Proteus phage vB_PmiP_RS10pmA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gorganvirus; unclassified Gorganvirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A517YZV7|A0A517YZV7_9CAUD Uncharacterized protein OS=Proteus phage vB_PmiP_RS10pmA OX=2250311 PE=4 SV=1
MM1 pKa = 7.22KK2 pKa = 10.47VKK4 pKa = 10.55FISATTHH11 pKa = 4.39VFTYY15 pKa = 10.0GKK17 pKa = 9.39EE18 pKa = 4.05YY19 pKa = 10.6EE20 pKa = 4.32MKK22 pKa = 10.71DD23 pKa = 3.84IGDD26 pKa = 4.44CYY28 pKa = 11.25AGFDD32 pKa = 3.83DD33 pKa = 5.72NGYY36 pKa = 9.88QFYY39 pKa = 10.33SWDD42 pKa = 3.34MGKK45 pKa = 10.38FGEE48 pKa = 4.27WEE50 pKa = 4.25IISEE54 pKa = 4.25

Molecular weight: 6.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A517YZV0|A0A517YZV0_9CAUD Uncharacterized protein OS=Proteus phage vB_PmiP_RS10pmA OX=2250311 PE=4 SV=1
MM1 pKa = 7.38SIKK4 pKa = 10.78VIVTKK9 pKa = 10.18PFYY12 pKa = 10.37ISLDD16 pKa = 2.91SWAIGSRR23 pKa = 11.84IDD25 pKa = 4.9KK26 pKa = 10.32IVSHH30 pKa = 6.8KK31 pKa = 10.6GRR33 pKa = 11.84WLVQTVPFFNNRR45 pKa = 11.84EE46 pKa = 3.95VEE48 pKa = 4.07NLIEE52 pKa = 4.12HH53 pKa = 6.58HH54 pKa = 6.88RR55 pKa = 11.84FTAKK59 pKa = 8.6PTKK62 pKa = 9.2KK63 pKa = 9.86QLRR66 pKa = 11.84KK67 pKa = 9.34LHH69 pKa = 6.38KK70 pKa = 10.16LSKK73 pKa = 10.38QYY75 pKa = 10.93LINDD79 pKa = 3.39INKK82 pKa = 9.72

Molecular weight: 9.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

16434

37

1039

188.9

21.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.128 ± 0.58

1.406 ± 0.138

6.249 ± 0.233

7.016 ± 0.246

3.98 ± 0.195

6.48 ± 0.238

1.74 ± 0.188

7.192 ± 0.265

7.138 ± 0.225

7.917 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.829 ± 0.149

6.243 ± 0.263

3.535 ± 0.23

3.602 ± 0.42

4.679 ± 0.189

6.128 ± 0.225

5.75 ± 0.206

6.456 ± 0.249

1.448 ± 0.124

4.077 ± 0.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski