Pseudomonas oryzae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1S471|A0A1H1S471_9PSED 3-oxoacyl-[acyl-carrier-protein] synthase I OS=Pseudomonas oryzae OX=1392877 GN=SAMN05216221_1799 PE=3 SV=1
MM1 pKa = 7.1HH2 pKa = 6.51THH4 pKa = 6.67KK5 pKa = 10.55FLRR8 pKa = 11.84TLPRR12 pKa = 11.84SLLSAAVVAGIVTAAGVFAVDD33 pKa = 4.25GNPPGLFEE41 pKa = 6.54LDD43 pKa = 3.62GNTVDD48 pKa = 4.59VGGTPGDD55 pKa = 3.47DD56 pKa = 2.94WGSIFTGTNLGTPAASTGILADD78 pKa = 3.92PAPLTIYY85 pKa = 8.81TQGGSKK91 pKa = 10.42DD92 pKa = 3.56INDD95 pKa = 3.38VSEE98 pKa = 3.88WRR100 pKa = 11.84HH101 pKa = 4.73TNGNVPDD108 pKa = 4.94KK109 pKa = 11.4DD110 pKa = 4.92DD111 pKa = 3.37ITNAYY116 pKa = 8.52AAGYY120 pKa = 9.33INPNNVSHH128 pKa = 6.84NGQVVHH134 pKa = 5.96QAGDD138 pKa = 3.86LIIYY142 pKa = 9.61FGLDD146 pKa = 2.75RR147 pKa = 11.84YY148 pKa = 10.89ANNGDD153 pKa = 3.39AFAGFWFFQDD163 pKa = 4.93DD164 pKa = 3.84VGLGPNGTFQGVHH177 pKa = 5.1TARR180 pKa = 11.84DD181 pKa = 3.33GDD183 pKa = 3.61QRR185 pKa = 11.84GDD187 pKa = 3.57LLVLVEE193 pKa = 4.33YY194 pKa = 9.55PQGANAQPEE203 pKa = 4.43IKK205 pKa = 10.47VYY207 pKa = 10.24EE208 pKa = 4.34WDD210 pKa = 4.29PADD213 pKa = 4.25ADD215 pKa = 3.89NDD217 pKa = 3.8NVADD221 pKa = 4.26NLDD224 pKa = 4.28EE225 pKa = 5.12IYY227 pKa = 11.03SSTNAKK233 pKa = 10.11CDD235 pKa = 3.33GAGNKK240 pKa = 8.27LACAITNVNNLTNEE254 pKa = 4.27PAWDD258 pKa = 3.89YY259 pKa = 10.52TPKK262 pKa = 10.54SGPASDD268 pKa = 4.41LPKK271 pKa = 10.64EE272 pKa = 4.32SFFEE276 pKa = 4.09GGINVTRR283 pKa = 11.84LLGGATPCFSSFLAEE298 pKa = 4.17TRR300 pKa = 11.84SSRR303 pKa = 11.84SEE305 pKa = 3.7TAQLKK310 pKa = 10.88DD311 pKa = 3.67FVLGNFDD318 pKa = 3.78LCSIAVTKK326 pKa = 9.93TCEE329 pKa = 4.6AEE331 pKa = 4.83VNDD334 pKa = 3.72QDD336 pKa = 3.84GGNSVVVNFSGEE348 pKa = 4.04VTNDD352 pKa = 2.85GGLALNNVTVTDD364 pKa = 4.19DD365 pKa = 3.3MGTPGDD371 pKa = 3.82TGDD374 pKa = 3.86DD375 pKa = 3.19QVVFGPATLQPGEE388 pKa = 4.15TQPYY392 pKa = 7.61SGSYY396 pKa = 7.34EE397 pKa = 4.04TTAIPATDD405 pKa = 3.29QVTATGSRR413 pKa = 11.84GTATVEE419 pKa = 3.74ATADD423 pKa = 3.77ATCSPTVSPAITVDD437 pKa = 3.84KK438 pKa = 10.7FCSATLKK445 pKa = 10.65EE446 pKa = 4.85DD447 pKa = 3.36GSGVDD452 pKa = 3.32VLFNGTVTNTGNVALEE468 pKa = 4.17NVTVVDD474 pKa = 4.83DD475 pKa = 5.1NGTADD480 pKa = 4.16PGDD483 pKa = 4.23DD484 pKa = 3.66VTVLGPVTLNAGASMPYY501 pKa = 9.96NGGFGVTGSNTSTDD515 pKa = 3.33HH516 pKa = 6.03VVASGTDD523 pKa = 3.16VLTDD527 pKa = 3.46VEE529 pKa = 4.53VSDD532 pKa = 4.03TAEE535 pKa = 4.17ATCLADD541 pKa = 3.71TNPSILLAKK550 pKa = 10.12QCTATVNSSGDD561 pKa = 3.71GIDD564 pKa = 3.41VSFTGSVTNNGNVILNNVTVIDD586 pKa = 4.68DD587 pKa = 3.78NGTPGNTGDD596 pKa = 5.53DD597 pKa = 3.38VTLIDD602 pKa = 4.01NATLAVGASLPFSGNFSGSGSSSTDD627 pKa = 3.16VVSATASDD635 pKa = 4.04ALTGDD640 pKa = 3.8VVSDD644 pKa = 3.89TEE646 pKa = 4.39TATCAADD653 pKa = 3.57VNPAILVSKK662 pKa = 10.59QCTATVNSAGTAIDD676 pKa = 3.41VLFIGSVTNTGNVVLQNVNVVDD698 pKa = 5.08DD699 pKa = 4.64NGTPGNSGDD708 pKa = 4.32DD709 pKa = 3.38VTLISNATLGVGASLPFNGNFSSSSASSTDD739 pKa = 3.12VVTATGVDD747 pKa = 3.7MLTSQAVSDD756 pKa = 4.37TEE758 pKa = 4.41TASCAADD765 pKa = 4.24LNPSIAVTKK774 pKa = 10.5QCTDD778 pKa = 2.94AMAHH782 pKa = 6.0DD783 pKa = 4.19QPILFNGTVSNTGNVALLGVTVVDD807 pKa = 5.25DD808 pKa = 4.43NGTPADD814 pKa = 4.38PLDD817 pKa = 4.21DD818 pKa = 3.57QTFNLGDD825 pKa = 4.06LAPGASANYY834 pKa = 9.1NGSYY838 pKa = 10.69SPGTGTWTNTVEE850 pKa = 4.32ANANGALEE858 pKa = 4.48TGSFTATASATCDD871 pKa = 3.52VPPPPPEE878 pKa = 4.48FEE880 pKa = 4.29GCTPGFWKK888 pKa = 10.5NSPGSWVGYY897 pKa = 9.83SPNQLVGSVFSLPSGVLNNQLGDD920 pKa = 3.88DD921 pKa = 4.11TLMEE925 pKa = 4.51ALGYY929 pKa = 9.81PGGTNLVGAAQILLRR944 pKa = 11.84AAVASLLNAGHH955 pKa = 7.48PDD957 pKa = 2.91VDD959 pKa = 4.67FPLTTSEE966 pKa = 5.74VITQVNAALATKK978 pKa = 10.55DD979 pKa = 3.17RR980 pKa = 11.84GTILALASTLDD991 pKa = 3.58GYY993 pKa = 12.05NNLGCDD999 pKa = 4.05LPNDD1003 pKa = 3.53NSFF1006 pKa = 3.53

Molecular weight: 102.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H1XPY9|A0A1H1XPY9_9PSED Beta sliding clamp OS=Pseudomonas oryzae OX=1392877 GN=SAMN05216221_3494 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.08RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 9.11RR41 pKa = 11.84LTTVV45 pKa = 2.62

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4103

0

4103

1362556

29

5777

332.1

36.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.221 ± 0.054

1.09 ± 0.014

5.259 ± 0.034

6.101 ± 0.04

3.457 ± 0.023

8.345 ± 0.047

2.31 ± 0.022

4.326 ± 0.029

2.879 ± 0.035

12.352 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.138 ± 0.022

2.56 ± 0.027

4.988 ± 0.028

4.359 ± 0.033

7.193 ± 0.048

5.273 ± 0.034

4.312 ± 0.05

7.005 ± 0.034

1.424 ± 0.016

2.407 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski