Influenza A virus (A/gyrfalcon/Washington/41088-6/2014(H5N8))

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; Influenza A virus; H5N8 subtype

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C4X0C0|A0A0C4X0C0_9INFA Hemagglutinin OS=Influenza A virus (A/gyrfalcon/Washington/41088-6/2014(H5N8)) OX=1589663 GN=HA PE=1 SV=1
MM1 pKa = 7.38SLLTEE6 pKa = 4.41VEE8 pKa = 4.5TPTRR12 pKa = 11.84TEE14 pKa = 3.93WEE16 pKa = 4.58CRR18 pKa = 11.84CSDD21 pKa = 3.35SSDD24 pKa = 3.75PLVVAANIIGILHH37 pKa = 7.21LILWILDD44 pKa = 3.39RR45 pKa = 11.84LFFKK49 pKa = 10.78CIYY52 pKa = 9.95RR53 pKa = 11.84RR54 pKa = 11.84LKK56 pKa = 10.19YY57 pKa = 10.03GLKK60 pKa = 9.88IGPSTEE66 pKa = 4.06GVPEE70 pKa = 4.24SMRR73 pKa = 11.84EE74 pKa = 3.92EE75 pKa = 4.1YY76 pKa = 10.41RR77 pKa = 11.84QEE79 pKa = 3.74QQSAVDD85 pKa = 3.3VDD87 pKa = 4.47DD88 pKa = 3.92GHH90 pKa = 6.42FVNIEE95 pKa = 3.83LEE97 pKa = 4.08

Molecular weight: 11.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C4WPG8|A0A0C4WPG8_9INFA Nucleoprotein OS=Influenza A virus (A/gyrfalcon/Washington/41088-6/2014(H5N8)) OX=1589663 GN=NP PE=3 SV=1
MM1 pKa = 7.36EE2 pKa = 6.17RR3 pKa = 11.84IKK5 pKa = 10.51EE6 pKa = 4.0LRR8 pKa = 11.84DD9 pKa = 3.29LMSQSRR15 pKa = 11.84TRR17 pKa = 11.84EE18 pKa = 3.86ILTKK22 pKa = 8.81TTVDD26 pKa = 2.86HH27 pKa = 5.76MAIIKK32 pKa = 10.06KK33 pKa = 7.84YY34 pKa = 8.29TSGRR38 pKa = 11.84QEE40 pKa = 3.99KK41 pKa = 10.64NPALRR46 pKa = 11.84MKK48 pKa = 10.19WMMAMKK54 pKa = 10.45YY55 pKa = 10.07PITADD60 pKa = 3.19KK61 pKa = 10.67RR62 pKa = 11.84IMEE65 pKa = 4.94MIPEE69 pKa = 4.3RR70 pKa = 11.84NEE72 pKa = 3.52QGQTLWSKK80 pKa = 9.91TNDD83 pKa = 3.44AGSDD87 pKa = 3.19RR88 pKa = 11.84VMVSPLAVTWWNRR101 pKa = 11.84NGPTASTVHH110 pKa = 5.52YY111 pKa = 8.66PKK113 pKa = 10.49VYY115 pKa = 8.32KK116 pKa = 10.23TYY118 pKa = 9.43FEE120 pKa = 4.01KK121 pKa = 11.11VEE123 pKa = 4.08RR124 pKa = 11.84LKK126 pKa = 11.04HH127 pKa = 4.72GTFGPVHH134 pKa = 6.73FRR136 pKa = 11.84NQIKK140 pKa = 9.7IRR142 pKa = 11.84RR143 pKa = 11.84RR144 pKa = 11.84VDD146 pKa = 3.14INPGHH151 pKa = 7.33ADD153 pKa = 3.84LSAKK157 pKa = 9.4EE158 pKa = 3.98AQDD161 pKa = 3.52VIMEE165 pKa = 4.23VVFPNEE171 pKa = 3.21VGARR175 pKa = 11.84ILTSEE180 pKa = 4.22SQLTITKK187 pKa = 9.33EE188 pKa = 4.15KK189 pKa = 10.77KK190 pKa = 10.27EE191 pKa = 3.94EE192 pKa = 3.95LQDD195 pKa = 3.83CKK197 pKa = 10.5IAPLMVAYY205 pKa = 9.47MLEE208 pKa = 4.18RR209 pKa = 11.84EE210 pKa = 4.67LVRR213 pKa = 11.84KK214 pKa = 7.77TRR216 pKa = 11.84FLPVAGGTSSVYY228 pKa = 10.37IEE230 pKa = 4.48VLHH233 pKa = 5.98LTQGTCWEE241 pKa = 4.0QMYY244 pKa = 9.45TPGGKK249 pKa = 9.01VRR251 pKa = 11.84NDD253 pKa = 4.03DD254 pKa = 3.41VDD256 pKa = 3.29QSLIIAARR264 pKa = 11.84NIVRR268 pKa = 11.84RR269 pKa = 11.84ATVSADD275 pKa = 3.25PLASLLEE282 pKa = 4.46MCHH285 pKa = 5.37STQIGGTRR293 pKa = 11.84MVDD296 pKa = 3.19ILRR299 pKa = 11.84QNPTEE304 pKa = 3.98EE305 pKa = 4.03QAVDD309 pKa = 2.95ICKK312 pKa = 10.24AAMGLRR318 pKa = 11.84ISSSFSFGGFTFKK331 pKa = 10.07RR332 pKa = 11.84TSGSSVRR339 pKa = 11.84KK340 pKa = 9.53EE341 pKa = 3.93EE342 pKa = 4.01EE343 pKa = 4.05VLTGNLQTLKK353 pKa = 10.42IRR355 pKa = 11.84VHH357 pKa = 5.94EE358 pKa = 4.86GYY360 pKa = 10.8EE361 pKa = 3.92EE362 pKa = 3.93FTMVGRR368 pKa = 11.84RR369 pKa = 11.84ATAILRR375 pKa = 11.84KK376 pKa = 8.32ATRR379 pKa = 11.84RR380 pKa = 11.84LIQLIVSGRR389 pKa = 11.84DD390 pKa = 3.26EE391 pKa = 3.91QSIAEE396 pKa = 4.44AIIVAMVFSQEE407 pKa = 3.62DD408 pKa = 3.9CMIKK412 pKa = 10.33AVRR415 pKa = 11.84GDD417 pKa = 3.68LNFVNRR423 pKa = 11.84ANQRR427 pKa = 11.84LNPMHH432 pKa = 6.37QLLRR436 pKa = 11.84HH437 pKa = 5.04FQKK440 pKa = 10.58DD441 pKa = 3.4AKK443 pKa = 11.02VLFQNWGIEE452 pKa = 4.2PIDD455 pKa = 3.91NVMGMIGILPDD466 pKa = 3.56MTPSTEE472 pKa = 3.52MSLRR476 pKa = 11.84GVRR479 pKa = 11.84VSKK482 pKa = 10.41MGVDD486 pKa = 4.11EE487 pKa = 4.4YY488 pKa = 11.53SSTEE492 pKa = 3.91RR493 pKa = 11.84VVVSIDD499 pKa = 2.74RR500 pKa = 11.84FLRR503 pKa = 11.84VRR505 pKa = 11.84DD506 pKa = 3.5QQGNVLLSPEE516 pKa = 4.44EE517 pKa = 3.96VSEE520 pKa = 4.17TLGTEE525 pKa = 3.9KK526 pKa = 10.39LTITYY531 pKa = 9.18SSSMMWEE538 pKa = 3.7INGPEE543 pKa = 3.98SVLVNTYY550 pKa = 7.57QWIIRR555 pKa = 11.84NWEE558 pKa = 3.63IVKK561 pKa = 9.75IQWSQDD567 pKa = 2.67PTMLYY572 pKa = 10.5NKK574 pKa = 9.92VEE576 pKa = 4.18FEE578 pKa = 4.34PFQSLVPKK586 pKa = 10.24AARR589 pKa = 11.84GQYY592 pKa = 10.02SGFVRR597 pKa = 11.84TLFQQMRR604 pKa = 11.84DD605 pKa = 3.48VLGTFDD611 pKa = 4.48TIQIIKK617 pKa = 10.05LLPFAAAPPEE627 pKa = 3.99HH628 pKa = 7.31SRR630 pKa = 11.84MQFSSLTVNVRR641 pKa = 11.84GSGMRR646 pKa = 11.84ILVRR650 pKa = 11.84GNSPVFNYY658 pKa = 10.56NKK660 pKa = 8.8ATKK663 pKa = 10.03RR664 pKa = 11.84LAVLGKK670 pKa = 10.11DD671 pKa = 3.15AGALTEE677 pKa = 5.35DD678 pKa = 4.57PDD680 pKa = 3.73EE681 pKa = 4.52GTTGVEE687 pKa = 4.2SAVLRR692 pKa = 11.84GFLILGKK699 pKa = 9.19EE700 pKa = 3.99DD701 pKa = 3.41RR702 pKa = 11.84RR703 pKa = 11.84YY704 pKa = 11.01GPALSINEE712 pKa = 4.34LSNLAKK718 pKa = 10.63GEE720 pKa = 4.09KK721 pKa = 10.32ANVLIGQGDD730 pKa = 3.9VVLVMKK736 pKa = 10.39RR737 pKa = 11.84KK738 pKa = 9.29RR739 pKa = 11.84DD740 pKa = 3.62SSILTDD746 pKa = 3.31SQTATKK752 pKa = 9.89RR753 pKa = 11.84IRR755 pKa = 11.84MVINN759 pKa = 3.84

Molecular weight: 85.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

4565

90

759

415.0

47.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.564 ± 0.462

1.709 ± 0.317

4.556 ± 0.235

7.448 ± 0.548

3.79 ± 0.294

6.572 ± 0.434

1.731 ± 0.208

6.462 ± 0.216

5.783 ± 0.447

8.105 ± 0.504

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.899 ± 0.398

5.323 ± 0.504

3.768 ± 0.211

4.118 ± 0.427

6.966 ± 0.628

7.448 ± 0.271

6.396 ± 0.42

6.002 ± 0.673

1.643 ± 0.196

2.716 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski