Pseudomonas phage VSW-3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Napahaivirus; Pseudomonas virus VSW3

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A173GCN3|A0A173GCN3_9CAUD Uncharacterized protein OS=Pseudomonas phage VSW-3 OX=1852562 GN=VSW3_10 PE=4 SV=1
MM1 pKa = 7.47CPSFRR6 pKa = 11.84SFVAILCVLVAALSILMLQGCTVNVVYY33 pKa = 10.28MDD35 pKa = 4.23GVNMDD40 pKa = 4.01SSQNPYY46 pKa = 11.01NYY48 pKa = 9.49IEE50 pKa = 5.13APLATDD56 pKa = 4.13PMYY59 pKa = 11.29DD60 pKa = 3.98CDD62 pKa = 4.07FAPAADD68 pKa = 3.87EE69 pKa = 4.34CQQ71 pKa = 3.13

Molecular weight: 7.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A173GCY8|A0A173GCY8_9CAUD Uncharacterized protein OS=Pseudomonas phage VSW-3 OX=1852562 GN=VSW3_9 PE=4 SV=1
MM1 pKa = 7.55IARR4 pKa = 11.84TFNHH8 pKa = 5.41GHH10 pKa = 5.45VAMGSTLSITNGVTAEE26 pKa = 3.85VRR28 pKa = 11.84ALILEE33 pKa = 4.1RR34 pKa = 11.84EE35 pKa = 4.49GRR37 pKa = 11.84DD38 pKa = 3.14KK39 pKa = 10.87EE40 pKa = 3.89RR41 pKa = 11.84RR42 pKa = 11.84KK43 pKa = 10.31AYY45 pKa = 10.02RR46 pKa = 11.84KK47 pKa = 10.22SPTIRR52 pKa = 11.84PGSSRR57 pKa = 11.84DD58 pKa = 3.33TFKK61 pKa = 11.39DD62 pKa = 3.71FTPPTYY68 pKa = 10.53LAA70 pKa = 4.56

Molecular weight: 7.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

12492

37

1193

271.6

29.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.879 ± 0.461

0.993 ± 0.169

6.156 ± 0.174

5.828 ± 0.349

3.146 ± 0.172

8.069 ± 0.374

2.057 ± 0.167

4.891 ± 0.23

5.66 ± 0.462

8.125 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.082 ± 0.215

4.019 ± 0.276

4.187 ± 0.186

4.363 ± 0.308

5.804 ± 0.39

5.628 ± 0.237

5.932 ± 0.295

6.932 ± 0.266

1.313 ± 0.138

2.938 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski