Cyclovirus PKbeef23/PAK/2009

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Cyclovirus; Bovine associated cyclovirus 1

Average proteome isoelectric point is 8.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9NWS3|E9NWS3_9CIRC Cap protein OS=Cyclovirus PKbeef23/PAK/2009 OX=942032 PE=4 SV=1
MM1 pKa = 7.72APNKK5 pKa = 7.84TLRR8 pKa = 11.84RR9 pKa = 11.84FCWTLNNYY17 pKa = 8.59TEE19 pKa = 5.48DD20 pKa = 5.87DD21 pKa = 3.54VDD23 pKa = 4.17QLQKK27 pKa = 10.95DD28 pKa = 3.9LPEE31 pKa = 4.39LCKK34 pKa = 9.8FTIFGRR40 pKa = 11.84EE41 pKa = 3.84VCPTTGTKK49 pKa = 9.98HH50 pKa = 6.08LQGFCNLQRR59 pKa = 11.84PKK61 pKa = 10.52RR62 pKa = 11.84FNNIRR67 pKa = 11.84EE68 pKa = 4.13IFKK71 pKa = 10.75GRR73 pKa = 11.84AHH75 pKa = 6.87IEE77 pKa = 3.89GAKK80 pKa = 10.74GSDD83 pKa = 3.17QDD85 pKa = 3.65NQRR88 pKa = 11.84YY89 pKa = 8.69CSKK92 pKa = 10.76SGEE95 pKa = 4.33VWSHH99 pKa = 6.61GEE101 pKa = 3.77PCNQGARR108 pKa = 11.84SDD110 pKa = 3.94LEE112 pKa = 4.17EE113 pKa = 3.9VVSIIKK119 pKa = 10.58GGEE122 pKa = 3.66RR123 pKa = 11.84DD124 pKa = 3.71VKK126 pKa = 10.78AVALQCPTAYY136 pKa = 9.32IKK138 pKa = 10.61YY139 pKa = 9.99FKK141 pKa = 10.74GIEE144 pKa = 3.85NYY146 pKa = 9.58IRR148 pKa = 11.84IYY150 pKa = 10.49HH151 pKa = 6.02ATPEE155 pKa = 4.28RR156 pKa = 11.84DD157 pKa = 3.5FKK159 pKa = 10.88TEE161 pKa = 4.14VYY163 pKa = 9.81FFWGPTGAGKK173 pKa = 10.39SRR175 pKa = 11.84TARR178 pKa = 11.84EE179 pKa = 3.66QALATGLRR187 pKa = 11.84VYY189 pKa = 9.71YY190 pKa = 10.2KK191 pKa = 10.53PRR193 pKa = 11.84GDD195 pKa = 2.71WWDD198 pKa = 3.59GYY200 pKa = 11.08NGHH203 pKa = 6.6EE204 pKa = 4.56CVIFDD209 pKa = 5.69DD210 pKa = 4.91FYY212 pKa = 11.68GWIKK216 pKa = 10.41YY217 pKa = 10.54DD218 pKa = 3.92EE219 pKa = 4.17VLKK222 pKa = 10.89ICDD225 pKa = 3.61RR226 pKa = 11.84YY227 pKa = 9.9PYY229 pKa = 9.86RR230 pKa = 11.84VPVKK234 pKa = 10.28GGYY237 pKa = 9.69EE238 pKa = 3.6NFIAKK243 pKa = 9.92KK244 pKa = 9.87IWFTSNKK251 pKa = 9.17PLEE254 pKa = 4.31QIYY257 pKa = 10.49KK258 pKa = 10.23FIDD261 pKa = 3.61YY262 pKa = 9.7EE263 pKa = 3.92PSAWRR268 pKa = 11.84RR269 pKa = 11.84RR270 pKa = 11.84LTVEE274 pKa = 4.09RR275 pKa = 11.84EE276 pKa = 3.92FFYY279 pKa = 10.81EE280 pKa = 3.92

Molecular weight: 32.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E9NWS3|E9NWS3_9CIRC Cap protein OS=Cyclovirus PKbeef23/PAK/2009 OX=942032 PE=4 SV=1
MM1 pKa = 7.84RR2 pKa = 11.84APIRR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 9.36RR9 pKa = 11.84RR10 pKa = 11.84AIRR13 pKa = 11.84HH14 pKa = 3.72VRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84LRR20 pKa = 11.84KK21 pKa = 7.96VRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84QRR27 pKa = 11.84TGNITVSMKK36 pKa = 10.25SLYY39 pKa = 9.31VAPVEE44 pKa = 4.43GKK46 pKa = 8.28TGYY49 pKa = 10.54LLQISPAVSDD59 pKa = 3.68FTEE62 pKa = 3.83AATFINYY69 pKa = 9.37FEE71 pKa = 5.39AYY73 pKa = 9.75RR74 pKa = 11.84IWSVKK79 pKa = 9.94VKK81 pKa = 10.48VNPLFNVAADD91 pKa = 3.81TTPVPRR97 pKa = 11.84YY98 pKa = 10.02YY99 pKa = 10.49SAPWHH104 pKa = 6.37KK105 pKa = 10.45PGPTSINSTGIVTIDD120 pKa = 2.95KK121 pKa = 10.07CKK123 pKa = 10.6SYY125 pKa = 11.22NGLSGSVRR133 pKa = 11.84RR134 pKa = 11.84FVPAVLTATGYY145 pKa = 11.3AGITTPQYY153 pKa = 11.67GKK155 pKa = 10.43INWRR159 pKa = 11.84PRR161 pKa = 11.84IEE163 pKa = 4.69LNSNTATLPHH173 pKa = 6.27YY174 pKa = 10.42CGLYY178 pKa = 9.91LWSADD183 pKa = 3.5QQEE186 pKa = 4.92GSTHH190 pKa = 4.86IRR192 pKa = 11.84QYY194 pKa = 10.76EE195 pKa = 3.91IEE197 pKa = 4.22TEE199 pKa = 4.16IKK201 pKa = 8.94VTFYY205 pKa = 10.65NQKK208 pKa = 10.49SFLGG212 pKa = 3.8

Molecular weight: 24.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

492

212

280

246.0

28.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.894 ± 0.68

2.236 ± 0.744

4.065 ± 1.254

6.098 ± 1.337

4.878 ± 0.907

7.114 ± 0.565

1.829 ± 0.033

6.504 ± 0.329

6.911 ± 0.719

5.285 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.61 ± 0.192

4.472 ± 0.141

5.285 ± 0.488

3.455 ± 0.088

8.74 ± 0.671

4.878 ± 1.265

6.911 ± 1.181

6.098 ± 0.834

2.439 ± 0.318

6.301 ± 0.097
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski