Shayang ascaridia galli virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Betanemrhavirus; Shayang betanemrhavirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KN28|A0A1L3KN28_9VIRU Putative nucleoprotein OS=Shayang ascaridia galli virus 2 OX=1923460 PE=4 SV=1
MM1 pKa = 8.14DD2 pKa = 5.69SNDD5 pKa = 3.5TLLDD9 pKa = 3.95GNEE12 pKa = 3.91LQEE15 pKa = 4.69AEE17 pKa = 4.13HH18 pKa = 6.65HH19 pKa = 6.54KK20 pKa = 11.17NSLLALAQDD29 pKa = 3.87TNFVSKK35 pKa = 9.64YY36 pKa = 8.33TLTSGDD42 pKa = 3.62FADD45 pKa = 4.99TEE47 pKa = 4.31DD48 pKa = 4.98PYY50 pKa = 11.88DD51 pKa = 3.65DD52 pKa = 4.37TRR54 pKa = 11.84FQLPQTPTSEE64 pKa = 4.35STTTAASYY72 pKa = 9.93PPRR75 pKa = 11.84TYY77 pKa = 9.98ATSSSDD83 pKa = 3.08HH84 pKa = 6.92SDD86 pKa = 3.67DD87 pKa = 5.54LRR89 pKa = 11.84HH90 pKa = 6.53KK91 pKa = 10.11YY92 pKa = 10.04INEE95 pKa = 3.61AAAALHH101 pKa = 6.27KK102 pKa = 10.76AGIPIDD108 pKa = 4.41SINQFLYY115 pKa = 9.81THH117 pKa = 7.47DD118 pKa = 3.77KK119 pKa = 10.67NKK121 pKa = 10.5AGFWEE126 pKa = 5.01GVVQCANLFLTKK138 pKa = 10.32SSSQALDD145 pKa = 4.75AIPLLHH151 pKa = 6.84SKK153 pKa = 9.93IDD155 pKa = 3.72SLQKK159 pKa = 10.82QMANLCEE166 pKa = 4.24LLTTNDD172 pKa = 3.3KK173 pKa = 10.06LTVQNHH179 pKa = 5.23NKK181 pKa = 10.17EE182 pKa = 4.59SVQSQQHH189 pKa = 5.91SGIQPEE195 pKa = 4.53PKK197 pKa = 8.74PQSVTPLEE205 pKa = 4.11IKK207 pKa = 10.81FNDD210 pKa = 3.62TTPLYY215 pKa = 10.87ARR217 pKa = 11.84EE218 pKa = 4.13AVTNLPIASACKK230 pKa = 9.93QYY232 pKa = 11.13LADD235 pKa = 3.66CMARR239 pKa = 11.84EE240 pKa = 4.41FIPWSFLHH248 pKa = 7.15AIHH251 pKa = 6.73NATSPNMKK259 pKa = 8.42RR260 pKa = 11.84TSYY263 pKa = 11.44LEE265 pKa = 3.82MIEE268 pKa = 3.96YY269 pKa = 8.53MRR271 pKa = 11.84SHH273 pKa = 6.46GMPPDD278 pKa = 3.44VYY280 pKa = 11.48DD281 pKa = 5.04FFIKK285 pKa = 10.08PWNN288 pKa = 3.54

Molecular weight: 32.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KN19|A0A1L3KN19_9VIRU Uncharacterized protein OS=Shayang ascaridia galli virus 2 OX=1923460 PE=4 SV=1
MM1 pKa = 6.33TTYY4 pKa = 11.34LVMNMSGVIDD14 pKa = 3.85VSVAPPADD22 pKa = 3.47KK23 pKa = 11.19QEE25 pKa = 4.39TVDD28 pKa = 3.79IACQMVMAAITPPIPTEE45 pKa = 3.76LRR47 pKa = 11.84AAAARR52 pKa = 11.84ILCNKK57 pKa = 9.77LDD59 pKa = 3.76EE60 pKa = 4.69CRR62 pKa = 11.84PLQSTVITSNGKK74 pKa = 9.54VPGLRR79 pKa = 11.84YY80 pKa = 8.95PVLLDD85 pKa = 3.2GKK87 pKa = 10.32FAVKK91 pKa = 10.43LKK93 pKa = 10.43ADD95 pKa = 3.74RR96 pKa = 11.84PTRR99 pKa = 11.84DD100 pKa = 3.75FVSLHH105 pKa = 5.86CPQRR109 pKa = 11.84LDD111 pKa = 5.17NEE113 pKa = 4.52DD114 pKa = 4.37DD115 pKa = 3.88NNNSLFIHH123 pKa = 6.22LTLSAEE129 pKa = 4.02LGIINGVTANQLTITGRR146 pKa = 11.84HH147 pKa = 4.54IVPYY151 pKa = 10.05LGNDD155 pKa = 3.12EE156 pKa = 4.22RR157 pKa = 11.84FEE159 pKa = 5.23AIAAASIKK167 pKa = 10.33KK168 pKa = 10.46KK169 pKa = 10.57SDD171 pKa = 3.36TPKK174 pKa = 10.17SPIRR178 pKa = 11.84RR179 pKa = 11.84FAALFKK185 pKa = 10.74KK186 pKa = 10.39KK187 pKa = 9.85NHH189 pKa = 5.64NN190 pKa = 3.5

Molecular weight: 20.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4061

190

2222

676.8

75.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.535 ± 0.636

2.241 ± 0.38

5.27 ± 0.417

5.319 ± 0.307

4.186 ± 0.376

4.432 ± 0.294

2.339 ± 0.31

5.639 ± 0.509

5.097 ± 0.281

10.81 ± 0.965

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.413 ± 0.192

4.26 ± 0.582

5.023 ± 0.345

3.644 ± 0.468

5.294 ± 0.542

8.422 ± 0.39

6.722 ± 0.746

6.846 ± 0.578

1.551 ± 0.288

2.955 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski