Sphingopyxis bauzanensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingopyxis

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3927 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A246JT58|A0A246JT58_9SPHN HTH_17 domain-containing protein OS=Sphingopyxis bauzanensis OX=651663 GN=CDQ92_13380 PE=4 SV=1
MM1 pKa = 8.05ADD3 pKa = 3.93PLDD6 pKa = 3.9MTGLWTGQYY15 pKa = 8.63WYY17 pKa = 9.3PEE19 pKa = 3.89PWEE22 pKa = 4.15PPMGFVATISDD33 pKa = 3.85NAGSLSGTTTEE44 pKa = 5.22ASDD47 pKa = 3.69LFIGVDD53 pKa = 3.51EE54 pKa = 4.66RR55 pKa = 11.84ADD57 pKa = 3.35IRR59 pKa = 11.84GTRR62 pKa = 11.84SGTSVSFYY70 pKa = 10.62KK71 pKa = 10.94YY72 pKa = 10.09YY73 pKa = 10.94DD74 pKa = 3.14GTRR77 pKa = 11.84AYY79 pKa = 9.1GHH81 pKa = 5.95SVTYY85 pKa = 10.34EE86 pKa = 4.11GVLSADD92 pKa = 3.79GQQVEE97 pKa = 4.94GQWSLDD103 pKa = 3.68DD104 pKa = 3.92YY105 pKa = 11.28SGSFIMLRR113 pKa = 11.84SLQQFSVEE121 pKa = 3.84QLEE124 pKa = 4.48EE125 pKa = 4.24FNQLDD130 pKa = 3.72VSVSS134 pKa = 3.22

Molecular weight: 14.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A246K1L8|A0A246K1L8_9SPHN Cell shape determination protein CcmA OS=Sphingopyxis bauzanensis OX=651663 GN=CDQ92_04480 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.43GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.81ATVGGRR28 pKa = 11.84KK29 pKa = 8.83ILANRR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.26KK41 pKa = 10.65LSAA44 pKa = 3.91

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3927

0

3927

1240143

40

2569

315.8

34.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.432 ± 0.062

0.791 ± 0.011

6.21 ± 0.03

5.286 ± 0.035

3.605 ± 0.025

8.778 ± 0.043

1.987 ± 0.02

5.235 ± 0.028

3.138 ± 0.027

9.776 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.452 ± 0.019

2.612 ± 0.027

5.191 ± 0.031

3.075 ± 0.018

7.205 ± 0.04

5.305 ± 0.029

5.261 ± 0.028

7.0 ± 0.032

1.465 ± 0.016

2.195 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski