Klebsiella phage ST512-KPC3phi13.2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Felsduovirus; Klebsiella virus ST512KPC3phi13-2

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482J511|A0A482J511_9CAUD TraR/DksA family transcriptional regulator OS=Klebsiella phage ST512-KPC3phi13.2 OX=2510470 PE=4 SV=1
MM1 pKa = 6.37TTVEE5 pKa = 3.99VRR7 pKa = 11.84IEE9 pKa = 4.21TVNGSMVTFSRR20 pKa = 11.84VSEE23 pKa = 3.84NWVNLNQYY31 pKa = 9.35EE32 pKa = 4.04RR33 pKa = 11.84DD34 pKa = 4.09DD35 pKa = 5.4IISGWINEE43 pKa = 4.59DD44 pKa = 3.6KK45 pKa = 10.98NSQAALSASDD55 pKa = 5.03GYY57 pKa = 9.03TLSYY61 pKa = 10.46HH62 pKa = 6.46VLAQEE67 pKa = 4.01

Molecular weight: 7.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482J6R5|A0A482J6R5_9CAUD Tail protein I OS=Klebsiella phage ST512-KPC3phi13.2 OX=2510470 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.24KK3 pKa = 10.29KK4 pKa = 10.81LIGGLFSVLYY14 pKa = 7.6TALMIFSLFVPNNIVPALVTALTWIACLLSWGAVLLCLAGWYY56 pKa = 10.28AGGTHH61 pKa = 6.45RR62 pKa = 11.84RR63 pKa = 11.84EE64 pKa = 4.16AKK66 pKa = 9.52QALTRR71 pKa = 11.84FFSTPGNQVIRR82 pKa = 11.84WARR85 pKa = 11.84CSLLVIFLPFTGHH98 pKa = 5.45VVTLVFYY105 pKa = 11.01LLTLAALKK113 pKa = 10.33VLRR116 pKa = 11.84AQIIDD121 pKa = 3.88AEE123 pKa = 4.41PVTVV127 pKa = 3.76

Molecular weight: 14.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

10205

39

983

231.9

25.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.848 ± 0.485

1.039 ± 0.157

5.772 ± 0.26

6.056 ± 0.292

3.332 ± 0.237

7.006 ± 0.475

1.793 ± 0.181

5.429 ± 0.32

5.047 ± 0.307

9.241 ± 0.388

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.754 ± 0.205

4.802 ± 0.3

4.047 ± 0.176

4.165 ± 0.271

6.438 ± 0.357

6.232 ± 0.245

5.86 ± 0.404

6.575 ± 0.334

1.48 ± 0.129

3.087 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski