Prevotella sp. CAG:924

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2225 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5FE54|R5FE54_9BACT Transporter major facilitator family OS=Prevotella sp. CAG:924 OX=1262938 GN=BN812_00664 PE=4 SV=1
MM1 pKa = 8.18DD2 pKa = 4.4YY3 pKa = 11.08AVDD6 pKa = 3.86MPVGEE11 pKa = 4.56SLIADD16 pKa = 3.79GGEE19 pKa = 4.13PVDD22 pKa = 4.56SFLDD26 pKa = 4.37EE27 pKa = 4.38ILQCASDD34 pKa = 4.21NIEE37 pKa = 4.52SEE39 pKa = 4.87PEE41 pKa = 4.14DD42 pKa = 3.75EE43 pKa = 4.81DD44 pKa = 4.82HH45 pKa = 7.11NNQEE49 pKa = 4.26GRR51 pKa = 11.84DD52 pKa = 4.01GGPLACEE59 pKa = 4.02HH60 pKa = 6.22TVDD63 pKa = 4.52GAAACMLFALPGLYY77 pKa = 10.36DD78 pKa = 3.31RR79 pKa = 11.84VGAGFEE85 pKa = 4.27DD86 pKa = 4.17EE87 pKa = 5.17AVAHH91 pKa = 5.89VGNGGSRR98 pKa = 11.84VEE100 pKa = 4.23APLVLHH106 pKa = 6.64LLADD110 pKa = 4.38VIDD113 pKa = 3.96HH114 pKa = 6.56GFLIVFQLEE123 pKa = 4.47PFHH126 pKa = 6.63HH127 pKa = 5.9QQ128 pKa = 2.97

Molecular weight: 13.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5FC68|R5FC68_9BACT Ribosomal RNA small subunit methyltransferase E OS=Prevotella sp. CAG:924 OX=1262938 GN=BN812_02098 PE=3 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2225

0

2225

773435

29

1989

347.6

39.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.628 ± 0.05

1.299 ± 0.02

6.089 ± 0.036

5.795 ± 0.047

4.16 ± 0.029

7.077 ± 0.041

2.33 ± 0.027

6.365 ± 0.04

5.807 ± 0.045

9.072 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.907 ± 0.026

4.544 ± 0.048

3.85 ± 0.026

3.767 ± 0.033

5.232 ± 0.043

5.891 ± 0.041

5.906 ± 0.039

6.748 ± 0.039

1.277 ± 0.022

4.254 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski