Eubacterium sp. AM18-10LB-B

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; unclassified Eubacterium

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2530 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A396KTW2|A0A396KTW2_9FIRM Uncharacterized protein OS=Eubacterium sp. AM18-10LB-B OX=2292044 GN=DW212_05125 PE=4 SV=1
MM1 pKa = 8.3DD2 pKa = 5.65DD3 pKa = 3.5MQVYY7 pKa = 9.61IANLGKK13 pKa = 10.45YY14 pKa = 9.93NEE16 pKa = 4.62GEE18 pKa = 4.02LVGAWFTFPIDD29 pKa = 3.62FEE31 pKa = 4.44EE32 pKa = 4.38VKK34 pKa = 10.79EE35 pKa = 4.14KK36 pKa = 10.6IGLNDD41 pKa = 3.31EE42 pKa = 4.29YY43 pKa = 11.02EE44 pKa = 4.27EE45 pKa = 4.26YY46 pKa = 10.57AIHH49 pKa = 7.92DD50 pKa = 4.17YY51 pKa = 10.78EE52 pKa = 5.43LPFTVDD58 pKa = 3.5EE59 pKa = 4.3YY60 pKa = 11.25TSIGEE65 pKa = 4.27LNRR68 pKa = 11.84LWEE71 pKa = 4.42MVSEE75 pKa = 4.35LPEE78 pKa = 4.12EE79 pKa = 4.32LQSEE83 pKa = 4.63LSALLTHH90 pKa = 6.76FSSIEE95 pKa = 3.86EE96 pKa = 4.05LSEE99 pKa = 3.91HH100 pKa = 6.0QEE102 pKa = 4.27DD103 pKa = 4.63IIIHH107 pKa = 5.68SDD109 pKa = 3.45CDD111 pKa = 3.6DD112 pKa = 3.81MYY114 pKa = 11.76DD115 pKa = 3.26VARR118 pKa = 11.84YY119 pKa = 9.56YY120 pKa = 10.1IEE122 pKa = 3.87EE123 pKa = 4.15TGALGEE129 pKa = 4.59VPASLQNYY137 pKa = 9.12IDD139 pKa = 3.66YY140 pKa = 10.5QAYY143 pKa = 9.95GRR145 pKa = 11.84DD146 pKa = 3.72LDD148 pKa = 4.23LSGTFISTNHH158 pKa = 6.71GIFEE162 pKa = 4.09IVYY165 pKa = 9.85

Molecular weight: 19.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A396KNP2|A0A396KNP2_9FIRM LysR family transcriptional regulator OS=Eubacterium sp. AM18-10LB-B OX=2292044 GN=DW212_05400 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 10.05RR10 pKa = 11.84KK11 pKa = 8.47HH12 pKa = 5.31QKK14 pKa = 6.82THH16 pKa = 4.76GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 8.95VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.59GRR39 pKa = 11.84KK40 pKa = 8.44VLSAA44 pKa = 4.05

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2530

0

2530

753616

23

2662

297.9

33.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.069 ± 0.049

1.493 ± 0.025

5.622 ± 0.041

7.65 ± 0.061

4.437 ± 0.04

6.013 ± 0.05

2.093 ± 0.025

8.095 ± 0.051

8.355 ± 0.052

9.336 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.195 ± 0.026

4.928 ± 0.039

2.835 ± 0.028

3.481 ± 0.034

3.64 ± 0.036

6.005 ± 0.04

4.962 ± 0.044

6.521 ± 0.04

0.82 ± 0.017

4.449 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski