Escherichia phage vB_EcoS_MM01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Rogunavirinae; Wilsonroadvirus; unclassified Wilsonroadvirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482N547|A0A482N547_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_MM01 OX=2508188 GN=MM01_00007 PE=4 SV=1
MM1 pKa = 7.23ATIFTGKK8 pKa = 9.57IYY10 pKa = 10.33EE11 pKa = 4.21SRR13 pKa = 11.84KK14 pKa = 7.89TGYY17 pKa = 10.07EE18 pKa = 3.86YY19 pKa = 11.0EE20 pKa = 4.12LVITEE25 pKa = 4.17EE26 pKa = 5.34GYY28 pKa = 11.37VLDD31 pKa = 5.88DD32 pKa = 3.8GDD34 pKa = 4.32GYY36 pKa = 11.5AFEE39 pKa = 4.91VSPSYY44 pKa = 11.07KK45 pKa = 10.27DD46 pKa = 3.85DD47 pKa = 4.35LEE49 pKa = 4.53TVKK52 pKa = 10.87NAFGEE57 pKa = 4.31KK58 pKa = 9.32FDD60 pKa = 4.45EE61 pKa = 4.48VSEE64 pKa = 4.18

Molecular weight: 7.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482N2Y8|A0A482N2Y8_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_MM01 OX=2508188 GN=MM01_00032 PE=4 SV=1
MM1 pKa = 7.06TRR3 pKa = 11.84IAEE6 pKa = 4.36STGEE10 pKa = 3.86VDD12 pKa = 5.35KK13 pKa = 10.95RR14 pKa = 11.84TLNGNNGTQRR24 pKa = 11.84GEE26 pKa = 4.15DD27 pKa = 3.04KK28 pKa = 10.86KK29 pKa = 11.07EE30 pKa = 3.42RR31 pKa = 11.84SRR33 pKa = 11.84PYY35 pKa = 10.66GYY37 pKa = 8.65YY38 pKa = 8.79TLKK41 pKa = 11.15DD42 pKa = 3.57EE43 pKa = 4.59VRR45 pKa = 11.84ASLRR49 pKa = 11.84ARR51 pKa = 11.84LEE53 pKa = 3.8IVLNFYY59 pKa = 10.26GGKK62 pKa = 9.91SKK64 pKa = 10.42LAKK67 pKa = 9.73AANVTHH73 pKa = 6.58AVVSEE78 pKa = 3.82WCKK81 pKa = 10.78RR82 pKa = 11.84GMISAKK88 pKa = 10.02GAQKK92 pKa = 10.77LHH94 pKa = 5.55GKK96 pKa = 8.58YY97 pKa = 9.88KK98 pKa = 9.83RR99 pKa = 11.84TGEE102 pKa = 4.29GYY104 pKa = 8.5RR105 pKa = 11.84ASFIRR110 pKa = 11.84PDD112 pKa = 3.95LRR114 pKa = 11.84FDD116 pKa = 4.44SNGKK120 pKa = 8.82PLTLRR125 pKa = 11.84CDD127 pKa = 3.34RR128 pKa = 11.84RR129 pKa = 11.84EE130 pKa = 3.74MLRR133 pKa = 11.84VVKK136 pKa = 10.79KK137 pKa = 10.53EE138 pKa = 4.01DD139 pKa = 3.56LAKK142 pKa = 10.56KK143 pKa = 9.84PPARR147 pKa = 11.84SWRR150 pKa = 11.84KK151 pKa = 9.19IKK153 pKa = 10.59EE154 pKa = 3.96EE155 pKa = 3.89NKK157 pKa = 9.99KK158 pKa = 10.45KK159 pKa = 10.58RR160 pKa = 11.84EE161 pKa = 4.02SEE163 pKa = 4.16QNSTII168 pKa = 3.59

Molecular weight: 19.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13204

26

1118

194.2

21.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.717 ± 0.691

1.197 ± 0.213

5.93 ± 0.257

6.915 ± 0.299

3.915 ± 0.177

7.831 ± 0.491

1.325 ± 0.191

6.437 ± 0.212

6.824 ± 0.427

6.778 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.522 ± 0.279

4.968 ± 0.286

3.257 ± 0.269

3.893 ± 0.375

4.832 ± 0.236

6.892 ± 0.292

5.521 ± 0.265

7.21 ± 0.304

1.174 ± 0.12

3.862 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski