Sphingorhabdus sp. SMR4y

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingorhabdus; unclassified Sphingorhabdus

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3323 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A220W6R4|A0A220W6R4_9SPHN Glycerol-3-phosphate dehydrogenase [NAD(P)+] OS=Sphingorhabdus sp. SMR4y OX=2584094 GN=gpsA PE=3 SV=1
MM1 pKa = 7.4VLPILLIFLLGMMDD15 pKa = 3.76VGRR18 pKa = 11.84LMWDD22 pKa = 3.01WNRR25 pKa = 11.84AEE27 pKa = 5.35KK28 pKa = 8.76ATQMGVRR35 pKa = 11.84YY36 pKa = 9.39AVATDD41 pKa = 3.76IVPSALEE48 pKa = 4.2SYY50 pKa = 10.75SFAISGSVLQGDD62 pKa = 4.76PVPEE66 pKa = 4.0TAFPGIVCEE75 pKa = 4.49APGGTPACSCVTGGTCDD92 pKa = 3.07SAYY95 pKa = 10.83LSTVNATAFNNIVDD109 pKa = 5.31RR110 pKa = 11.84INLFKK115 pKa = 10.67PDD117 pKa = 3.29VAAEE121 pKa = 4.15NVVVEE126 pKa = 4.19YY127 pKa = 10.3GYY129 pKa = 11.11SGLGFAGDD137 pKa = 3.89PNGPDD142 pKa = 3.22VAPLVTIRR150 pKa = 11.84LQNLDD155 pKa = 3.95FQPLLTQIFGTTITLPDD172 pKa = 4.58FSAALTLEE180 pKa = 4.72DD181 pKa = 5.35GEE183 pKa = 4.58GTYY186 pKa = 11.37ANN188 pKa = 4.19

Molecular weight: 19.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A220W1Y8|A0A220W1Y8_9SPHN Carboxylesterase NlhH OS=Sphingorhabdus sp. SMR4y OX=2584094 GN=nlhH PE=4 SV=1
MM1 pKa = 8.18RR2 pKa = 11.84ILRR5 pKa = 11.84PSNQIVSPSTTQVTRR20 pKa = 11.84SGPKK24 pKa = 9.91QMPKK28 pKa = 10.21LARR31 pKa = 11.84FGSMAVAALVVAGPPLKK48 pKa = 10.67KK49 pKa = 9.02ITRR52 pKa = 11.84NSAVHH57 pKa = 6.17RR58 pKa = 11.84PAVRR62 pKa = 11.84MMKK65 pKa = 9.49WGQRR69 pKa = 11.84TVFTGWKK76 pKa = 8.17IAGRR80 pKa = 11.84GG81 pKa = 3.4

Molecular weight: 8.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3323

0

3323

1041894

30

4159

313.5

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.424 ± 0.055

0.845 ± 0.015

6.362 ± 0.037

6.047 ± 0.042

3.81 ± 0.027

8.473 ± 0.049

1.945 ± 0.022

5.911 ± 0.029

3.992 ± 0.039

9.54 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.681 ± 0.024

3.146 ± 0.027

4.837 ± 0.028

3.412 ± 0.024

6.164 ± 0.041

5.946 ± 0.034

5.118 ± 0.035

6.6 ± 0.034

1.345 ± 0.019

2.401 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski