Beet yellows virus (isolate Ukraine) (BYV) (Sugar beet yellows virus)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Closterovirus; Beet yellows virus

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q08545|P21_BYVU RNA silencing suppressor OS=Beet yellows virus (isolate Ukraine) OX=478555 GN=ORF8 PE=1 SV=1
MM1 pKa = 6.97TSSVEE6 pKa = 3.95LAQTKK11 pKa = 9.81PLFRR15 pKa = 11.84VLLLKK20 pKa = 10.92GFVFYY25 pKa = 10.79VVAFEE30 pKa = 4.39TEE32 pKa = 4.37EE33 pKa = 4.1EE34 pKa = 4.24SSEE37 pKa = 4.35AEE39 pKa = 3.95LPLVYY44 pKa = 10.72LHH46 pKa = 7.47DD47 pKa = 4.33FEE49 pKa = 6.69LNINKK54 pKa = 9.81RR55 pKa = 11.84GKK57 pKa = 9.85IEE59 pKa = 3.79ASYY62 pKa = 11.13VDD64 pKa = 5.67FMSCMTRR71 pKa = 11.84LKK73 pKa = 9.99PSSVSYY79 pKa = 10.21TRR81 pKa = 11.84VSSEE85 pKa = 3.69KK86 pKa = 10.31SSEE89 pKa = 4.06DD90 pKa = 3.27FSLPGSGKK98 pKa = 7.96TFGSKK103 pKa = 9.51VLNRR107 pKa = 11.84KK108 pKa = 6.97VTFTFEE114 pKa = 4.07NGVQLVFGMYY124 pKa = 10.31GLEE127 pKa = 3.95QRR129 pKa = 11.84CVSSDD134 pKa = 3.55YY135 pKa = 11.49LWFEE139 pKa = 4.02NVFVGAHH146 pKa = 6.53CGTLTYY152 pKa = 10.68CLNCEE157 pKa = 4.01LDD159 pKa = 3.34KK160 pKa = 11.67SGGEE164 pKa = 3.97LEE166 pKa = 4.36ILTFSKK172 pKa = 11.36NEE174 pKa = 3.77VLLKK178 pKa = 10.47RR179 pKa = 11.84WW180 pKa = 3.27

Molecular weight: 20.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q08544|P20_BYVU 20 kDa protein OS=Beet yellows virus (isolate Ukraine) OX=478555 GN=ORF7 PE=1 SV=1
MM1 pKa = 7.79DD2 pKa = 4.88CVLRR6 pKa = 11.84SYY8 pKa = 11.44LLLAFGFLICLFLFCLVVFIWFVYY32 pKa = 9.61KK33 pKa = 10.61QILFRR38 pKa = 11.84TTAQSNEE45 pKa = 3.39ARR47 pKa = 11.84HH48 pKa = 5.17NHH50 pKa = 4.61STVVV54 pKa = 3.06

Molecular weight: 6.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

1

9

7706

54

3094

856.2

96.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.411 ± 0.317

2.44 ± 0.121

4.672 ± 0.173

6.761 ± 0.312

6.268 ± 0.256

5.022 ± 0.426

1.778 ± 0.12

4.01 ± 0.181

5.84 ± 0.249

10.213 ± 0.436

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.44 ± 0.142

3.867 ± 0.281

4.127 ± 0.27

1.869 ± 0.1

6.852 ± 0.448

10.433 ± 0.482

5.165 ± 0.156

8.798 ± 0.437

0.558 ± 0.09

3.478 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski