Frigoribacterium sp. JB110

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Frigoribacterium; unclassified Frigoribacterium

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3430 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R4GCR7|A0A1R4GCR7_9MICO Uncharacterized protein OS=Frigoribacterium sp. JB110 OX=1434829 GN=CZ774_14100 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84AGRR5 pKa = 11.84PKK7 pKa = 10.49RR8 pKa = 11.84RR9 pKa = 11.84IAAAAAVLAAGAMLLSGCLSAFIPEE34 pKa = 4.54RR35 pKa = 11.84PADD38 pKa = 4.03PAPVTDD44 pKa = 4.86GVPSEE49 pKa = 5.2LIEE52 pKa = 6.04FYY54 pKa = 10.64DD55 pKa = 5.87QPPQWQDD62 pKa = 3.17CGPDD66 pKa = 3.72LQCATITAPLDD77 pKa = 3.1WHH79 pKa = 7.07DD80 pKa = 3.87PARR83 pKa = 11.84GEE85 pKa = 4.0IDD87 pKa = 3.53LALTRR92 pKa = 11.84YY93 pKa = 9.3LAEE96 pKa = 3.97QGAPDD101 pKa = 4.78GSLLTNPGGPGASGVSFVQSSAQYY125 pKa = 11.11LFADD129 pKa = 4.65DD130 pKa = 3.96VRR132 pKa = 11.84AAYY135 pKa = 9.98DD136 pKa = 3.15IVGFDD141 pKa = 3.44PRR143 pKa = 11.84GVGEE147 pKa = 4.29STAVEE152 pKa = 4.33CLDD155 pKa = 3.9DD156 pKa = 4.29AAMDD160 pKa = 3.74SYY162 pKa = 11.51LYY164 pKa = 10.15DD165 pKa = 3.71LPEE168 pKa = 4.34GEE170 pKa = 4.52RR171 pKa = 11.84GSAEE175 pKa = 3.79WEE177 pKa = 4.01RR178 pKa = 11.84EE179 pKa = 4.18LEE181 pKa = 4.05KK182 pKa = 11.38SNADD186 pKa = 3.49FAAGCEE192 pKa = 4.27EE193 pKa = 3.99NTGDD197 pKa = 4.14LLEE200 pKa = 5.7FITTEE205 pKa = 3.7QAARR209 pKa = 11.84DD210 pKa = 3.72LDD212 pKa = 4.48LIRR215 pKa = 11.84AVLGEE220 pKa = 4.0NEE222 pKa = 3.75LRR224 pKa = 11.84YY225 pKa = 10.34LGFSYY230 pKa = 9.14GTFLGATYY238 pKa = 11.27ANLFPEE244 pKa = 4.63RR245 pKa = 11.84AGHH248 pKa = 6.48LVLDD252 pKa = 4.28AAIDD256 pKa = 3.93PSVPSAMVGAVQGVGFEE273 pKa = 4.31DD274 pKa = 3.82ALRR277 pKa = 11.84AYY279 pKa = 10.5LADD282 pKa = 4.69CGDD285 pKa = 4.29GGQCPFPGNVDD296 pKa = 4.03DD297 pKa = 6.26GMADD301 pKa = 3.76LGALLAEE308 pKa = 4.54VDD310 pKa = 3.92DD311 pKa = 4.97RR312 pKa = 11.84PLVGADD318 pKa = 3.21GRR320 pKa = 11.84EE321 pKa = 4.01LGADD325 pKa = 3.55TLVTAIISSLYY336 pKa = 10.79SPLSWPDD343 pKa = 2.87LTAALEE349 pKa = 4.48GAQQGDD355 pKa = 3.26ASGAFALADD364 pKa = 3.49MYY366 pKa = 11.42NDD368 pKa = 3.84RR369 pKa = 11.84VNGTYY374 pKa = 9.97VANTTEE380 pKa = 4.62AFSAYY385 pKa = 10.49NCMDD389 pKa = 3.78YY390 pKa = 10.73PVSTAAEE397 pKa = 3.73TDD399 pKa = 3.38AADD402 pKa = 3.81EE403 pKa = 4.37LMSEE407 pKa = 4.4NAPTIAPYY415 pKa = 9.98WSGPDD420 pKa = 3.83LCEE423 pKa = 3.94QWPHH427 pKa = 6.1EE428 pKa = 4.17PTGTRR433 pKa = 11.84DD434 pKa = 3.2RR435 pKa = 11.84ITAEE439 pKa = 3.73GAAPILVVGTTGDD452 pKa = 3.61PATPYY457 pKa = 10.11QWAEE461 pKa = 3.74ALAEE465 pKa = 4.04QLDD468 pKa = 4.38SGTLITFEE476 pKa = 4.6GEE478 pKa = 3.94GHH480 pKa = 6.22GGYY483 pKa = 10.78GNSACINDD491 pKa = 3.72AVGDD495 pKa = 3.83FLIDD499 pKa = 3.26GTVPDD504 pKa = 5.58DD505 pKa = 4.64GLRR508 pKa = 11.84CQQ510 pKa = 4.2

Molecular weight: 53.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R4G7I6|A0A1R4G7I6_9MICO Secreted protein OS=Frigoribacterium sp. JB110 OX=1434829 GN=CZ774_12470 PE=4 SV=1
MM1 pKa = 7.29GRR3 pKa = 11.84GAVARR8 pKa = 11.84LGASRR13 pKa = 11.84HH14 pKa = 4.78RR15 pKa = 11.84RR16 pKa = 11.84LIRR19 pKa = 11.84RR20 pKa = 11.84SPARR24 pKa = 11.84KK25 pKa = 9.22ARR27 pKa = 11.84VSGSSLLKK35 pKa = 9.83VTLSLL40 pKa = 4.4

Molecular weight: 4.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3430

0

3430

1120900

37

3078

326.8

35.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.248 ± 0.062

0.514 ± 0.011

6.635 ± 0.04

6.061 ± 0.04

3.064 ± 0.025

9.066 ± 0.042

2.068 ± 0.02

4.653 ± 0.027

1.823 ± 0.027

9.73 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.982 ± 0.018

1.949 ± 0.017

5.154 ± 0.032

2.835 ± 0.023

7.354 ± 0.05

5.716 ± 0.027

6.009 ± 0.034

8.783 ± 0.036

1.455 ± 0.017

1.9 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski