Weissella phage PWc

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G1I5V1|A0A3G1I5V1_9CAUD Endopeptidase OS=Weissella phage PWc OX=1970788 GN=pwc_44 PE=4 SV=1
MM1 pKa = 7.46NNTIAFNNLVEE12 pKa = 4.35ALQFHH17 pKa = 6.54GAFNGVEE24 pKa = 4.1TPEE27 pKa = 4.33TVKK30 pKa = 11.03SFLKK34 pKa = 10.68YY35 pKa = 9.8VEE37 pKa = 4.84TYY39 pKa = 11.03VSLDD43 pKa = 3.24TVAAIEE49 pKa = 4.1NGEE52 pKa = 4.01FGVFGSLEE60 pKa = 3.89EE61 pKa = 4.35YY62 pKa = 10.4YY63 pKa = 10.88DD64 pKa = 3.8YY65 pKa = 11.69VADD68 pKa = 4.26FASDD72 pKa = 3.54EE73 pKa = 4.6DD74 pKa = 4.7LNCDD78 pKa = 4.77DD79 pKa = 5.91LLPEE83 pKa = 4.55PKK85 pKa = 10.27FPFANVWISVKK96 pKa = 10.7AA97 pKa = 3.55

Molecular weight: 10.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G1I5Y7|A0A3G1I5Y7_9CAUD Holin OS=Weissella phage PWc OX=1970788 GN=pwc_21 PE=4 SV=1
MM1 pKa = 7.51EE2 pKa = 5.27GSKK5 pKa = 10.56ALHH8 pKa = 6.24SRR10 pKa = 11.84FDD12 pKa = 3.49GLINIDD18 pKa = 3.36TRR20 pKa = 11.84VRR22 pKa = 11.84AIVRR26 pKa = 11.84NNADD30 pKa = 4.54DD31 pKa = 3.22ISARR35 pKa = 11.84TKK37 pKa = 11.0SNAPVDD43 pKa = 3.8TGKK46 pKa = 10.36LRR48 pKa = 11.84SGVNVSMSGSAHH60 pKa = 5.02QSKK63 pKa = 10.83AVITANAYY71 pKa = 10.4NKK73 pKa = 9.61GFNYY77 pKa = 10.51GLYY80 pKa = 8.66QEE82 pKa = 4.94RR83 pKa = 11.84YY84 pKa = 7.21NRR86 pKa = 11.84RR87 pKa = 11.84GKK89 pKa = 10.44SEE91 pKa = 3.6FMEE94 pKa = 4.26RR95 pKa = 11.84AFKK98 pKa = 10.68AVEE101 pKa = 3.91PKK103 pKa = 10.1FKK105 pKa = 10.74KK106 pKa = 10.7EE107 pKa = 3.51MMEE110 pKa = 4.64AISKK114 pKa = 10.16RR115 pKa = 3.58

Molecular weight: 12.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

11418

33

1008

211.4

23.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.006 ± 0.66

0.307 ± 0.072

6.507 ± 0.301

7.243 ± 0.465

3.81 ± 0.164

7.147 ± 0.465

1.419 ± 0.171

6.744 ± 0.258

7.348 ± 0.276

8.022 ± 0.318

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.978 ± 0.218

6.586 ± 0.316

2.846 ± 0.168

3.284 ± 0.218

3.626 ± 0.266

5.666 ± 0.242

6.446 ± 0.222

7.401 ± 0.245

1.27 ± 0.128

4.344 ± 0.336
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski