Rothia nasimurium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Rothia

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2215 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y1RQD3|A0A1Y1RQD3_9MICC Phosphate starvation-inducible protein PhoH OS=Rothia nasimurium OX=85336 GN=A7979_00330 PE=4 SV=1
MM1 pKa = 7.75SINITVTDD9 pKa = 3.91EE10 pKa = 3.92NGTTKK15 pKa = 10.78DD16 pKa = 3.78VTWEE20 pKa = 3.79PHH22 pKa = 4.26QSMLEE27 pKa = 3.96AVLEE31 pKa = 4.34AGFPVLATCGGNASCATCHH50 pKa = 6.71AFIDD54 pKa = 4.51PDD56 pKa = 5.43HH57 pKa = 7.32IDD59 pKa = 2.99ASLPRR64 pKa = 11.84EE65 pKa = 4.06EE66 pKa = 5.49AEE68 pKa = 4.28EE69 pKa = 4.61DD70 pKa = 3.87LLDD73 pKa = 4.79MIDD76 pKa = 5.56DD77 pKa = 4.03IANEE81 pKa = 4.32CSRR84 pKa = 11.84LSCQTEE90 pKa = 4.04YY91 pKa = 11.34SEE93 pKa = 4.56GMNGAKK99 pKa = 8.64VTLQPGMM106 pKa = 4.12

Molecular weight: 11.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y1RRN7|A0A1Y1RRN7_9MICC Uncharacterized protein OS=Rothia nasimurium OX=85336 GN=A7979_10045 PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 9.98VRR4 pKa = 11.84NSIRR8 pKa = 11.84SLKK11 pKa = 10.04KK12 pKa = 8.65MPGAQVVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84VFVINKK30 pKa = 8.84KK31 pKa = 9.21NPRR34 pKa = 11.84FKK36 pKa = 10.85ARR38 pKa = 11.84QGG40 pKa = 3.28

Molecular weight: 4.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2215

0

2215

743213

28

3359

335.5

36.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.915 ± 0.065

0.599 ± 0.012

5.282 ± 0.035

6.302 ± 0.049

3.392 ± 0.031

8.375 ± 0.051

2.082 ± 0.023

4.978 ± 0.044

3.212 ± 0.04

10.238 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.095 ± 0.021

2.858 ± 0.033

4.904 ± 0.032

3.553 ± 0.032

5.83 ± 0.044

6.058 ± 0.035

6.505 ± 0.035

7.927 ± 0.048

1.331 ± 0.021

2.563 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski