Wheat yellow striate virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Betarhabdovirinae; Alphanucleorhabdovirus; Wheat yellow striate alphanucleorhabdovirus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R4K2H4|A0A2R4K2H4_9RHAB Nucleocapsid protein OS=Wheat yellow striate virus OX=2152660 PE=4 SV=1
MM1 pKa = 8.05DD2 pKa = 5.28KK3 pKa = 11.07SLDD6 pKa = 3.82NKK8 pKa = 10.62QGDD11 pKa = 4.45LLKK14 pKa = 10.07MSKK17 pKa = 8.38PTADD21 pKa = 3.3TGSPDD26 pKa = 3.65TPHH29 pKa = 7.66PEE31 pKa = 3.98PAHH34 pKa = 5.51TPEE37 pKa = 5.57GDD39 pKa = 3.29TTQKK43 pKa = 11.02EE44 pKa = 4.32DD45 pKa = 3.36TKK47 pKa = 11.51GNTGKK52 pKa = 10.64EE53 pKa = 3.87EE54 pKa = 3.96AAQSPEE60 pKa = 4.03AEE62 pKa = 4.48QEE64 pKa = 4.39DD65 pKa = 4.14DD66 pKa = 4.7TQPEE70 pKa = 4.37WCCEE74 pKa = 3.71IEE76 pKa = 4.13EE77 pKa = 5.12LDD79 pKa = 5.01VEE81 pKa = 4.66SDD83 pKa = 3.1WEE85 pKa = 4.03FCYY88 pKa = 10.58RR89 pKa = 11.84EE90 pKa = 4.19EE91 pKa = 6.18DD92 pKa = 3.47FDD94 pKa = 5.99CYY96 pKa = 10.81FDD98 pKa = 4.18NVWVYY103 pKa = 10.73EE104 pKa = 5.27LIDD107 pKa = 3.61EE108 pKa = 4.56CNGWRR113 pKa = 11.84EE114 pKa = 3.82

Molecular weight: 13.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R4K2I6|A0A2R4K2I6_9RHAB GDP polyribonucleotidyltransferase OS=Wheat yellow striate virus OX=2152660 PE=4 SV=1
MM1 pKa = 7.85ADD3 pKa = 3.76SLDD6 pKa = 3.69TDD8 pKa = 3.84RR9 pKa = 11.84GRR11 pKa = 11.84TTRR14 pKa = 11.84SGSKK18 pKa = 10.26LKK20 pKa = 10.75AQTTHH25 pKa = 6.99RR26 pKa = 11.84VGTSSGSVRR35 pKa = 11.84GGKK38 pKa = 9.44PYY40 pKa = 10.83EE41 pKa = 4.15KK42 pKa = 10.31DD43 pKa = 3.56FKK45 pKa = 10.91AVEE48 pKa = 4.02ARR50 pKa = 11.84FHH52 pKa = 6.28NFDD55 pKa = 5.6PISASIIRR63 pKa = 11.84GDD65 pKa = 4.02QEE67 pKa = 3.97GTKK70 pKa = 10.41ADD72 pKa = 5.09LIMSDD77 pKa = 3.44SSVTKK82 pKa = 9.56ATAPAEE88 pKa = 4.24AAAPPPPPQPAAVPLTPPASATTQGQKK115 pKa = 10.36RR116 pKa = 11.84PGDD119 pKa = 3.76PEE121 pKa = 3.94TLEE124 pKa = 4.23EE125 pKa = 4.23GNAAKK130 pKa = 10.2RR131 pKa = 11.84VQRR134 pKa = 11.84ANKK137 pKa = 9.22VNNLLTTQGIGEE149 pKa = 4.22PSKK152 pKa = 11.01SAISQYY158 pKa = 10.69VVARR162 pKa = 11.84LNANNVEE169 pKa = 4.36HH170 pKa = 7.67DD171 pKa = 3.81DD172 pKa = 4.58AMVAEE177 pKa = 4.78CANMAVYY184 pKa = 9.67GWKK187 pKa = 9.88EE188 pKa = 3.61GKK190 pKa = 10.3KK191 pKa = 9.94FVSTQILNQATTVIPDD207 pKa = 5.42LITSMVTNANLITNAANALNSIPDD231 pKa = 3.69KK232 pKa = 10.65VAGAIRR238 pKa = 11.84TNIEE242 pKa = 3.47HH243 pKa = 6.79VAFQTGSKK251 pKa = 8.26ATKK254 pKa = 9.48RR255 pKa = 11.84DD256 pKa = 3.52TLVRR260 pKa = 11.84TAEE263 pKa = 4.28SIYY266 pKa = 10.75QNAAAEE272 pKa = 4.41SKK274 pKa = 11.17VDD276 pKa = 4.46FINNFIISAGINIQEE291 pKa = 4.54VKK293 pKa = 10.74RR294 pKa = 11.84NVANYY299 pKa = 7.88RR300 pKa = 11.84TIANAIIKK308 pKa = 10.26RR309 pKa = 11.84NTVLNIINEE318 pKa = 4.49TTEE321 pKa = 3.93HH322 pKa = 6.04QALLTQVQGNKK333 pKa = 10.16DD334 pKa = 4.29DD335 pKa = 3.43IRR337 pKa = 11.84NIARR341 pKa = 11.84GLSASYY347 pKa = 10.57VII349 pKa = 5.25

Molecular weight: 37.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4238

114

1965

605.4

68.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.521 ± 1.353

1.628 ± 0.296

6.111 ± 0.509

5.757 ± 0.681

3.115 ± 0.246

6.371 ± 0.351

2.076 ± 0.236

7.315 ± 0.64

5.781 ± 0.462

8.66 ± 0.843

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.539 ± 0.345

5.002 ± 0.48

4.625 ± 0.643

3.728 ± 0.673

5.05 ± 0.439

7.551 ± 0.88

6.914 ± 0.497

5.923 ± 0.204

1.534 ± 0.228

3.799 ± 0.353
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski