Candidatus Arcanobacter lacustris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Rickettsiales genera incertae sedis; Candidatus Arcanobacter

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 815 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F5MNV2|A0A0F5MNV2_9RICK Integrase core domain protein OS=Candidatus Arcanobacter lacustris OX=1607817 GN=SZ25_00678 PE=4 SV=1
MM1 pKa = 7.51TIISTIEE8 pKa = 3.78SLKK11 pKa = 11.35DD12 pKa = 3.23MFNGNCQTASLKK24 pKa = 9.99TKK26 pKa = 10.14LANGDD31 pKa = 3.17NGYY34 pKa = 10.11NICATEE40 pKa = 4.58CYY42 pKa = 9.83NKK44 pKa = 10.77DD45 pKa = 3.36GMSPDD50 pKa = 3.63QLNLIIDD57 pKa = 5.03AIKK60 pKa = 11.0VEE62 pKa = 4.81FPDD65 pKa = 5.28DD66 pKa = 4.23LDD68 pKa = 4.36SSTSSIFTSGMEE80 pKa = 4.43GEE82 pKa = 4.77FKK84 pKa = 10.88NNSGIMVSFLGNCAEE99 pKa = 4.25YY100 pKa = 11.24GLGIADD106 pKa = 4.02ASSEE110 pKa = 4.03GSVILNN116 pKa = 3.65

Molecular weight: 12.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F5MPG1|A0A0F5MPG1_9RICK Alanine--tRNA ligase OS=Candidatus Arcanobacter lacustris OX=1607817 GN=alaS PE=3 SV=1
MM1 pKa = 7.07STQSVPEE8 pKa = 4.09NKK10 pKa = 10.34GYY12 pKa = 8.22TATSAYY18 pKa = 9.49GAQATNKK25 pKa = 8.77PVFFRR30 pKa = 11.84RR31 pKa = 11.84PKK33 pKa = 9.96SCPLSDD39 pKa = 4.36VPSSDD44 pKa = 3.85INYY47 pKa = 10.22KK48 pKa = 10.26NIKK51 pKa = 9.78LLTRR55 pKa = 11.84FISEE59 pKa = 4.5GGRR62 pKa = 11.84ILPSRR67 pKa = 11.84ITSVSTSKK75 pKa = 10.33QRR77 pKa = 11.84EE78 pKa = 4.0LKK80 pKa = 10.5VAIKK84 pKa = 10.2RR85 pKa = 11.84ARR87 pKa = 11.84SLALLPFAQKK97 pKa = 10.8

Molecular weight: 10.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

815

0

815

227547

22

2622

279.2

31.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.736 ± 0.087

0.987 ± 0.036

5.763 ± 0.075

5.966 ± 0.107

4.32 ± 0.057

5.781 ± 0.093

1.92 ± 0.037

9.463 ± 0.087

7.954 ± 0.091

9.618 ± 0.101

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.048

6.427 ± 0.106

3.173 ± 0.065

3.241 ± 0.046

3.498 ± 0.066

7.743 ± 0.099

4.952 ± 0.085

5.587 ± 0.077

0.783 ± 0.029

3.668 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski