Hanseniaspora uvarum (Yeast) (Kloeckera apiculata)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4059 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1E5S1Z9|A0A1E5S1Z9_HANUV Ceramide synthase subunit LIP1 OS=Hanseniaspora uvarum OX=29833 GN=AWRI3580_g76 PE=4 SV=1
MM1 pKa = 7.59QYY3 pKa = 7.88TTITSIFLLTISLLTQSTSAIGTLGINVASSGVDD37 pKa = 3.5GNCRR41 pKa = 11.84DD42 pKa = 3.53SDD44 pKa = 3.72SWSEE48 pKa = 4.07ALEE51 pKa = 4.17KK52 pKa = 10.92ASAFTNYY59 pKa = 8.96IKK61 pKa = 10.0TYY63 pKa = 10.62AVSDD67 pKa = 4.0CNMLEE72 pKa = 4.27NIVPALNSNTNMKK85 pKa = 9.47VWMGVWEE92 pKa = 4.04VDD94 pKa = 3.35EE95 pKa = 4.64AHH97 pKa = 7.04FDD99 pKa = 3.72AEE101 pKa = 4.39KK102 pKa = 10.96DD103 pKa = 3.38ALTTYY108 pKa = 10.44LPSISKK114 pKa = 10.62DD115 pKa = 3.43SIAGISVGSEE125 pKa = 3.03ALYY128 pKa = 10.84RR129 pKa = 11.84GDD131 pKa = 4.37LTPQALASKK140 pKa = 9.91ISEE143 pKa = 4.13IKK145 pKa = 10.68SLLSDD150 pKa = 3.27ITDD153 pKa = 3.46KK154 pKa = 11.5DD155 pKa = 3.85GNSYY159 pKa = 11.03SGVSVGTVDD168 pKa = 4.59SWNIWVNGTNAPAIQAADD186 pKa = 3.9YY187 pKa = 11.22IMVNAFPFWQYY198 pKa = 7.92QTANNQSHH206 pKa = 6.35SLVDD210 pKa = 4.17DD211 pKa = 3.7VMQAVKK217 pKa = 10.51AIQEE221 pKa = 4.21AKK223 pKa = 9.59GTDD226 pKa = 3.3DD227 pKa = 4.77FYY229 pKa = 11.13ISVGEE234 pKa = 4.27TGAPTGGNEE243 pKa = 4.19LTSGQAPLTVDD254 pKa = 3.1NASDD258 pKa = 4.12LFQEE262 pKa = 5.73GICTLLAYY270 pKa = 10.29GIDD273 pKa = 3.66VNIFEE278 pKa = 5.22LQDD281 pKa = 3.54EE282 pKa = 4.58PTKK285 pKa = 10.67PLATADD291 pKa = 4.55DD292 pKa = 4.87GSSSDD297 pKa = 4.1VEE299 pKa = 4.44RR300 pKa = 11.84HH301 pKa = 4.36WGVYY305 pKa = 8.53DD306 pKa = 3.19TDD308 pKa = 4.08YY309 pKa = 11.28NLKK312 pKa = 10.61YY313 pKa = 10.76SLTCSYY319 pKa = 11.2GSNN322 pKa = 3.33

Molecular weight:
34.66 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1E5RJZ3|A0A1E5RJZ3_HANUV Cell division control protein 53 OS=Hanseniaspora uvarum OX=29833 GN=AWRI3580_g2889 PE=3 SV=1
MM1 pKa = 7.52DD2 pKa = 5.56TIRR5 pKa = 11.84TGITGKK11 pKa = 10.68YY12 pKa = 7.27GVRR15 pKa = 11.84YY16 pKa = 9.34GSSLRR21 pKa = 11.84RR22 pKa = 11.84QVKK25 pKa = 9.69KK26 pKa = 11.14LEE28 pKa = 4.21VQQHH32 pKa = 4.66SRR34 pKa = 11.84YY35 pKa = 10.26DD36 pKa = 3.35CSFCGKK42 pKa = 10.23KK43 pKa = 9.77SVKK46 pKa = 10.2RR47 pKa = 11.84GATGIWNCGSCKK59 pKa = 9.43KK60 pKa = 8.82TVAGGAYY67 pKa = 8.32TVSTAAAATVRR78 pKa = 11.84STIRR82 pKa = 11.84RR83 pKa = 11.84LRR85 pKa = 11.84DD86 pKa = 3.24MVEE89 pKa = 3.53AA90 pKa = 4.79

Molecular weight:
9.84 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4059

0

4059

2125948

66

4955

523.8

59.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.36 ± 0.036

1.123 ± 0.011

6.269 ± 0.03

6.933 ± 0.035

4.801 ± 0.025

4.332 ± 0.041

1.799 ± 0.012

7.475 ± 0.033

8.957 ± 0.044

9.61 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.126 ± 0.015

7.721 ± 0.048

3.361 ± 0.025

3.674 ± 0.022

3.08 ± 0.021

8.31 ± 0.052

5.523 ± 0.042

5.354 ± 0.023

0.822 ± 0.01

3.864 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski