Bivalve hepelivirus G

Taxonomy: Viruses; Riboviria; unclassified Riboviria

Average proteome isoelectric point is 7.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L4FQZ1|A0A1L4FQZ1_9VIRU Replicative protein OS=Bivalve hepelivirus G OX=1926998 PE=4 SV=1
MM1 pKa = 7.78DD2 pKa = 3.7VSHH5 pKa = 6.65TKK7 pKa = 10.88SMLEE11 pKa = 4.13LEE13 pKa = 4.67LEE15 pKa = 4.36LFGMLGVNQKK25 pKa = 10.5IIDD28 pKa = 4.71FYY30 pKa = 11.37ATMRR34 pKa = 11.84TKK36 pKa = 9.37WCNLYY41 pKa = 9.97QCRR44 pKa = 11.84EE45 pKa = 4.47GIAMLHH51 pKa = 5.59AWYY54 pKa = 8.2MQHH57 pKa = 6.73SGQPLTLTGNTLLNMAVIGFAYY79 pKa = 10.12RR80 pKa = 11.84IVGMLYY86 pKa = 10.56GAFKK90 pKa = 11.04GDD92 pKa = 3.94DD93 pKa = 2.99SHH95 pKa = 6.84MRR97 pKa = 11.84AKK99 pKa = 10.46KK100 pKa = 9.83ISTVPGRR107 pKa = 11.84TTQAYY112 pKa = 10.07ADD114 pKa = 3.71YY115 pKa = 10.1GYY117 pKa = 10.99KK118 pKa = 10.53LKK120 pKa = 10.65ISFEE124 pKa = 4.23KK125 pKa = 10.72VSEE128 pKa = 4.92FIANFVTPYY137 pKa = 11.04GFFPDD142 pKa = 3.49VVRR145 pKa = 11.84RR146 pKa = 11.84SVKK149 pKa = 10.09AVSKK153 pKa = 10.19IYY155 pKa = 10.19EE156 pKa = 4.44DD157 pKa = 3.69EE158 pKa = 4.4EE159 pKa = 4.16SWEE162 pKa = 4.02EE163 pKa = 3.78SRR165 pKa = 11.84VNLKK169 pKa = 10.16EE170 pKa = 3.76VLSVVNSADD179 pKa = 3.32KK180 pKa = 11.16YY181 pKa = 11.19KK182 pKa = 10.66IGIDD186 pKa = 3.56CAYY189 pKa = 10.7LHH191 pKa = 6.27YY192 pKa = 9.77RR193 pKa = 11.84DD194 pKa = 4.39KK195 pKa = 11.47GVAITRR201 pKa = 11.84EE202 pKa = 4.23QVSVLYY208 pKa = 10.31QYY210 pKa = 10.9LIQLSNTKK218 pKa = 10.3YY219 pKa = 10.92ADD221 pKa = 3.31AEE223 pKa = 4.61FIQSEE228 pKa = 4.2DD229 pKa = 3.22RR230 pKa = 11.84LTYY233 pKa = 10.34SDD235 pKa = 4.66NYY237 pKa = 9.86QSRR240 pKa = 3.68

Molecular weight: 27.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L4FQZ2|A0A1L4FQZ2_9VIRU Structural protein OS=Bivalve hepelivirus G OX=1926998 PE=4 SV=1
MM1 pKa = 7.11NAAAPLVDD9 pKa = 3.34NSMNFDD15 pKa = 3.75PMSDD19 pKa = 3.39STSMPEE25 pKa = 3.78QTHH28 pKa = 6.23GKK30 pKa = 7.53TLTPSQAFVCKK41 pKa = 8.95VTHH44 pKa = 6.89PPTTVPEE51 pKa = 4.26FAGLPTQDD59 pKa = 2.57TRR61 pKa = 11.84TQVVYY66 pKa = 11.08NMRR69 pKa = 11.84NIDD72 pKa = 3.99VIKK75 pKa = 10.11TPITFDD81 pKa = 3.23KK82 pKa = 10.99TSGKK86 pKa = 10.24YY87 pKa = 7.82EE88 pKa = 3.94QNSWTDD94 pKa = 3.08HH95 pKa = 5.97EE96 pKa = 5.25DD97 pKa = 3.25YY98 pKa = 11.47SFIVPTGARR107 pKa = 11.84IKK109 pKa = 10.35WFGCCYY115 pKa = 9.78QVNDD119 pKa = 3.98SQTTPTTADD128 pKa = 4.11AYY130 pKa = 10.8DD131 pKa = 3.4QDD133 pKa = 4.14LANVGVQDD141 pKa = 4.12NFDD144 pKa = 3.87FKK146 pKa = 11.16SWSSTVNLYY155 pKa = 10.19RR156 pKa = 11.84PCYY159 pKa = 10.14KK160 pKa = 10.65SITLYY165 pKa = 10.75PNVTAFNNQGIISAQQFNPNILFSGSTSALSYY197 pKa = 10.66DD198 pKa = 3.52KK199 pKa = 10.94PKK201 pKa = 11.27LFMQALDD208 pKa = 3.86HH209 pKa = 6.95LYY211 pKa = 10.86SQINDD216 pKa = 3.34KK217 pKa = 10.82LFCNEE222 pKa = 3.56EE223 pKa = 4.1THH225 pKa = 7.27VDD227 pKa = 3.77FHH229 pKa = 5.31QTVVEE234 pKa = 4.35SWFKK238 pKa = 9.52TRR240 pKa = 11.84KK241 pKa = 9.29LRR243 pKa = 11.84VRR245 pKa = 11.84GLNLDD250 pKa = 3.89PNNFLQILNIGNVGYY265 pKa = 10.89GSDD268 pKa = 3.55TSSLVPTPSQIAQNSMRR285 pKa = 11.84SYY287 pKa = 10.5QDD289 pKa = 3.01KK290 pKa = 10.36FINGAFVVSRR300 pKa = 11.84INTLSPKK307 pKa = 9.28WMAGSNTNIPVNGLYY322 pKa = 10.15EE323 pKa = 4.07CWLYY327 pKa = 11.28SIGPDD332 pKa = 3.49GRR334 pKa = 11.84PHH336 pKa = 7.08LVSLKK341 pKa = 10.78DD342 pKa = 3.37PVAAGTAAADD352 pKa = 4.02AKK354 pKa = 11.05PMLDD358 pKa = 3.91TLWSSDD364 pKa = 3.37MTWQFIRR371 pKa = 11.84VQGISPNVTASTAPAVVSPIAIKK394 pKa = 10.11HH395 pKa = 4.93YY396 pKa = 10.55YY397 pKa = 9.52GIEE400 pKa = 3.99AQPVWNGPWNGIARR414 pKa = 11.84MSPKK418 pKa = 9.98PSLSEE423 pKa = 3.72MQALMDD429 pKa = 4.31TFYY432 pKa = 11.07EE433 pKa = 4.51MPDD436 pKa = 3.41AMPAKK441 pKa = 8.78YY442 pKa = 10.37NAMGAFLPFLASALPHH458 pKa = 6.5ALRR461 pKa = 11.84FVKK464 pKa = 10.51DD465 pKa = 4.46LITSKK470 pKa = 10.54KK471 pKa = 9.63SKK473 pKa = 10.61NDD475 pKa = 3.13GTVGVATTTKK485 pKa = 10.12RR486 pKa = 11.84SKK488 pKa = 11.13ASTKK492 pKa = 10.2PISSNNDD499 pKa = 2.68KK500 pKa = 11.08QIIANLRR507 pKa = 11.84KK508 pKa = 9.9QIASMQLNNRR518 pKa = 11.84PPSKK522 pKa = 9.92RR523 pKa = 11.84RR524 pKa = 11.84KK525 pKa = 6.46TRR527 pKa = 11.84KK528 pKa = 8.83PRR530 pKa = 11.84SKK532 pKa = 10.39SSPIIINEE540 pKa = 3.88RR541 pKa = 11.84TKK543 pKa = 11.25

Molecular weight: 60.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2236

240

1453

745.3

84.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.903 ± 0.677

1.699 ± 0.295

6.127 ± 0.276

4.338 ± 0.824

4.249 ± 0.022

4.383 ± 0.383

2.326 ± 0.295

7.469 ± 0.921

7.737 ± 0.725

7.2 ± 0.382

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.728 ± 0.335

7.111 ± 0.784

3.98 ± 1.29

3.488 ± 0.601

4.07 ± 0.206

7.335 ± 0.848

6.664 ± 0.555

6.798 ± 0.346

0.984 ± 0.374

5.411 ± 0.822
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski