Hydrogenophaga sp. IBVHS1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Hydrogenophaga; unclassified Hydrogenophaga

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4193 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y2R020|A0A1Y2R020_9BURK AI-2E family transporter OS=Hydrogenophaga sp. IBVHS1 OX=1985169 GN=CAP37_15745 PE=3 SV=1
MM1 pKa = 7.24KK2 pKa = 9.99HH3 pKa = 5.51QLIMMGLTLALTACGGGGDD22 pKa = 4.04EE23 pKa = 4.62MGEE26 pKa = 4.19GAPLQAQGLDD36 pKa = 3.03LAYY39 pKa = 10.38AVNTFDD45 pKa = 3.67HH46 pKa = 6.9TISSFTANGATGALSAVGPALPTGSGPAAVGVHH79 pKa = 5.05PAGRR83 pKa = 11.84FVYY86 pKa = 9.9VANFGGDD93 pKa = 3.36SVSAYY98 pKa = 10.46SVAINDD104 pKa = 4.09GALTPVGEE112 pKa = 4.67FAAGDD117 pKa = 3.95GPRR120 pKa = 11.84SVTVSRR126 pKa = 11.84DD127 pKa = 2.87GRR129 pKa = 11.84FVYY132 pKa = 10.14VANDD136 pKa = 3.44LSDD139 pKa = 4.74DD140 pKa = 3.64VSTYY144 pKa = 10.96AINAATGALNAVGLAVATGTNPRR167 pKa = 11.84SVVVDD172 pKa = 3.73PQTRR176 pKa = 11.84FAWVANLLSDD186 pKa = 3.83SVSAYY191 pKa = 9.24TINALTGALSPIGVPVAAGDD211 pKa = 3.88GPTALGIDD219 pKa = 3.45PTGRR223 pKa = 11.84FAYY226 pKa = 9.94VVNTNDD232 pKa = 3.56DD233 pKa = 3.97TVVSYY238 pKa = 10.97AIDD241 pKa = 3.64AVTGALSPLGAPVATGDD258 pKa = 3.14APAAVRR264 pKa = 11.84VHH266 pKa = 6.98PGGHH270 pKa = 5.36WLYY273 pKa = 10.69VANRR277 pKa = 11.84LSDD280 pKa = 3.87DD281 pKa = 3.65VSVYY285 pKa = 10.86AIDD288 pKa = 4.0PVSGQLSAVGVPVTAGDD305 pKa = 4.57GPQSMGVSPDD315 pKa = 2.68GRR317 pKa = 11.84FAYY320 pKa = 9.82VANATSDD327 pKa = 4.01DD328 pKa = 3.82VSSYY332 pKa = 11.27AIDD335 pKa = 3.96AGSGALIAVGPPAPAGDD352 pKa = 3.98GPVGIDD358 pKa = 3.32ATNTSLLLQQ367 pKa = 3.98

Molecular weight: 36.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y2QSY2|A0A1Y2QSY2_9BURK Lysine transporter LysE OS=Hydrogenophaga sp. IBVHS1 OX=1985169 GN=CAP37_20060 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.07IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4193

0

4193

1365467

37

3571

325.7

35.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.484 ± 0.049

0.932 ± 0.012

5.045 ± 0.029

5.346 ± 0.035

3.556 ± 0.024

8.365 ± 0.028

2.389 ± 0.02

4.201 ± 0.031

3.165 ± 0.033

10.929 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.64 ± 0.021

2.576 ± 0.019

5.34 ± 0.028

3.985 ± 0.025

6.917 ± 0.033

5.397 ± 0.022

5.23 ± 0.021

7.852 ± 0.029

1.565 ± 0.019

2.085 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski