Daphne virus S

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5GR21|Q5GR21_9VIRU Movement protein TGB2 OS=Daphne virus S OX=216614 PE=3 SV=1
MM1 pKa = 7.54SLNISLSLLHH11 pKa = 6.44VGLIVFTVLCILGSLYY27 pKa = 10.68LSPSGSQCVIIITGEE42 pKa = 3.94SIKK45 pKa = 10.52ILNCEE50 pKa = 3.84MTPAFLEE57 pKa = 4.44YY58 pKa = 10.83AKK60 pKa = 10.23GLHH63 pKa = 5.55VEE65 pKa = 4.9RR66 pKa = 11.84II67 pKa = 3.8

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5GR22|Q5GR22_9VIRU Triple gene block 1 OS=Daphne virus S OX=216614 PE=4 SV=1
MM1 pKa = 7.95PLTPPPDD8 pKa = 3.32YY9 pKa = 8.4TTAVLVAAATLGATLFISSLTRR31 pKa = 11.84NTQPQVGDD39 pKa = 4.19NIHH42 pKa = 6.25SLPHH46 pKa = 5.15GGYY49 pKa = 9.88YY50 pKa = 10.01KK51 pKa = 10.91DD52 pKa = 3.39GTKK55 pKa = 9.98VVHH58 pKa = 6.2YY59 pKa = 7.73GAPGKK64 pKa = 10.35FNSVEE69 pKa = 4.08FSRR72 pKa = 11.84DD73 pKa = 3.2CYY75 pKa = 8.85FQPWFVIVLLTFLIILSSKK94 pKa = 10.26YY95 pKa = 9.9RR96 pKa = 11.84GHH98 pKa = 6.21TCAACRR104 pKa = 3.74

Molecular weight: 11.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2832

67

2004

472.0

53.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.662 ± 0.892

2.507 ± 0.631

4.908 ± 0.62

7.027 ± 1.067

5.191 ± 0.624

6.356 ± 0.281

2.754 ± 0.274

5.544 ± 0.721

5.897 ± 1.305

9.428 ± 0.739

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.295 ± 0.511

3.919 ± 0.57

4.308 ± 1.022

3.001 ± 0.242

6.109 ± 0.665

7.203 ± 0.342

4.732 ± 0.582

6.356 ± 0.624

1.095 ± 0.276

3.708 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski