNTGNTNGGSGDD39 pKa = 3.78EE40 pKa = 4.09NTIVWWHH47 pKa = 5.99NSNTGEE53 pKa = 3.97GKK55 pKa = 10.27EE56 pKa = 4.34YY57 pKa = 10.21YY58 pKa = 10.19DD59 pKa = 3.62QVAADD64 pKa = 4.67FEE66 pKa = 4.52AAHH69 pKa = 6.54EE70 pKa = 4.41GVTVQVEE77 pKa = 4.35AMQHH81 pKa = 5.21EE82 pKa = 4.71DD83 pKa = 3.72MLTKK87 pKa = 10.54LQAAFQGGDD96 pKa = 3.0AAQIPDD102 pKa = 3.54VYY104 pKa = 9.67MSRR107 pKa = 11.84GGGEE111 pKa = 4.03LKK113 pKa = 10.68SEE115 pKa = 4.3VEE117 pKa = 4.15GGVVRR122 pKa = 11.84DD123 pKa = 3.94LTEE126 pKa = 4.37DD127 pKa = 3.45AADD130 pKa = 4.1TISTIEE136 pKa = 4.38SFTEE140 pKa = 4.12QYY142 pKa = 10.34TVDD145 pKa = 3.8DD146 pKa = 4.01KK147 pKa = 11.97VYY149 pKa = 10.42ALPYY153 pKa = 10.03SIGIVGFWYY162 pKa = 10.75NKK164 pKa = 10.39DD165 pKa = 3.07LFAAAGIDD173 pKa = 3.51EE174 pKa = 4.59VSPNPTWEE182 pKa = 4.02EE183 pKa = 3.5FTGYY187 pKa = 10.11VDD189 pKa = 3.45KK190 pKa = 11.47LKK192 pKa = 10.91AAGTAPIAVGAGDD205 pKa = 3.89KK206 pKa = 10.12WPAAHH211 pKa = 5.66YY212 pKa = 8.9WYY214 pKa = 10.53YY215 pKa = 11.12NVVRR219 pKa = 11.84EE220 pKa = 4.14CEE222 pKa = 4.05YY223 pKa = 11.38DD224 pKa = 3.7VVEE227 pKa = 4.32AAIEE231 pKa = 4.22SGDD234 pKa = 3.73YY235 pKa = 10.77SDD237 pKa = 5.32EE238 pKa = 4.45CFLTAGEE245 pKa = 4.37HH246 pKa = 6.37LEE248 pKa = 4.09EE249 pKa = 4.31TLALEE254 pKa = 4.27PFNAGFLSTPAQSGPTSASGLLATGKK280 pKa = 10.61VGMEE284 pKa = 4.18LAGHH288 pKa = 6.36WEE290 pKa = 3.98PGVAGGLTEE299 pKa = 4.3NGEE302 pKa = 4.4VPPFLGWFAYY312 pKa = 7.59PTFDD316 pKa = 3.93GQGGAPDD323 pKa = 3.93DD324 pKa = 3.85QMGGGDD330 pKa = 3.61AWSVSTGAPDD340 pKa = 3.15IAVEE344 pKa = 4.02FAEE347 pKa = 4.37YY348 pKa = 10.69LLSDD352 pKa = 3.99EE353 pKa = 4.41VQIGFAEE360 pKa = 4.59LDD362 pKa = 3.24MGLPTNPAATDD373 pKa = 3.44SLANEE378 pKa = 4.35TLAQLIPVRR387 pKa = 11.84DD388 pKa = 4.04GGGKK392 pKa = 6.82TQLYY396 pKa = 10.19LDD398 pKa = 3.84TRR400 pKa = 11.84LGQSVGNALNDD411 pKa = 4.58AIALMFAGQAGPEE424 pKa = 4.48DD425 pKa = 3.47IVEE428 pKa = 4.34ALEE431 pKa = 4.24SASAMGG437 pKa = 3.92

Molecular weight: 45.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B9NA99|A0A1B9NA99_9MICO UPF0225 protein A7J15_07480 OS=Microbacterium sediminis OX=904291 GN=A7J15_07480 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.52GRR40 pKa = 11.84TEE42 pKa = 4.14LSAA45 pKa = 4.86

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2529

0

2529

822206

38

2094

325.1

34.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.159 ± 0.09

0.477 ± 0.012

6.149 ± 0.043

6.105 ± 0.047

3.032 ± 0.033

9.082 ± 0.037

1.925 ± 0.023

4.696 ± 0.037

1.833 ± 0.038

10.064 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.83 ± 0.023

1.79 ± 0.025

5.588 ± 0.036

2.73 ± 0.028

7.579 ± 0.064

4.901 ± 0.031

5.762 ± 0.047

8.858 ± 0.042

1.492 ± 0.022

1.949 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski